Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity

21 págs.- 4 fig..- 2 tablas.- 77 referencias

Detalles Bibliográficos
Autores: Lara Moreno, Alba, Aguilar Romero, Inés María, Madrid Díaz, Fernando, Villaverde Capellán, J., Carlier, Jorge D., Santos, Juan Luis, Alonso, Esteban, Morillo González, Esmeralda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394136
Acceso en línea:http://hdl.handle.net/10261/394136
Access Level:acceso abierto
Palabra clave:Nonylphenol
Biodegradation
Mineralization
Sewage sludge
Bacillus safensis
Cyclodextrins
id ES_29f7b09c131bde520a88ddcac8ee68cb
oai_identifier_str oai:digital.csic.es:10261/394136
network_acronym_str ES
network_name_str España
repository_id_str
spelling Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and ToxicityLara Moreno, AlbaAguilar Romero, Inés MaríaMadrid Díaz, FernandoVillaverde Capellán, J.Carlier, Jorge D.Santos, Juan LuisAlonso, EstebanMorillo González, EsmeraldaNonylphenolBiodegradationMineralizationSewage sludgeBacillus safensisCyclodextrins21 págs.- 4 fig..- 2 tablas.- 77 referencias4-Nonylphenols (4-NPs) are persistent endocrine disruptors frequently found in wastewater treatment plant (WWTP) effluents and sewage sludge. This study evaluated the ability of eight bacterial strains that were isolated from sewage sludge to degrade 4-n-NP in an aqueous solution. Bacillus safensis CN12, Shewanella putrefaciens CN17, and Alcaligenes faecalis CN8 showed the highest degradation rates, removing 100%, 75%, and 74% of 4-n-NP (10 mg L−1 ), with DT50 values of 0.90, 8.9, and 10.4 days, respectively. Despite the reduction in 4-n-NP concentrations, ecotoxicity assays revealed that the resulting transformation products (TPs) were more toxic than the parent compound. To investigate the potential degradation mechanisms, in silico and gene expression analyses were conducted on B. safensis CN12, revealing a significant upregulation of the multicopper oxidase gene, cotA (7.25-fold), and the ring-cleaving dioxygenase gene, mhqO (13.9-fold). Although the CN12 strain showed potential for mineralization based on gene expression studies, this was not observed in the aqueous solution. However, when 4-n-NP was adsorbed on sludge and treated with CN12 in the presence of hydroxypropyl-β-cyclodextrin (HPBCD) as a bioavailability enhancer, mineralization reached up to 33%, indicating a synergistic effect with the native sludge microbiota.This research was funded by Junta de Andalucía (Council of Economic Transformation, Industry, Knowledge and University) under the research project PY20_01069 (FEDER Andalucía PO 2014-2020) and by Portuguese National Funds from FCT- Fundação para a Ciência e a Tecnologia, I.P., within the scope of the project PTDC/CTA-AMB/7782/2020.Peer reviewedMultidisciplinary Digital Publishing InstituteJunta de AndalucíaFundação para a Ciência e a Tecnologia (Portugal)Lara Moreno, Alba [0000-0002-5741-7662]Aguilar Romero, Inés María [0000-0001-7786-8172]Madrid Díaz, Fernando [0000-0002-2921-3515]Villaverde Capellán, J. [0000-0002-8694-7929]Morillo González, Esmeralda [0000-0002-4485-2315]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/394136reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/Junta de Andalucía/Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020)/PY20_01069http://dx.doi.org/10.3390/app15126408Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3941362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
title Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
spellingShingle Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
Lara Moreno, Alba
Nonylphenol
Biodegradation
Mineralization
Sewage sludge
Bacillus safensis
Cyclodextrins
title_short Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
title_full Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
title_fullStr Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
title_full_unstemmed Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
title_sort Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
dc.creator.none.fl_str_mv Lara Moreno, Alba
Aguilar Romero, Inés María
Madrid Díaz, Fernando
Villaverde Capellán, J.
Carlier, Jorge D.
Santos, Juan Luis
Alonso, Esteban
Morillo González, Esmeralda
author Lara Moreno, Alba
author_facet Lara Moreno, Alba
Aguilar Romero, Inés María
Madrid Díaz, Fernando
Villaverde Capellán, J.
Carlier, Jorge D.
Santos, Juan Luis
Alonso, Esteban
Morillo González, Esmeralda
author_role author
author2 Aguilar Romero, Inés María
Madrid Díaz, Fernando
Villaverde Capellán, J.
Carlier, Jorge D.
Santos, Juan Luis
Alonso, Esteban
Morillo González, Esmeralda
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Fundação para a Ciência e a Tecnologia (Portugal)
Lara Moreno, Alba [0000-0002-5741-7662]
Aguilar Romero, Inés María [0000-0001-7786-8172]
Madrid Díaz, Fernando [0000-0002-2921-3515]
Villaverde Capellán, J. [0000-0002-8694-7929]
Morillo González, Esmeralda [0000-0002-4485-2315]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Nonylphenol
Biodegradation
Mineralization
Sewage sludge
Bacillus safensis
Cyclodextrins
topic Nonylphenol
Biodegradation
Mineralization
Sewage sludge
Bacillus safensis
Cyclodextrins
description 21 págs.- 4 fig..- 2 tablas.- 77 referencias
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394136
url http://hdl.handle.net/10261/394136
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/Junta de Andalucía/Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020)/PY20_01069
http://dx.doi.org/10.3390/app15126408
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405040860463104
score 15.198674

Similares en LA Referencia