Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration

The frontiers of bioimaging encompass cutting-edge technologies and methodologies that enhance the visualization, analysis, and interpretation of biological structures and processes with unprecedented precision. In this context, fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerf...

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Authors: Blas Gómez, Sofía, Alonso Moreno, Carlos, Garzón Ruiz, Andrés, Bravo Pérez, Iván
Format: article
Publication Date:2025
Country:España
Institution:Universidad de Castilla-La Mancha
Repository:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/46145
Online Access:https://doi.org/10.1016/j.xinn.2025.101081
https://hdl.handle.net/10578/46145
Access Level:Open access
Keyword:Bioimaging
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spelling Unlocking new frontiers in bioimaging: The power of FLIM-AIE integrationBlas Gómez, SofíaAlonso Moreno, CarlosGarzón Ruiz, AndrésBravo Pérez, IvánBioimagingThe frontiers of bioimaging encompass cutting-edge technologies and methodologies that enhance the visualization, analysis, and interpretation of biological structures and processes with unprecedented precision. In this context, fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerful technique that enables quantitative and concentration-independent imaging by measuring the fluorescence decay time of molecular probes. Unlike intensity-based fluorescence methods, FLIM provides robust data on molecular interactions, environments, and biomarker presence, making it invaluable for bioimaging. However, its widespread adoption remains constrained by technical complexity, the high cost of instrumentation, and the underdevelopment of fluorescence lifetime probes with AIE characteristics. A promising solution to these challenges lies in aggregation-induced emission (AIE) fluorophores, which overcome issues such as aggregation-caused quenching and photobleaching. Despite their potential, AIE fluorophores have been rarely integrated with FLIM, leaving a significant gap in bioimaging research. Integrating FLIM with AIE probes could revolutionize bioimaging, enabling precise, stable, and environment-sensitive lifetime imaging for diagnostics, metabolism, and live-cell monitoring. In this perspective, we examine the potential of FLIM-AIE integration in bioimaging, highlighting its advantages, identifying key barriers, and proposing strategies to promote its use in biomedical research. We highlight the critical role of interdisciplinary collaboration, enhanced training programs, and targeted funding initiatives in accelerating innovation in the field of bioimaging. Main textElsevier202620262025info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://doi.org/10.1016/j.xinn.2025.101081https://hdl.handle.net/10578/46145reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésRED2022-134287-TCPP2021-008597SBPLY/21/180501/000050SBPLY/23/180502/000013Predoctoral grant 2024-PRED-21958info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/461452026-05-27T07:36:41Z
dc.title.none.fl_str_mv Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
spellingShingle Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
Blas Gómez, Sofía
Bioimaging
title_short Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_full Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_fullStr Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_full_unstemmed Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_sort Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
dc.creator.none.fl_str_mv Blas Gómez, Sofía
Alonso Moreno, Carlos
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author Blas Gómez, Sofía
author_facet Blas Gómez, Sofía
Alonso Moreno, Carlos
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author_role author
author2 Alonso Moreno, Carlos
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author2_role author
author
author
dc.subject.none.fl_str_mv Bioimaging
topic Bioimaging
description The frontiers of bioimaging encompass cutting-edge technologies and methodologies that enhance the visualization, analysis, and interpretation of biological structures and processes with unprecedented precision. In this context, fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerful technique that enables quantitative and concentration-independent imaging by measuring the fluorescence decay time of molecular probes. Unlike intensity-based fluorescence methods, FLIM provides robust data on molecular interactions, environments, and biomarker presence, making it invaluable for bioimaging. However, its widespread adoption remains constrained by technical complexity, the high cost of instrumentation, and the underdevelopment of fluorescence lifetime probes with AIE characteristics. A promising solution to these challenges lies in aggregation-induced emission (AIE) fluorophores, which overcome issues such as aggregation-caused quenching and photobleaching. Despite their potential, AIE fluorophores have been rarely integrated with FLIM, leaving a significant gap in bioimaging research. Integrating FLIM with AIE probes could revolutionize bioimaging, enabling precise, stable, and environment-sensitive lifetime imaging for diagnostics, metabolism, and live-cell monitoring. In this perspective, we examine the potential of FLIM-AIE integration in bioimaging, highlighting its advantages, identifying key barriers, and proposing strategies to promote its use in biomedical research. We highlight the critical role of interdisciplinary collaboration, enhanced training programs, and targeted funding initiatives in accelerating innovation in the field of bioimaging. Main text
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.xinn.2025.101081
https://hdl.handle.net/10578/46145
url https://doi.org/10.1016/j.xinn.2025.101081
https://hdl.handle.net/10578/46145
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv RED2022-134287-T
CPP2021-008597
SBPLY/21/180501/000050
SBPLY/23/180502/000013
Predoctoral grant 2024-PRED-21958
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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