Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake

Purpose Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogene...

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Autores: Coto, Laura, Sousa Martín, Carolina, Cebolla, Ángel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/131289
Acceso en línea:https://hdl.handle.net/11441/131289
https://doi.org/10.1007/s00394-021-02765-z
Access Level:acceso abierto
Palabra clave:Gluten immunogenic peptides
Gluten metabolism
Gluten detection feces
Gluten-free diet monitoring
Celiac disease
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spelling Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intakeCoto, LauraSousa Martín, CarolinaCebolla, ÁngelGluten immunogenic peptidesGluten metabolismGluten detection fecesGluten-free diet monitoringCeliac diseasePurpose Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogeneously controlled dietary conditions, and the sensitivity of fecal GIP tests after low amounts of punctual gluten ingestions. Methods Participants (n = 20) followed the same gluten-free menu for 12 days in which two separated doses of gluten (50 mg and 2 g) were ingested and all the depositions were collected. GIP from stool samples were analyzed by ELISA and lateral flow immunoassay (LFIA) tests. Results Most participants had detectable GIP after 50 mg and 2 g gluten ingestions using ELISA test (72.2% and 95%, respectively), whereas the LFIA test showed less sensitivity (22.2% and 80%, respectively). GIP were detected at higher either frequency or concentration in the range of 12–36 h after 50 mg intake, and 12–84 h after 2 g consumption. Considering this period, diagnostic sensitivity of GIP detection after a single 50 mg ingestion may be significatively increased analyzing three stool samples per individual. High variability among participants was found in the time and amount of GIP excretion; however, some individuals showed common patterns for both gluten intakes. Conclusion Sporadic gluten exposure detection may require several fecal samples to achieve level of sensitivity above 90%. Interindividual variability in the dynamic of GIP excretion may suggest patterns of gluten metabolism.España Ministerio de Ciencia e Innovación (DI-16-08943)España Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad and FEDER funds (AT17_5489_USE), Centro para el Desarrollo Tecnológico Industrial (IDI-20180051) and Corporación Tecnológica de Andalucía (17/957).SpringerMicrobiología y ParasitologíaMinisterio de Ciencia e Innovación (MICIN). EspañaJunta de Andalucía2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/131289https://doi.org/10.1007/s00394-021-02765-zreponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésEuropean Journal of Nutrition, 1-17.DI-16-08943AT17_5489_USEIDI-20180051http://dx.doi.org/10.1007/s00394-021-02765-zinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1312892026-06-17T12:51:07Z
dc.title.none.fl_str_mv Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
spellingShingle Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
Coto, Laura
Gluten immunogenic peptides
Gluten metabolism
Gluten detection feces
Gluten-free diet monitoring
Celiac disease
title_short Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_full Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_fullStr Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_full_unstemmed Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_sort Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
dc.creator.none.fl_str_mv Coto, Laura
Sousa Martín, Carolina
Cebolla, Ángel
author Coto, Laura
author_facet Coto, Laura
Sousa Martín, Carolina
Cebolla, Ángel
author_role author
author2 Sousa Martín, Carolina
Cebolla, Ángel
author2_role author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
dc.subject.none.fl_str_mv Gluten immunogenic peptides
Gluten metabolism
Gluten detection feces
Gluten-free diet monitoring
Celiac disease
topic Gluten immunogenic peptides
Gluten metabolism
Gluten detection feces
Gluten-free diet monitoring
Celiac disease
description Purpose Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogeneously controlled dietary conditions, and the sensitivity of fecal GIP tests after low amounts of punctual gluten ingestions. Methods Participants (n = 20) followed the same gluten-free menu for 12 days in which two separated doses of gluten (50 mg and 2 g) were ingested and all the depositions were collected. GIP from stool samples were analyzed by ELISA and lateral flow immunoassay (LFIA) tests. Results Most participants had detectable GIP after 50 mg and 2 g gluten ingestions using ELISA test (72.2% and 95%, respectively), whereas the LFIA test showed less sensitivity (22.2% and 80%, respectively). GIP were detected at higher either frequency or concentration in the range of 12–36 h after 50 mg intake, and 12–84 h after 2 g consumption. Considering this period, diagnostic sensitivity of GIP detection after a single 50 mg ingestion may be significatively increased analyzing three stool samples per individual. High variability among participants was found in the time and amount of GIP excretion; however, some individuals showed common patterns for both gluten intakes. Conclusion Sporadic gluten exposure detection may require several fecal samples to achieve level of sensitivity above 90%. Interindividual variability in the dynamic of GIP excretion may suggest patterns of gluten metabolism.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/131289
https://doi.org/10.1007/s00394-021-02765-z
url https://hdl.handle.net/11441/131289
https://doi.org/10.1007/s00394-021-02765-z
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv European Journal of Nutrition, 1-17.
DI-16-08943
AT17_5489_USE
IDI-20180051
http://dx.doi.org/10.1007/s00394-021-02765-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
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