Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study

BACKGROUND NlmCategory: BACKGROUND content: Diarrheagenic Escherichia coli (DEC) are among the leading pathogens associated with endemic diarrhea in low income countries. Yet, few epidemiological studies have focused the contribution of enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC)...

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Autores: Garrine, Marcelino, Matambisso, Glória, Nobela, Nélio, Vubil, Delfino, Massora, Sérgio, Acácio, Sozinho, Nhampossa, Tacilta, Alonso, Pedro, Mandomando, Inácio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183390
Acceso en línea:https://hdl.handle.net/2445/183390
Access Level:acceso abierto
Palabra clave:Infants
Diarrea
Escheríchia coli
Children
Diarrhea
Escherichia coli
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spelling Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control studyGarrine, MarcelinoMatambisso, GlóriaNobela, NélioVubil, DelfinoMassora, SérgioAcácio, SozinhoNhampossa, TaciltaAlonso, PedroMandomando, InácioInfantsDiarreaEscheríchia coliChildrenDiarrheaEscherichia coliBACKGROUND NlmCategory: BACKGROUND content: Diarrheagenic Escherichia coli (DEC) are among the leading pathogens associated with endemic diarrhea in low income countries. Yet, few epidemiological studies have focused the contribution of enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC). - Label: METHODS NlmCategory: METHODS content: "We assessed the contribution of EHEC, EIEC and DAEC isolated from stool samples from a case-control study conducted in children aged <\xE2\x80\x895\xE2\x80\x89years in Southern Mozambique between December 2007 and November 2012. The isolates were screened by conventional PCR targeting stx1 and stx2 (EHEC), ial and ipaH (EIEC), and daaE (DAEC) genes." - Label: RESULTS NlmCategory: RESULTS content: "We analyzed 297 samples from cases with less-severe diarrhea (LSD) matched to 297 controls, and 89 samples from cases with moderate-to-severe diarrhea (MSD) matched to 222 controls, collected between November 3, 2011 and November 2, 2012. DEC were more common among LSD cases (2.7%, [8/297] of cases vs. 1.3% [4/297] of controls; p\xC2\xA0=\xE2\x80\x890.243]) than in MSD cases (0%, [0/89] of cases vs. 0.4%, [1/222]\xC2\xA0of controls; p\xC2\xA0=\xE2\x80\x891.000). Detailed analysis revealed low frequency of EHEC, DAEC or EIEC and no association with diarrhea in all age strata. Although the low frequency, EIEC was predominant in LSD cases aged 24-59\xE2\x80\x89months (4.1% for cases vs. 0% for controls), followed by DAEC in similar frequency for cases and controls in infants (1.9%) and lastly EHEC from one control. Analysis of a subset of samples from previous period (December 10, 2007 and October 31, 2011) showed high frequency of DEC in controls compared to MSD cases (16.2%, [25/154] vs. 11.9%, [14/118], p\xC2\xA0=\xE2\x80\x890.383, respectively). Among these, DAEC predominated, being detected in 7.7% of cases vs. 17.6% of controls aged 24-59\xE2\x80\x89months, followed by EIEC in 7.7% of cases vs. 5.9% of controls for the same age category, although no association was observed. EHEC was detected in one sample from cases and two from controls." - Label: CONCLUSIONS NlmCategory: CONCLUSIONS content: Our data suggests that although EHEC, DAEC and EIEC are less frequent in endemic diarrhea in rural Mozambique, attention should be given to their transmission dynamics (e.g. the role on sporadic or epidemic diarrhea) considering that the role of asymptomatic individuals as source of dissemination remains unknown.BioMed Central2022202220202022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion6application/pdfhttps://hdl.handle.net/2445/183390Articles publicats en revistes (ISGlobal)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1186/s12879-020-05380-1BMC Infectious Diseases, 2020, vol. 20, num. 659http://dx.doi.org/10.1186/s12879-020-05380-1cc by (c) Garrine, Marcelino et al., 2020http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1833902026-05-29T05:05:01Z
dc.title.none.fl_str_mv Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
title Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
spellingShingle Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
Garrine, Marcelino
Infants
Diarrea
Escheríchia coli
Children
Diarrhea
Escherichia coli
title_short Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
title_full Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
title_fullStr Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
title_full_unstemmed Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
title_sort Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique: a case-control study
dc.creator.none.fl_str_mv Garrine, Marcelino
Matambisso, Glória
Nobela, Nélio
Vubil, Delfino
Massora, Sérgio
Acácio, Sozinho
Nhampossa, Tacilta
Alonso, Pedro
Mandomando, Inácio
author Garrine, Marcelino
author_facet Garrine, Marcelino
Matambisso, Glória
Nobela, Nélio
Vubil, Delfino
Massora, Sérgio
Acácio, Sozinho
Nhampossa, Tacilta
Alonso, Pedro
Mandomando, Inácio
author_role author
author2 Matambisso, Glória
Nobela, Nélio
Vubil, Delfino
Massora, Sérgio
Acácio, Sozinho
Nhampossa, Tacilta
Alonso, Pedro
Mandomando, Inácio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Infants
Diarrea
Escheríchia coli
Children
Diarrhea
Escherichia coli
topic Infants
Diarrea
Escheríchia coli
Children
Diarrhea
Escherichia coli
description BACKGROUND NlmCategory: BACKGROUND content: Diarrheagenic Escherichia coli (DEC) are among the leading pathogens associated with endemic diarrhea in low income countries. Yet, few epidemiological studies have focused the contribution of enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC). - Label: METHODS NlmCategory: METHODS content: "We assessed the contribution of EHEC, EIEC and DAEC isolated from stool samples from a case-control study conducted in children aged <\xE2\x80\x895\xE2\x80\x89years in Southern Mozambique between December 2007 and November 2012. The isolates were screened by conventional PCR targeting stx1 and stx2 (EHEC), ial and ipaH (EIEC), and daaE (DAEC) genes." - Label: RESULTS NlmCategory: RESULTS content: "We analyzed 297 samples from cases with less-severe diarrhea (LSD) matched to 297 controls, and 89 samples from cases with moderate-to-severe diarrhea (MSD) matched to 222 controls, collected between November 3, 2011 and November 2, 2012. DEC were more common among LSD cases (2.7%, [8/297] of cases vs. 1.3% [4/297] of controls; p\xC2\xA0=\xE2\x80\x890.243]) than in MSD cases (0%, [0/89] of cases vs. 0.4%, [1/222]\xC2\xA0of controls; p\xC2\xA0=\xE2\x80\x891.000). Detailed analysis revealed low frequency of EHEC, DAEC or EIEC and no association with diarrhea in all age strata. Although the low frequency, EIEC was predominant in LSD cases aged 24-59\xE2\x80\x89months (4.1% for cases vs. 0% for controls), followed by DAEC in similar frequency for cases and controls in infants (1.9%) and lastly EHEC from one control. Analysis of a subset of samples from previous period (December 10, 2007 and October 31, 2011) showed high frequency of DEC in controls compared to MSD cases (16.2%, [25/154] vs. 11.9%, [14/118], p\xC2\xA0=\xE2\x80\x890.383, respectively). Among these, DAEC predominated, being detected in 7.7% of cases vs. 17.6% of controls aged 24-59\xE2\x80\x89months, followed by EIEC in 7.7% of cases vs. 5.9% of controls for the same age category, although no association was observed. EHEC was detected in one sample from cases and two from controls." - Label: CONCLUSIONS NlmCategory: CONCLUSIONS content: Our data suggests that although EHEC, DAEC and EIEC are less frequent in endemic diarrhea in rural Mozambique, attention should be given to their transmission dynamics (e.g. the role on sporadic or epidemic diarrhea) considering that the role of asymptomatic individuals as source of dissemination remains unknown.
publishDate 2020
dc.date.none.fl_str_mv 2020
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183390
url https://hdl.handle.net/2445/183390
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/s12879-020-05380-1
BMC Infectious Diseases, 2020, vol. 20, num. 659
http://dx.doi.org/10.1186/s12879-020-05380-1
dc.rights.none.fl_str_mv cc by (c) Garrine, Marcelino et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Garrine, Marcelino et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (ISGlobal)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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