Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks

Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected fr...

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Autores: Fernández de Mera, Isabel G., Chaligiannis, Ilias, Hernández-Jarguín, Angélica, Villar, Margarita, Mateos-Hernández, Lourdes, Papa, Anna, Sotiraki, Smaragda, Ruiz-Fons, Francisco, Cabezas-Cruz, Alejandro, Gortázar, Christian, Fuente, José de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/173852
Acceso en línea:http://hdl.handle.net/10261/173852
Access Level:acceso abierto
Palabra clave:Infectious diseases
Genetics
Evolution
Virology
Public health
Veterinary science
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticksFernández de Mera, Isabel G.Chaligiannis, IliasHernández-Jarguín, AngélicaVillar, MargaritaMateos-Hernández, LourdesPapa, AnnaSotiraki, SmaragdaRuiz-Fons, FranciscoCabezas-Cruz, AlejandroGortázar, ChristianFuente, José de laInfectious diseasesGeneticsEvolutionVirologyPublic healthVeterinary sciencehttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesCrimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RT-PCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterization of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.This work was partially funded by the EU FP7 grant ANTIGONE (#278976) and State Scholarship Foundation IKY, NSRF 2007-2013. IGFM and MV were supported by the Research Plan of the University of Castilla-La Mancha (UCLM), Spain. LMH was supported by a fellowship from the UCLM.Peer ReviewedElsevierEuropean CommissionUniversidad de Castilla La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920172019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/173852reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/278976https://doi.org/10.1016/j.heliyon.2017.e00353Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1738522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
spellingShingle Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
Fernández de Mera, Isabel G.
Infectious diseases
Genetics
Evolution
Virology
Public health
Veterinary science
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_full Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_fullStr Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_full_unstemmed Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_sort Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
dc.creator.none.fl_str_mv Fernández de Mera, Isabel G.
Chaligiannis, Ilias
Hernández-Jarguín, Angélica
Villar, Margarita
Mateos-Hernández, Lourdes
Papa, Anna
Sotiraki, Smaragda
Ruiz-Fons, Francisco
Cabezas-Cruz, Alejandro
Gortázar, Christian
Fuente, José de la
author Fernández de Mera, Isabel G.
author_facet Fernández de Mera, Isabel G.
Chaligiannis, Ilias
Hernández-Jarguín, Angélica
Villar, Margarita
Mateos-Hernández, Lourdes
Papa, Anna
Sotiraki, Smaragda
Ruiz-Fons, Francisco
Cabezas-Cruz, Alejandro
Gortázar, Christian
Fuente, José de la
author_role author
author2 Chaligiannis, Ilias
Hernández-Jarguín, Angélica
Villar, Margarita
Mateos-Hernández, Lourdes
Papa, Anna
Sotiraki, Smaragda
Ruiz-Fons, Francisco
Cabezas-Cruz, Alejandro
Gortázar, Christian
Fuente, José de la
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Universidad de Castilla La Mancha
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Infectious diseases
Genetics
Evolution
Virology
Public health
Veterinary science
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic Infectious diseases
Genetics
Evolution
Virology
Public health
Veterinary science
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RT-PCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterization of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/173852
url http://hdl.handle.net/10261/173852
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/278976
https://doi.org/10.1016/j.heliyon.2017.e00353
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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