Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits

16 páginas, 1 tabla, 3 figuras.

Detalles Bibliográficos
Autores: Vallejo, R., Benavides, Julio, Arteche-Villasol, Noive, Fernández-Escobar, M., Ferreras, Mª del Carmen, Pérez Pérez, Valentín, Gutiérrez-Expósito, Daniel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/287056
Acceso en línea:http://hdl.handle.net/10261/287056
Access Level:acceso abierto
Palabra clave:Toxoplasma gondii
Sheep
Genotype
Macrophages
Internalization
Citokines
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spelling Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traitsVallejo, R.Benavides, JulioArteche-Villasol, NoiveFernández-Escobar, M.Ferreras, Mª del CarmenPérez Pérez, ValentínGutiérrez-Expósito, DanielToxoplasma gondiiSheepGenotypeMacrophagesInternalizationCitokines16 páginas, 1 tabla, 3 figuras.Ovine toxoplasmosis is one the most relevant reproductive diseases in sheep. The genetic variability among different Toxoplasma gondii isolates is known to be related to different degrees of virulence in mice and humans, but little is known regarding its potential effects in sheep. The aim of this study was to investigate the effect of genetic variability (types II (ToxoDB #1 and #3) and III (#2)) of six recently isolated strains that showed different phenotypic traits both in a normalized mouse model and in ovine trophoblasts, in ovine monocyte-derived macrophages and the subsequent transcript expression of cytokines and iNOS (inducible nitric oxide synthase). The type III isolate (TgShSp24) showed the highest rate of internalization, followed by the type II clonal isolate (TgShSp2), while the type II PRU isolates (TgShSp1, TgShSp3, TgShSp11 and TgShSp16) showed the lowest rates. The type II PRU strains, isolated from abortions, exhibited higher levels of anti-inflammatory cytokines and iNOS than those obtained from the myocardium of chronically infected sheep (type II PRU strains and type III), which had higher levels of pro-inflammatory cytokines. The present results show the existence of significant intra- and inter-genotypic differences in the parasite-macrophage relationship that need to be confirmed in in vivo experiments.This research was funded by projects of the “Junta de Castilla y León” (LE080U16) and the Spanish Ministry of Sciences (AGL2016-75935-C2-1-R and AGL2016-75935-C2-2-R). R.V. and N.A.-V. were recipients of a predoctoral contract from the Ministry of Science and Innovation (grant nos. FPU-17/05346 and BES-2016076513, respectively).Peer reviewedMultidisciplinary Digital Publishing InstituteJunta de Castilla y LeónMinisterio de Ciencia e Innovación (España)Benavides, Julio [0000-0001-9706-100X]Arteche Villasol, Noive [0000-0001-5793-9578]Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]Pérez Pérez, Valentín [0000-0003-0075-1587]Gutiérrez Expósito, Daniel [0000-0002-7683-623X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/287056reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MCINN//BES-2016076513info:eu-repo/grantAgreement/MINECO//AGL2016-75935-C2-1-Rinfo:eu-repo/grantAgreement/MINECO//AGL2016-75935-C2-2-Rhttps://dx.doi.org/10.3390/ani12243453Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2870562026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
title Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
spellingShingle Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
Vallejo, R.
Toxoplasma gondii
Sheep
Genotype
Macrophages
Internalization
Citokines
title_short Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
title_full Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
title_fullStr Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
title_full_unstemmed Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
title_sort Effects of ovine monocyte-derived macrophage infection by recently isolated Toxoplasma gondii strains showing different phenotypic traits
dc.creator.none.fl_str_mv Vallejo, R.
Benavides, Julio
Arteche-Villasol, Noive
Fernández-Escobar, M.
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
Gutiérrez-Expósito, Daniel
author Vallejo, R.
author_facet Vallejo, R.
Benavides, Julio
Arteche-Villasol, Noive
Fernández-Escobar, M.
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
Gutiérrez-Expósito, Daniel
author_role author
author2 Benavides, Julio
Arteche-Villasol, Noive
Fernández-Escobar, M.
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
Gutiérrez-Expósito, Daniel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Castilla y León
Ministerio de Ciencia e Innovación (España)
Benavides, Julio [0000-0001-9706-100X]
Arteche Villasol, Noive [0000-0001-5793-9578]
Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229]
Pérez Pérez, Valentín [0000-0003-0075-1587]
Gutiérrez Expósito, Daniel [0000-0002-7683-623X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Toxoplasma gondii
Sheep
Genotype
Macrophages
Internalization
Citokines
topic Toxoplasma gondii
Sheep
Genotype
Macrophages
Internalization
Citokines
description 16 páginas, 1 tabla, 3 figuras.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/287056
url http://hdl.handle.net/10261/287056
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MCINN//BES-2016076513
info:eu-repo/grantAgreement/MINECO//AGL2016-75935-C2-1-R
info:eu-repo/grantAgreement/MINECO//AGL2016-75935-C2-2-R
https://dx.doi.org/10.3390/ani12243453
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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