Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates

The present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10min light, 10min rest and 10min of further light (10-10-1...

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Autores: Yeste Oliveras, Marc|||0000-0002-2209-340X, Codony, Francesc|||0000-0002-8679-5128, Estrada, Efrén|||0000-0002-0824-3651, Lleonart, Miquel, Balasch, Sam, Peña, Alejandro, Bonet, Sergi|||0000-0002-5125-7274, Rodríguez-Gil, Joan E.
Tipo de documento: artigo
Data de publicação:2016
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:205997
Acesso em linha:https://ddd.uab.cat/record/205997
https://dx.doi.org/urn:doi:10.1038/srep22569
Access Level:Acceso aberto
Palavra-chave:Esperma
Porcs senglars
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spelling Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculatesYeste Oliveras, Marc|||0000-0002-2209-340XCodony, Francesc|||0000-0002-8679-5128Estrada, Efrén|||0000-0002-0824-3651Lleonart, MiquelBalasch, SamPeña, AlejandroBonet, Sergi|||0000-0002-5125-7274Rodríguez-Gil, Joan E.EspermaPorcs senglarsThe present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10min light, 10min rest and 10min of further light (10-10-10 pattern). This pattern induced an intense and transient increase in the majority of motility parameters, without modifying sperm viability and acrosome integrity. While incubating non-photo-stimulated sperm at 37°C for 90min decreased all sperm quality parameters, this reduction was prevented when the previously-described light procedure was applied. This effect was concomitant with an increase in the percentage of sperm with high mitochondrial membrane potential. When sperm were subjected to 'in vitro' capacitation, photo-stimulation also increased the percentage of sperm with capacitation-like changes in membrane structure. On the other hand, treating commercial semen doses intended for artificial insemination with the 10-10-10 photo-stimulation pattern significantly increased farrowing rates and the number of both total and live-born piglets for parturition. Therefore, our results indicate that a precise photo-stimulation procedure is able to increase the fertilising ability of boar sperm via a mechanism that could be related to mitochondrial function. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/205997https://dx.doi.org/urn:doi:10.1038/srep22569reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2059972026-06-06T12:50:31Z
dc.title.none.fl_str_mv Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
title Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
spellingShingle Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
Yeste Oliveras, Marc|||0000-0002-2209-340X
Esperma
Porcs senglars
title_short Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
title_full Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
title_fullStr Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
title_full_unstemmed Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
title_sort Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
dc.creator.none.fl_str_mv Yeste Oliveras, Marc|||0000-0002-2209-340X
Codony, Francesc|||0000-0002-8679-5128
Estrada, Efrén|||0000-0002-0824-3651
Lleonart, Miquel
Balasch, Sam
Peña, Alejandro
Bonet, Sergi|||0000-0002-5125-7274
Rodríguez-Gil, Joan E.
author Yeste Oliveras, Marc|||0000-0002-2209-340X
author_facet Yeste Oliveras, Marc|||0000-0002-2209-340X
Codony, Francesc|||0000-0002-8679-5128
Estrada, Efrén|||0000-0002-0824-3651
Lleonart, Miquel
Balasch, Sam
Peña, Alejandro
Bonet, Sergi|||0000-0002-5125-7274
Rodríguez-Gil, Joan E.
author_role author
author2 Codony, Francesc|||0000-0002-8679-5128
Estrada, Efrén|||0000-0002-0824-3651
Lleonart, Miquel
Balasch, Sam
Peña, Alejandro
Bonet, Sergi|||0000-0002-5125-7274
Rodríguez-Gil, Joan E.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Esperma
Porcs senglars
topic Esperma
Porcs senglars
description The present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10min light, 10min rest and 10min of further light (10-10-10 pattern). This pattern induced an intense and transient increase in the majority of motility parameters, without modifying sperm viability and acrosome integrity. While incubating non-photo-stimulated sperm at 37°C for 90min decreased all sperm quality parameters, this reduction was prevented when the previously-described light procedure was applied. This effect was concomitant with an increase in the percentage of sperm with high mitochondrial membrane potential. When sperm were subjected to 'in vitro' capacitation, photo-stimulation also increased the percentage of sperm with capacitation-like changes in membrane structure. On the other hand, treating commercial semen doses intended for artificial insemination with the 10-10-10 photo-stimulation pattern significantly increased farrowing rates and the number of both total and live-born piglets for parturition. Therefore, our results indicate that a precise photo-stimulation procedure is able to increase the fertilising ability of boar sperm via a mechanism that could be related to mitochondrial function.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/205997
https://dx.doi.org/urn:doi:10.1038/srep22569
url https://ddd.uab.cat/record/205997
https://dx.doi.org/urn:doi:10.1038/srep22569
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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