Dysfunctional mitochondrial fission impairs cell reprogramming

We have recently shown that mitochondrial fission is induced early in reprogramming in a Drp1-dependent manner; however, the identity of the factors controlling Drp1 recruitment to mitochondria was unexplored. To investigate this, we used a panel of RNAi targeting factors involved in the regulation...

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Autores: Prieto, J, Leon, M, Ponsoda, X, Garcia-Garcia, F, Bort, R, Serna, E, Barneo-Munoz, M, Palau, F, Dopazo, J, Lopez-Garcia, C, Torres, J
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p13021
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/13021
Access Level:acceso abierto
Palabra clave:cell reprogramming
Gdap1
iPS cells
mitochondrial fission
pluripotency
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oai_identifier_str oai:fisabio.fundanetsuite.com:p13021
network_acronym_str ES
network_name_str España
repository_id_str
spelling Dysfunctional mitochondrial fission impairs cell reprogrammingPrieto, JLeon, MPonsoda, XGarcia-Garcia, FBort, RSerna, EBarneo-Munoz, MPalau, FDopazo, JLopez-Garcia, CTorres, Jcell reprogrammingGdap1iPS cellsmitochondrial fissionpluripotencyWe have recently shown that mitochondrial fission is induced early in reprogramming in a Drp1-dependent manner; however, the identity of the factors controlling Drp1 recruitment to mitochondria was unexplored. To investigate this, we used a panel of RNAi targeting factors involved in the regulation of mitochondrial dynamics and we observed that MiD51, Gdap1 and, to a lesser extent, Mff were found to play key roles in this process. Cells derived from Gdap1-null mice were used to further explore the role of this factor in cell reprogramming. Microarray data revealed a prominent down-regulation of cell cycle pathways in Gdap1-null cells early in reprogramming and cell cycle profiling uncovered a G2/M growth arrest in Gdap1-null cells undergoing reprogramming. High-Content analysis showed that this growth arrest was DNA damage-independent. We propose that lack of efficient mitochondrial fission impairs cell reprogramming by interfering with cell cycle progression in a DNA damage-independent manner.TAYLOR & FRANCIS INC2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/13021CELL CYCLEISSN: 15384101ISSNe: 15514005reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p130212026-06-11T12:45:17Z
dc.title.none.fl_str_mv Dysfunctional mitochondrial fission impairs cell reprogramming
title Dysfunctional mitochondrial fission impairs cell reprogramming
spellingShingle Dysfunctional mitochondrial fission impairs cell reprogramming
Prieto, J
cell reprogramming
Gdap1
iPS cells
mitochondrial fission
pluripotency
title_short Dysfunctional mitochondrial fission impairs cell reprogramming
title_full Dysfunctional mitochondrial fission impairs cell reprogramming
title_fullStr Dysfunctional mitochondrial fission impairs cell reprogramming
title_full_unstemmed Dysfunctional mitochondrial fission impairs cell reprogramming
title_sort Dysfunctional mitochondrial fission impairs cell reprogramming
dc.creator.none.fl_str_mv Prieto, J
Leon, M
Ponsoda, X
Garcia-Garcia, F
Bort, R
Serna, E
Barneo-Munoz, M
Palau, F
Dopazo, J
Lopez-Garcia, C
Torres, J
author Prieto, J
author_facet Prieto, J
Leon, M
Ponsoda, X
Garcia-Garcia, F
Bort, R
Serna, E
Barneo-Munoz, M
Palau, F
Dopazo, J
Lopez-Garcia, C
Torres, J
author_role author
author2 Leon, M
Ponsoda, X
Garcia-Garcia, F
Bort, R
Serna, E
Barneo-Munoz, M
Palau, F
Dopazo, J
Lopez-Garcia, C
Torres, J
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cell reprogramming
Gdap1
iPS cells
mitochondrial fission
pluripotency
topic cell reprogramming
Gdap1
iPS cells
mitochondrial fission
pluripotency
description We have recently shown that mitochondrial fission is induced early in reprogramming in a Drp1-dependent manner; however, the identity of the factors controlling Drp1 recruitment to mitochondria was unexplored. To investigate this, we used a panel of RNAi targeting factors involved in the regulation of mitochondrial dynamics and we observed that MiD51, Gdap1 and, to a lesser extent, Mff were found to play key roles in this process. Cells derived from Gdap1-null mice were used to further explore the role of this factor in cell reprogramming. Microarray data revealed a prominent down-regulation of cell cycle pathways in Gdap1-null cells early in reprogramming and cell cycle profiling uncovered a G2/M growth arrest in Gdap1-null cells undergoing reprogramming. High-Content analysis showed that this growth arrest was DNA damage-independent. We propose that lack of efficient mitochondrial fission impairs cell reprogramming by interfering with cell cycle progression in a DNA damage-independent manner.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/13021
url https://fisabio.portalinvestigacion.com/publicaciones/13021
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv TAYLOR & FRANCIS INC
publisher.none.fl_str_mv TAYLOR & FRANCIS INC
dc.source.none.fl_str_mv CELL CYCLE
ISSN: 15384101
ISSNe: 15514005
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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