Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing

Objective: Numerous studies have compared ovarian tissue cryopreservation methods, including slow freezing/ rapid thawing (SF/RT) and vitrification/warming (V/W), focusing on morpho-functional status, follicle density, stromal cell integrity, and gene expression during in vitro culture. However, res...

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Autores: Méndez M, Herranz-Diez C, González S, Ferreri J, Calafell JM, Otero J, Farre R, Civico S, Fabregues F
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p29261
Acesso em linha:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29261
Access Level:acceso abierto
Palavra-chave:ovarian cryopreservation
vitrification
slow freezing
mechanobiology
tissue stiffness
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spelling Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezingMéndez MHerranz-Diez CGonzález SFerreri JCalafell JMOtero JFarre RCivico SFabregues Fovarian cryopreservationvitrificationslow freezingmechanobiologytissue stiffnessObjective: Numerous studies have compared ovarian tissue cryopreservation methods, including slow freezing/ rapid thawing (SF/RT) and vitrification/warming (V/W), focusing on morpho-functional status, follicle density, stromal cell integrity, and gene expression during in vitro culture. However, results remain inconclusive. This study aims to evaluate the effects of SF/RT and V/W on the ovarian cortex's biomechanical properties. Methods: Human ovarian biopsies were taken from five women between 32 and 45 years undergoing laparoscopic surgery for tubal sterilization. For each patient, one small sample of fresh tissue was used as control, and the remaining tissue were vitrified or frozen by slow freezing method. The tissue stiffness of the cryopreserved samples at the micrometer scale was measured by Atomic Force Microscopy after thawing and warming, depending of the cryopreservation method. Results: The median stiffness of the ovarian cortex was 3670.00 Pa (Pascal) (IQR 2146.4) in the control group. After cryopreservation, the median stiffness slightly decreased to 1305.90 Pa (IQR 503.51) with SF/RT and to 2284.50 Pa (IQR 3314.40) with V/W. General linear model analysis revealed no significant effect of cryopreservation method on the ovarian cortex stiffness (F=2.750, p=0.071). No significant differences were observed based on the intrasample zone studied by AFM. However, a significant inter-patient effect on tissue stiffness was identified (F=3.958, p=0.006). Conclusions: The study findings suggest that ovarian tissue freezing methods do not have a relevant impact on functional aspects of the extracellular matrix (ECM), suggesting that given the logistical advantages of vitrification, this technique should be prioritized.SOC BRASILEIRA REPRODUCAO ASSISTIDA-SBRA2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29261Jornal Brasileiro de Reproducao AssistidaISSN: 15175693ISSNe: 15180557reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuPortuguésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p292612026-05-27T12:37:41Z
dc.title.none.fl_str_mv Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
title Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
spellingShingle Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
Méndez M
ovarian cryopreservation
vitrification
slow freezing
mechanobiology
tissue stiffness
title_short Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
title_full Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
title_fullStr Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
title_full_unstemmed Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
title_sort Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing
dc.creator.none.fl_str_mv Méndez M
Herranz-Diez C
González S
Ferreri J
Calafell JM
Otero J
Farre R
Civico S
Fabregues F
author Méndez M
author_facet Méndez M
Herranz-Diez C
González S
Ferreri J
Calafell JM
Otero J
Farre R
Civico S
Fabregues F
author_role author
author2 Herranz-Diez C
González S
Ferreri J
Calafell JM
Otero J
Farre R
Civico S
Fabregues F
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ovarian cryopreservation
vitrification
slow freezing
mechanobiology
tissue stiffness
topic ovarian cryopreservation
vitrification
slow freezing
mechanobiology
tissue stiffness
description Objective: Numerous studies have compared ovarian tissue cryopreservation methods, including slow freezing/ rapid thawing (SF/RT) and vitrification/warming (V/W), focusing on morpho-functional status, follicle density, stromal cell integrity, and gene expression during in vitro culture. However, results remain inconclusive. This study aims to evaluate the effects of SF/RT and V/W on the ovarian cortex's biomechanical properties. Methods: Human ovarian biopsies were taken from five women between 32 and 45 years undergoing laparoscopic surgery for tubal sterilization. For each patient, one small sample of fresh tissue was used as control, and the remaining tissue were vitrified or frozen by slow freezing method. The tissue stiffness of the cryopreserved samples at the micrometer scale was measured by Atomic Force Microscopy after thawing and warming, depending of the cryopreservation method. Results: The median stiffness of the ovarian cortex was 3670.00 Pa (Pascal) (IQR 2146.4) in the control group. After cryopreservation, the median stiffness slightly decreased to 1305.90 Pa (IQR 503.51) with SF/RT and to 2284.50 Pa (IQR 3314.40) with V/W. General linear model analysis revealed no significant effect of cryopreservation method on the ovarian cortex stiffness (F=2.750, p=0.071). No significant differences were observed based on the intrasample zone studied by AFM. However, a significant inter-patient effect on tissue stiffness was identified (F=3.958, p=0.006). Conclusions: The study findings suggest that ovarian tissue freezing methods do not have a relevant impact on functional aspects of the extracellular matrix (ECM), suggesting that given the logistical advantages of vitrification, this technique should be prioritized.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29261
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29261
dc.language.none.fl_str_mv Portugués
language_invalid_str_mv Portugués
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv SOC BRASILEIRA REPRODUCAO ASSISTIDA-SBRA
publisher.none.fl_str_mv SOC BRASILEIRA REPRODUCAO ASSISTIDA-SBRA
dc.source.none.fl_str_mv Jornal Brasileiro de Reproducao Assistida
ISSN: 15175693
ISSNe: 15180557
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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