Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection

Primary infection of legumes by rhizobia involves the controlled localized enzymatic breakdown of cell walls at root hair tips. Previous studies determined the role of rhizobial CelC2 cellulase in different steps of the symbiotic interaction Rhizobium leguminosarum-Trifolium repens. Recent findings...

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Autores: Menéndez, E., Robledo Garrido, Marta, Jiménez Zurdo, J.I., Velázquez, E., Rivas, R., Murray, J.D., Mateos, P. F.
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/36118
Acceso en línea:https://hdl.handle.net/10902/36118
Access Level:acceso abierto
Palabra clave:Cellulase CelC2
Rhizobium
Trifolium
Medicago
Hot phenotype
Ensifer meliloti
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spelling Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infectionMenéndez, E.Robledo Garrido, MartaJiménez Zurdo, J.I.Velázquez, E.Rivas, R.Murray, J.D.Mateos, P. F.Cellulase CelC2RhizobiumTrifoliumMedicagoHot phenotypeEnsifer melilotiPrimary infection of legumes by rhizobia involves the controlled localized enzymatic breakdown of cell walls at root hair tips. Previous studies determined the role of rhizobial CelC2 cellulase in different steps of the symbiotic interaction Rhizobium leguminosarum-Trifolium repens. Recent findings also showed that CelC2 influences early signalling events in the Ensifer meliloti-Medicago truncatula interaction. Here, we have monitored the root hair phenotypes of two legume plants, T. repens and M. sativa, upon inoculation with strains of their cognate and non-cognate rhizobial species, R. leguminosarum bv trifolii and E. meliloti, (over)expressing the CelC2 coding gene, celC. Regardless of the host, CelC2 specifically elicited 'hole-on-the-tip' events (Hot phenotype) in the root hair apex, consistent with the role of this endoglucanase in eroding the noncrystalline cellulose found in polarly growing cell walls. Overproduction of CelC2 also increased root hair tip redirections (RaT phenotype) events in both cognate and non-cognate hosts. Interestingly, heterologous celC expression also induced non-canonical alterations in ROS (Reactive Oxygen Species) homeostasis at root hair tips of Trifolium and Medicago. These results suggest the concurrence of shared unspecific and host-related plant responses to CelC2 during early steps of symbiotic rhizobial infection. Our data thus identify CelC2 cellulase as an important determinant of events underlying early infection of the legume host by rhizobia.Acknowledgments: This work was supported by Grant AGL2015-70510-R from MINECO (Spanish Ministry of Economy, Industry and Competitiveness). E.M. and M.R. were supported by PhD fellowships from the Spanish government. We acknowledge Andrew Breakspear for his help with root hairs RNA extraction and Chengwu Liu for his help with NBT staining and microscopy visualization. Tis paper is dedicated to Professor Frank B. Dazzo on the occasion of his retirement.Nature Publishing GroupUniversidad de Cantabria20192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttps://hdl.handle.net/10902/36118Scientific Reports, 2019, 9(1), 13907reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/361182026-06-02T12:39:31Z
dc.title.none.fl_str_mv Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
title Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
spellingShingle Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
Menéndez, E.
Cellulase CelC2
Rhizobium
Trifolium
Medicago
Hot phenotype
Ensifer meliloti
title_short Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
title_full Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
title_fullStr Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
title_full_unstemmed Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
title_sort Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
dc.creator.none.fl_str_mv Menéndez, E.
Robledo Garrido, Marta
Jiménez Zurdo, J.I.
Velázquez, E.
Rivas, R.
Murray, J.D.
Mateos, P. F.
author Menéndez, E.
author_facet Menéndez, E.
Robledo Garrido, Marta
Jiménez Zurdo, J.I.
Velázquez, E.
Rivas, R.
Murray, J.D.
Mateos, P. F.
author_role author
author2 Robledo Garrido, Marta
Jiménez Zurdo, J.I.
Velázquez, E.
Rivas, R.
Murray, J.D.
Mateos, P. F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Cellulase CelC2
Rhizobium
Trifolium
Medicago
Hot phenotype
Ensifer meliloti
topic Cellulase CelC2
Rhizobium
Trifolium
Medicago
Hot phenotype
Ensifer meliloti
description Primary infection of legumes by rhizobia involves the controlled localized enzymatic breakdown of cell walls at root hair tips. Previous studies determined the role of rhizobial CelC2 cellulase in different steps of the symbiotic interaction Rhizobium leguminosarum-Trifolium repens. Recent findings also showed that CelC2 influences early signalling events in the Ensifer meliloti-Medicago truncatula interaction. Here, we have monitored the root hair phenotypes of two legume plants, T. repens and M. sativa, upon inoculation with strains of their cognate and non-cognate rhizobial species, R. leguminosarum bv trifolii and E. meliloti, (over)expressing the CelC2 coding gene, celC. Regardless of the host, CelC2 specifically elicited 'hole-on-the-tip' events (Hot phenotype) in the root hair apex, consistent with the role of this endoglucanase in eroding the noncrystalline cellulose found in polarly growing cell walls. Overproduction of CelC2 also increased root hair tip redirections (RaT phenotype) events in both cognate and non-cognate hosts. Interestingly, heterologous celC expression also induced non-canonical alterations in ROS (Reactive Oxygen Species) homeostasis at root hair tips of Trifolium and Medicago. These results suggest the concurrence of shared unspecific and host-related plant responses to CelC2 during early steps of symbiotic rhizobial infection. Our data thus identify CelC2 cellulase as an important determinant of events underlying early infection of the legume host by rhizobia.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10902/36118
url https://hdl.handle.net/10902/36118
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific Reports, 2019, 9(1), 13907
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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