Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery

Microalgae can produce biostimulants in form of phytohormones, which are compounds that, even if applied in low concentrations, can have stimulant effects on plants growth and can enhance their quality and their resistance to stress. Considering that microalgal biomass can grow recovering nutrients...

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Autores: Álvarez González, Ana, Morais, Etiele Greque de|||0000-0003-2993-8572, Planas Carbonell, Anna, Uggetti, Enrica|||0000-0001-6919-485X
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/398961
Acceso en línea:https://hdl.handle.net/2117/398961
https://dx.doi.org/10.1016/j.scitotenv.2023.166878
Access Level:acceso abierto
Palabra clave:Microalgae -- Biotechnology
Biostimulants
Circular economy
Municipal wastewater
Nutrients recovery
Phytohormones
Sustainable production
Microalgues -- Biotecnologia
Àrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental::Tractament de l'aigua
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spelling Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recoveryÁlvarez González, AnaMorais, Etiele Greque de|||0000-0003-2993-8572Planas Carbonell, AnnaUggetti, Enrica|||0000-0001-6919-485XMicroalgae -- BiotechnologyBiostimulantsCircular economyMunicipal wastewaterNutrients recoveryPhytohormonesSustainable productionMicroalgues -- BiotecnologiaÀrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental::Tractament de l'aiguaMicroalgae can produce biostimulants in form of phytohormones, which are compounds that, even if applied in low concentrations, can have stimulant effects on plants growth and can enhance their quality and their resistance to stress. Considering that microalgal biomass can grow recovering nutrients from wastewater, this circular approach allows to use residues for the production of high added value compounds (such as phytohormones) at low cost. The interest on biostimulants production from microalgae have recently raised. Scientists are focused on the direct application of these cellular extracts on plants, while the number of studies on the identification of bioactive molecules, such as phytohormones, is very scarce. Two cyanobacteria strains (Synechocystis sp. (SY) and Phormidium sp. (PH)) and a chlorophyte (Scenedesmus sp. (SC)) were cultured in laboratory-scale PBRs with a working volume of 2.5 L in secondary urban wastewater varying N:P ratio in the cultures to obtain the highest productivity. The variation of N:P ratio affects microalgae growth, and SY and PH presented higher productivities (73 and 48 mg L-1 d, respectively) under higher N:P ratio (> 22:1). Microalgal biomass was freeze-dried and phytohormones content was measured with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The three microalgae showed similar phytohormones profiles, being the auxin (indole-3-acetic acid, IAA) the most abundant (72 ng g-1DW in SY). Proteins were major macronutrient for all strains, reaching 48 %DW in PH culture. To optimize the biostimulants production, a balance between the production of such compounds, biomass productivity and nutrients removal should be taken into consideration. In this sense, SC was the most promising strain, showing the highest N and P removal rates (73 % and 59 %, respectively) while producing phytohormones.This research was supported by the Spanish Ministry of Science and Innovation (CYRCLE-PID2020-113866RA-I00). E. Uggetti would like to thank the Spanish Ministry of Industry and Economy for her research grant (RYC2018-025514-I). A. Álvarez-González kindly acknowledge the Departament de Recerca i Universitats de la Generalitat de Catalunya for her PhD scholarship (FI AGAUR 2022FI_B 00488).Peer ReviewedElsevier20232023-12-0120242024-01-09journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/398961https://dx.doi.org/10.1016/j.scitotenv.2023.166878reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengAgencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-113866RA-I00 PRODUCCION DE BIOESTIMULANTES A PARTIR DE CIANOBACTERIAS Y AGUA RESIDUAL EN EL MARCO DE LA BIOECONOMIA CIRCULARopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3989612026-05-27T15:37:01Z
dc.title.none.fl_str_mv Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
title Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
spellingShingle Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
Álvarez González, Ana
Microalgae -- Biotechnology
Biostimulants
Circular economy
Municipal wastewater
Nutrients recovery
Phytohormones
Sustainable production
Microalgues -- Biotecnologia
Àrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental::Tractament de l'aigua
title_short Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
title_full Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
title_fullStr Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
title_full_unstemmed Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
title_sort Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery
dc.creator.none.fl_str_mv Álvarez González, Ana
Morais, Etiele Greque de|||0000-0003-2993-8572
Planas Carbonell, Anna
Uggetti, Enrica|||0000-0001-6919-485X
author Álvarez González, Ana
author_facet Álvarez González, Ana
Morais, Etiele Greque de|||0000-0003-2993-8572
Planas Carbonell, Anna
Uggetti, Enrica|||0000-0001-6919-485X
author_role author
author2 Morais, Etiele Greque de|||0000-0003-2993-8572
Planas Carbonell, Anna
Uggetti, Enrica|||0000-0001-6919-485X
author2_role author
author
author
dc.subject.none.fl_str_mv Microalgae -- Biotechnology
Biostimulants
Circular economy
Municipal wastewater
Nutrients recovery
Phytohormones
Sustainable production
Microalgues -- Biotecnologia
Àrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental::Tractament de l'aigua
topic Microalgae -- Biotechnology
Biostimulants
Circular economy
Municipal wastewater
Nutrients recovery
Phytohormones
Sustainable production
Microalgues -- Biotecnologia
Àrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental::Tractament de l'aigua
description Microalgae can produce biostimulants in form of phytohormones, which are compounds that, even if applied in low concentrations, can have stimulant effects on plants growth and can enhance their quality and their resistance to stress. Considering that microalgal biomass can grow recovering nutrients from wastewater, this circular approach allows to use residues for the production of high added value compounds (such as phytohormones) at low cost. The interest on biostimulants production from microalgae have recently raised. Scientists are focused on the direct application of these cellular extracts on plants, while the number of studies on the identification of bioactive molecules, such as phytohormones, is very scarce. Two cyanobacteria strains (Synechocystis sp. (SY) and Phormidium sp. (PH)) and a chlorophyte (Scenedesmus sp. (SC)) were cultured in laboratory-scale PBRs with a working volume of 2.5 L in secondary urban wastewater varying N:P ratio in the cultures to obtain the highest productivity. The variation of N:P ratio affects microalgae growth, and SY and PH presented higher productivities (73 and 48 mg L-1 d, respectively) under higher N:P ratio (> 22:1). Microalgal biomass was freeze-dried and phytohormones content was measured with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The three microalgae showed similar phytohormones profiles, being the auxin (indole-3-acetic acid, IAA) the most abundant (72 ng g-1DW in SY). Proteins were major macronutrient for all strains, reaching 48 %DW in PH culture. To optimize the biostimulants production, a balance between the production of such compounds, biomass productivity and nutrients removal should be taken into consideration. In this sense, SC was the most promising strain, showing the highest N and P removal rates (73 % and 59 %, respectively) while producing phytohormones.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-12-01
2024
2024-01-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/398961
https://dx.doi.org/10.1016/j.scitotenv.2023.166878
url https://hdl.handle.net/2117/398961
https://dx.doi.org/10.1016/j.scitotenv.2023.166878
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-113866RA-I00 PRODUCCION DE BIOESTIMULANTES A PARTIR DE CIANOBACTERIAS Y AGUA RESIDUAL EN EL MARCO DE LA BIOECONOMIA CIRCULAR
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
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