3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration

The hippocampus plays a key role in contextual conditioning and has been proposed as an important component of the cocaine addiction brain circuit. To gain knowledge about cocaine-induced alterations in this circuit, we used focused ion beam milling/scanning electron microscopy to reveal and quantif...

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Detalles Bibliográficos
Autores: Blázquez-Llorca, Lidia, Miguéns, Miguel, Montero-Crespo, M., Selvas, A., González-Soriano, Juncal, Ambrosio, E., DeFelipe, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/257591
Acceso en línea:http://hdl.handle.net/10261/257591
Access Level:acceso abierto
Palabra clave:addiction
drug
FIB/SEM
hippocampus
synapse
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spelling 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-AdministrationBlázquez-Llorca, LidiaMiguéns, MiguelMontero-Crespo, M.Selvas, A.González-Soriano, JuncalAmbrosio, E.DeFelipe, JavieraddictiondrugFIB/SEMhippocampussynapseThe hippocampus plays a key role in contextual conditioning and has been proposed as an important component of the cocaine addiction brain circuit. To gain knowledge about cocaine-induced alterations in this circuit, we used focused ion beam milling/scanning electron microscopy to reveal and quantify the three-dimensional synaptic organization of the neuropil of the stratum radiatum of the rat CA1, under normal circumstances and after cocaine-self administration (SA). Most synapses are asymmetric (excitatory), macular-shaped, and in contact with dendritic spine heads. After cocaine-SA, the size and the complexity of the shape of both asymmetric and symmetric (inhibitory) synapses increased but no changes were observed in the synaptic density. This work constitutes the first detailed report on the 3D synaptic organization in the stratum radiatum of the CA1 field of cocaine-SA rats. Our data contribute to the elucidation of the normal and altered synaptic organization of the hippocampus, which is crucial for better understanding the neurobiological mechanisms underlying cocaine addiction.Ministerio de Ciencia e Innovación (PSI2016-80541-P to E.A.); Ministerio de Sanidad, Servicios Sociales e Igualdad (Red de Trastornos Adictivos [RTA-RD16/0017/0022 del Instituto de Salud Carlos III to E.A.] and Plan Nacional sobre Drogas [2016I073 to E.A.]); UNED (Plan de Promoción de la Investigación, 2014-040-UNED-POST to L.B.-L.); UNED-Banco Santander (“Independent Thinking”, 2017-VICE-0012 to L.B.- L.); European Union (JUST-2017-AG-DRUGS-806996-JUSTSO to E.A.); Centro de Investigación en Red sobre Enfermedades Neurodegenerativas (CIBERNED, CB06/05/0066 to J.D.); Ministerio de Ciencia, Innovación y Universidades (PGC2018-094307-B-I00 to J.D. and the Cajal Blue Brain Project [the Spanish partner of the Blue Brain Project initiative from EPFL, Switzerland] to J.D.); and Ministerio de Educación, Cultura y Deporte (contract FPU14/02245 to M.M.-C.).Oxford University PressMinisterio de Ciencia e Innovación (España)Ministerio de Sanidad, Servicios Sociales e Igualdad (España)Plan Nacional sobre Drogas (España)Universidad Nacional de Educación a Distancia (España)European CommissionCentro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España)Ministerio de Ciencia, Innovación y Universidades (España)Cajal Blue BrainMinisterio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/257591reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/cercor/bhaa331Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2575912026-05-22T06:33:51Z
dc.title.none.fl_str_mv 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
title 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
spellingShingle 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
Blázquez-Llorca, Lidia
addiction
drug
FIB/SEM
hippocampus
synapse
title_short 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
title_full 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
title_fullStr 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
title_full_unstemmed 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
title_sort 3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration
dc.creator.none.fl_str_mv Blázquez-Llorca, Lidia
Miguéns, Miguel
Montero-Crespo, M.
Selvas, A.
González-Soriano, Juncal
Ambrosio, E.
DeFelipe, Javier
author Blázquez-Llorca, Lidia
author_facet Blázquez-Llorca, Lidia
Miguéns, Miguel
Montero-Crespo, M.
Selvas, A.
González-Soriano, Juncal
Ambrosio, E.
DeFelipe, Javier
author_role author
author2 Miguéns, Miguel
Montero-Crespo, M.
Selvas, A.
González-Soriano, Juncal
Ambrosio, E.
DeFelipe, Javier
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Sanidad, Servicios Sociales e Igualdad (España)
Plan Nacional sobre Drogas (España)
Universidad Nacional de Educación a Distancia (España)
European Commission
Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Cajal Blue Brain
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv addiction
drug
FIB/SEM
hippocampus
synapse
topic addiction
drug
FIB/SEM
hippocampus
synapse
description The hippocampus plays a key role in contextual conditioning and has been proposed as an important component of the cocaine addiction brain circuit. To gain knowledge about cocaine-induced alterations in this circuit, we used focused ion beam milling/scanning electron microscopy to reveal and quantify the three-dimensional synaptic organization of the neuropil of the stratum radiatum of the rat CA1, under normal circumstances and after cocaine-self administration (SA). Most synapses are asymmetric (excitatory), macular-shaped, and in contact with dendritic spine heads. After cocaine-SA, the size and the complexity of the shape of both asymmetric and symmetric (inhibitory) synapses increased but no changes were observed in the synaptic density. This work constitutes the first detailed report on the 3D synaptic organization in the stratum radiatum of the CA1 field of cocaine-SA rats. Our data contribute to the elucidation of the normal and altered synaptic organization of the hippocampus, which is crucial for better understanding the neurobiological mechanisms underlying cocaine addiction.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/257591
url http://hdl.handle.net/10261/257591
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/cercor/bhaa331
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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