In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration

This study evaluated the biological performance in vitro of two 3D-printed hydroxyapatite (HA) and polylactic acid (PLA) composite scaffolds with two different infill densities (50% [HA-PLA50] and 70% [HA-PLA70]). Comparative analysis using MG-63 cell cultures evaluated the following: (1) integrity...

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Autores: Shan, Eddy, Chamorro, Cristina, Ferrández-Montero, Ana, Martin Rodríguez, Rosa M., Ferrari, Begoña, Sánchez-Herencia, A. Javier, Virto, Leire, Marín, María José, Figuero, Elena, Sanz, Mariano
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394155
Acceso en línea:http://hdl.handle.net/10261/394155
Access Level:acceso abierto
Palabra clave:Tissue engineering
Hydroxyapatite-polylactic acid
In vitro
Scaffold
Additive manufacturing
Bone regeneration
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spelling In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone RegenerationShan, EddyChamorro, CristinaFerrández-Montero, AnaMartin Rodríguez, Rosa M.Ferrari, BegoñaSánchez-Herencia, A. JavierVirto, LeireMarín, María JoséFiguero, ElenaSanz, MarianoTissue engineeringHydroxyapatite-polylactic acidIn vitroScaffoldAdditive manufacturingBone regenerationThis study evaluated the biological performance in vitro of two 3D-printed hydroxyapatite (HA) and polylactic acid (PLA) composite scaffolds with two different infill densities (50% [HA-PLA50] and 70% [HA-PLA70]). Comparative analysis using MG-63 cell cultures evaluated the following: (1) integrity after exposure to various sterilization methods; (2) cell viability; (3) morphological characteristics; (4) cell proliferation; (5) cytotoxicity; (6) gene expression; and (7) protein synthesis. Ultraviolet radiation was the preferred sterilization method. Both scaffolds maintained adequate cell viability and proliferation over 7 days without significant differences in cytotoxicity. Notably, HA-PLA50 scaffolds demonstrated superior osteogenic potential, showing a significantly higher expression of collagen type I (COL1A1) and an increased synthesis of interleukins 6 and 8 (IL-6, IL-8) compared to HA-PLA70 scaffolds. While both scaffold types supported robust cell growth, the HA-PLA50 formulation exhibited enhanced bioactivity, suggesting a potential advantage for bone tissue engineering applications. These findings provide important insights for optimizing 3D-printed bone graft substitutes.This research was supported by the Spanish Centre for the Development of Industrial Technology (CDTI) through the grant SNEO-20211395 to COLFEED4Print S.L. which contracted the Complutense University of Madrid. A. Ferrandez-Montero is granted by the Comunidad de Madrid for “Atracción de Talento” project 2022-T1/IND-23973 and PID2022-137274NB-C31 (3DPOSTPERFORM) funded by MICIU/AEI/10.13039/501100011033 and by ERDF/EU.Peer reviewedMultidisciplinary Digital Publishing InstituteCentro para el Desarrollo Tecnológico y la Innovación (España)Comunidad de MadridMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/394155reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#2022-T1/IND-23973/Atracción de Talentoinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137274NB-C31The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/jfb16060218https://doi.org/10.3390/jfb16060218Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3941552026-05-22T06:33:51Z
dc.title.none.fl_str_mv In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
title In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
spellingShingle In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
Shan, Eddy
Tissue engineering
Hydroxyapatite-polylactic acid
In vitro
Scaffold
Additive manufacturing
Bone regeneration
title_short In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
title_full In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
title_fullStr In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
title_full_unstemmed In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
title_sort In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration
dc.creator.none.fl_str_mv Shan, Eddy
Chamorro, Cristina
Ferrández-Montero, Ana
Martin Rodríguez, Rosa M.
Ferrari, Begoña
Sánchez-Herencia, A. Javier
Virto, Leire
Marín, María José
Figuero, Elena
Sanz, Mariano
author Shan, Eddy
author_facet Shan, Eddy
Chamorro, Cristina
Ferrández-Montero, Ana
Martin Rodríguez, Rosa M.
Ferrari, Begoña
Sánchez-Herencia, A. Javier
Virto, Leire
Marín, María José
Figuero, Elena
Sanz, Mariano
author_role author
author2 Chamorro, Cristina
Ferrández-Montero, Ana
Martin Rodríguez, Rosa M.
Ferrari, Begoña
Sánchez-Herencia, A. Javier
Virto, Leire
Marín, María José
Figuero, Elena
Sanz, Mariano
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro para el Desarrollo Tecnológico y la Innovación (España)
Comunidad de Madrid
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Tissue engineering
Hydroxyapatite-polylactic acid
In vitro
Scaffold
Additive manufacturing
Bone regeneration
topic Tissue engineering
Hydroxyapatite-polylactic acid
In vitro
Scaffold
Additive manufacturing
Bone regeneration
description This study evaluated the biological performance in vitro of two 3D-printed hydroxyapatite (HA) and polylactic acid (PLA) composite scaffolds with two different infill densities (50% [HA-PLA50] and 70% [HA-PLA70]). Comparative analysis using MG-63 cell cultures evaluated the following: (1) integrity after exposure to various sterilization methods; (2) cell viability; (3) morphological characteristics; (4) cell proliferation; (5) cytotoxicity; (6) gene expression; and (7) protein synthesis. Ultraviolet radiation was the preferred sterilization method. Both scaffolds maintained adequate cell viability and proliferation over 7 days without significant differences in cytotoxicity. Notably, HA-PLA50 scaffolds demonstrated superior osteogenic potential, showing a significantly higher expression of collagen type I (COL1A1) and an increased synthesis of interleukins 6 and 8 (IL-6, IL-8) compared to HA-PLA70 scaffolds. While both scaffold types supported robust cell growth, the HA-PLA50 formulation exhibited enhanced bioactivity, suggesting a potential advantage for bone tissue engineering applications. These findings provide important insights for optimizing 3D-printed bone graft substitutes.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394155
url http://hdl.handle.net/10261/394155
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
2022-T1/IND-23973/Atracción de Talento
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137274NB-C31
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/jfb16060218
https://doi.org/10.3390/jfb16060218
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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