Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer

BackgroundHematological cytometers with a biological fluid module could potentially correct the limitations of the manual chamber method. This study evaluates the agreement between the manual technique and the Sysmex XN-1000 analyzer for white blood cell (WBC) and red blood cell (RBC) counts, as wel...

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Autores: Aguadero, V, Cano-Corres, R, Berlanga, E, Torra, M
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Institut d'Investigació i Innovació Parc Taulí (I3PT)
Repositorio:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
OAI Identifier:oai:i3pt.fundanetsuite.com:p5559
Acceso en línea:https://i3pt.portalinvestigacion.com/publicaciones/5559
Access Level:acceso abierto
Palabra clave:cell counting
hematology
absolute cell count
Flow Cytometry
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spelling Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzerAguadero, VCano-Corres, RBerlanga, ETorra, Mcell countinghematologyabsolute cell countFlow CytometryBackgroundHematological cytometers with a biological fluid module could potentially correct the limitations of the manual chamber method. This study evaluates the agreement between the manual technique and the Sysmex XN-1000 analyzer for white blood cell (WBC) and red blood cell (RBC) counts, as well as for leukocyte differentiation in different types of fluids. This study also evaluates the advantages of incorporating the technique in routine laboratory work. MethodsOne hundred and three fluid samples examined were 45 ascite (AF), 21 synovial (SF), 33 pleural (PF), and 31 cerebrospinal (CSF) fluid samples. All cell counting was performed with a Sysmex XN-1000 and a Fuchs-Rosenthal counting chamber. May Grundwald-Giemsa stain was used for manual WBC differentiation. The manual analysis data were obtained in duplicate by the same two observers. Passing-Bablok regression and the Kappa index were used to evaluate the interchangeability and concordance. ResultsGood agreement was observed for WBC differentiation in all fluids and for WBC counts in SF and PF. An optimal Kappa index was obtained, which indicated agreement and clinical significance for WBC and RBC counts in CSF and for RBC counts in PF. There was disagreement for WBC and RBC analysis in AF, with significantly higher results from the Sysmex XN-1000 than from the manual method. A reduction in laboratory response time was observed when using the automatic method. ConclusionsExcept for AF, the Sysmex XN-1000 results agree with those of the manual method, although to different degrees depending on the fluid type. (c) 2017 International Clinical Cytometry SocietyWILEY2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://i3pt.portalinvestigacion.com/publicaciones/5559CYTOMETRY PART B-CLINICAL CYTOMETRYISSN: 15524949ISSNe: 15524957reponame:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulíinstname:Institut d'Investigació i Innovació Parc Taulí (I3PT)Inglésinfo:eu-repo/semantics/openAccessoai:i3pt.fundanetsuite.com:p55592026-06-21T15:30:37Z
dc.title.none.fl_str_mv Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
title Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
spellingShingle Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
Aguadero, V
cell counting
hematology
absolute cell count
Flow Cytometry
title_short Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
title_full Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
title_fullStr Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
title_full_unstemmed Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
title_sort Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
dc.creator.none.fl_str_mv Aguadero, V
Cano-Corres, R
Berlanga, E
Torra, M
author Aguadero, V
author_facet Aguadero, V
Cano-Corres, R
Berlanga, E
Torra, M
author_role author
author2 Cano-Corres, R
Berlanga, E
Torra, M
author2_role author
author
author
dc.subject.none.fl_str_mv cell counting
hematology
absolute cell count
Flow Cytometry
topic cell counting
hematology
absolute cell count
Flow Cytometry
description BackgroundHematological cytometers with a biological fluid module could potentially correct the limitations of the manual chamber method. This study evaluates the agreement between the manual technique and the Sysmex XN-1000 analyzer for white blood cell (WBC) and red blood cell (RBC) counts, as well as for leukocyte differentiation in different types of fluids. This study also evaluates the advantages of incorporating the technique in routine laboratory work. MethodsOne hundred and three fluid samples examined were 45 ascite (AF), 21 synovial (SF), 33 pleural (PF), and 31 cerebrospinal (CSF) fluid samples. All cell counting was performed with a Sysmex XN-1000 and a Fuchs-Rosenthal counting chamber. May Grundwald-Giemsa stain was used for manual WBC differentiation. The manual analysis data were obtained in duplicate by the same two observers. Passing-Bablok regression and the Kappa index were used to evaluate the interchangeability and concordance. ResultsGood agreement was observed for WBC differentiation in all fluids and for WBC counts in SF and PF. An optimal Kappa index was obtained, which indicated agreement and clinical significance for WBC and RBC counts in CSF and for RBC counts in PF. There was disagreement for WBC and RBC analysis in AF, with significantly higher results from the Sysmex XN-1000 than from the manual method. A reduction in laboratory response time was observed when using the automatic method. ConclusionsExcept for AF, the Sysmex XN-1000 results agree with those of the manual method, although to different degrees depending on the fluid type. (c) 2017 International Clinical Cytometry Society
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://i3pt.portalinvestigacion.com/publicaciones/5559
url https://i3pt.portalinvestigacion.com/publicaciones/5559
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv CYTOMETRY PART B-CLINICAL CYTOMETRY
ISSN: 15524949
ISSNe: 15524957
reponame:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
instname:Institut d'Investigació i Innovació Parc Taulí (I3PT)
instname_str Institut d'Investigació i Innovació Parc Taulí (I3PT)
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