Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex

The prefrontal cortex plays a key role in the control of higher brain functions and is involved in the pathophysiology and treatment of schizophrenia. Here we report that ∼60% of the neurons in rat and mouse prefrontal cortex express 5-HT1A and/or 5-HT2A receptor mRNAs, which are highly co-localized...

Descripción completa

Detalles Bibliográficos
Autores: Amargós-Bosch, Mercè, Bortolozzi, Analía, Puig, M. Victoria, Serrats, Jordi, Adell, Albert, Celada, Pau, Toth, Miklos, Mengod Los Arcos, Guadalupe, Artigas, Francesc
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2004
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/34683
Acceso en línea:http://hdl.handle.net/10261/34683
Access Level:acceso abierto
Palabra clave:5-HT1A receptors
5-HT 2A receptors
Antipsychotics
Medial prefrontal cortex
Pyramidal neuron
id ES_2a0754ae5530deaa83dd8b0eb4af9fbf
oai_identifier_str oai:digital.csic.es:10261/34683
network_acronym_str ES
network_name_str España
repository_id_str
spelling Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal CortexAmargós-Bosch, MercèBortolozzi, AnalíaPuig, M. VictoriaSerrats, JordiAdell, AlbertCelada, PauToth, MiklosMengod Los Arcos, GuadalupeArtigas, Francesc5-HT1A receptors5-HT 2A receptorsAntipsychoticsMedial prefrontal cortexPyramidal neuronThe prefrontal cortex plays a key role in the control of higher brain functions and is involved in the pathophysiology and treatment of schizophrenia. Here we report that ∼60% of the neurons in rat and mouse prefrontal cortex express 5-HT1A and/or 5-HT2A receptor mRNAs, which are highly co-localized (∼80%). The electrical stimulation of the dorsal and median raphe nuclei elicited 5-HT1A-mediated inhibitions and 5-HT2A-mediated excitations in identified pyramidal neurons recorded extracellularly in rat medial prefrontal cortex (mPFC). Opposite responses in the same pyramidal neuron could be evoked by stimulating the raphe nuclei at different coordinates, suggesting a precise connectivity between 5-HT neuronal subgroups and 5-HT1A and 5-HT2A receptors in pyramidal neurons. Microdialysis experiments showed that the increase in local 5-HT release evoked by the activation of 5-HT2A receptors in mPFC by DOI (5-HT2A/2C receptor agonist) was reversed by co-perfusion of 5-HT1A agonists. This inhibitory effect was antagonized by WAY-100635 and the prior inactivation of 5-HT1A receptors in rats and was absent in mice lacking 5-HT1A receptors. These observations help to clarify the interactions between the mPFC and the raphe nuclei, two key areas in psychiatric illnesses and improve our understanding of the action of atypical antipsychotics, acting through these 5-HT receptors.Peer reviewedOxford University Press201120112004info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/34683reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/cercor/bhg128info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/346832026-05-22T06:33:51Z
dc.title.none.fl_str_mv Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
title Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
spellingShingle Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
Amargós-Bosch, Mercè
5-HT1A receptors
5-HT 2A receptors
Antipsychotics
Medial prefrontal cortex
Pyramidal neuron
title_short Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
title_full Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
title_fullStr Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
title_full_unstemmed Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
title_sort Co-expression and in vivo interaction of Serotonin1A and Serotonin2A receptors in Pyramidal Neurons of Prefrontal Cortex
dc.creator.none.fl_str_mv Amargós-Bosch, Mercè
Bortolozzi, Analía
Puig, M. Victoria
Serrats, Jordi
Adell, Albert
Celada, Pau
Toth, Miklos
Mengod Los Arcos, Guadalupe
Artigas, Francesc
author Amargós-Bosch, Mercè
author_facet Amargós-Bosch, Mercè
Bortolozzi, Analía
Puig, M. Victoria
Serrats, Jordi
Adell, Albert
Celada, Pau
Toth, Miklos
Mengod Los Arcos, Guadalupe
Artigas, Francesc
author_role author
author2 Bortolozzi, Analía
Puig, M. Victoria
Serrats, Jordi
Adell, Albert
Celada, Pau
Toth, Miklos
Mengod Los Arcos, Guadalupe
Artigas, Francesc
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 5-HT1A receptors
5-HT 2A receptors
Antipsychotics
Medial prefrontal cortex
Pyramidal neuron
topic 5-HT1A receptors
5-HT 2A receptors
Antipsychotics
Medial prefrontal cortex
Pyramidal neuron
description The prefrontal cortex plays a key role in the control of higher brain functions and is involved in the pathophysiology and treatment of schizophrenia. Here we report that ∼60% of the neurons in rat and mouse prefrontal cortex express 5-HT1A and/or 5-HT2A receptor mRNAs, which are highly co-localized (∼80%). The electrical stimulation of the dorsal and median raphe nuclei elicited 5-HT1A-mediated inhibitions and 5-HT2A-mediated excitations in identified pyramidal neurons recorded extracellularly in rat medial prefrontal cortex (mPFC). Opposite responses in the same pyramidal neuron could be evoked by stimulating the raphe nuclei at different coordinates, suggesting a precise connectivity between 5-HT neuronal subgroups and 5-HT1A and 5-HT2A receptors in pyramidal neurons. Microdialysis experiments showed that the increase in local 5-HT release evoked by the activation of 5-HT2A receptors in mPFC by DOI (5-HT2A/2C receptor agonist) was reversed by co-perfusion of 5-HT1A agonists. This inhibitory effect was antagonized by WAY-100635 and the prior inactivation of 5-HT1A receptors in rats and was absent in mice lacking 5-HT1A receptors. These observations help to clarify the interactions between the mPFC and the raphe nuclei, two key areas in psychiatric illnesses and improve our understanding of the action of atypical antipsychotics, acting through these 5-HT receptors.
publishDate 2004
dc.date.none.fl_str_mv 2004
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/34683
url http://hdl.handle.net/10261/34683
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/cercor/bhg128
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405044611219456
score 15,811543

Similares en LA Referencia