Evaluation of enzymatic pretreatment strategies to enhance anaerobic digestion of tomato waste

Tomato residues are a form of solid waste that can be converted into methane through anaerobic digestion (AD). However, methane production is often limited due to incomplete hydrolysis caused by the high lignocellulosic content of tomato waste. Enzymatic pretreatments represent a promising approach...

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Detalles Bibliográficos
Autores: Martínez, María Eugenia, Restovic, Franko, Urrego, Freddy, Fuentes, Derie, Ramos Quiroz, Carlos Antonio|||0000-0002-6683-7175
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/428221
Acceso en línea:https://hdl.handle.net/2117/428221
https://dx.doi.org/10.1002/bbb.2767
Access Level:acceso abierto
Palabra clave:Agroindustry
Circular economy
Enzymes
Methane
Biorefinery
Tomato waste
Anaerobic digestion
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura
Descripción
Sumario:Tomato residues are a form of solid waste that can be converted into methane through anaerobic digestion (AD). However, methane production is often limited due to incomplete hydrolysis caused by the high lignocellulosic content of tomato waste. Enzymatic pretreatments represent a promising approach to enhance methane yields by facilitating substrate hydrolysis. This study evaluated four commercial enzymatic blends – Celluclast 1.5¿L, Maxoliva HC L, Viscozyme, and Novozym 435 – using biomethane potential (BMP) tests with two operational strategies: (i) preincubation of enzymes with tomato waste prior to AD, and (ii) direct addition of enzymes to the anaerobic digester. Maxoliva achieved the highest methane yield (348¿±¿20¿mL CH4 g-1 volatile solids (VS)) under preincubation, representing 99.5% of the theoretical BMP and a 90% increase in comparison with the control. Kinetic analysis using the modified Gompertz equation revealed that Maxoliva also exhibited the highest maximum methane production rate (RMAX¿=¿5.5¿±¿0.2¿mL CH4 g-1 VS day-1) with direct addition. Conversely, Viscozyme showed limited effectiveness, reaching only 47% of the theoretical BMP value. The enhanced methane production observed with certain enzymatic blends is likely attributable to cellulase activity, which facilitates the breakdown of complex carbohydrates into easily biodegradable polysaccharides.