Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release

Developing prolonged antigen delivery systems that mimic long-term exposure to pathogens appears as a promising but still poorly explored approach to reach durable immunities. In this study, we have used a simple technology by which His-tagged proteins can be assembled, assisted by divalent cations,...

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Authors: Bosch Camós, Laia|||0000-0001-6245-4284, Martinez Torro, Carlos|||0000-0002-3529-3355, López-Laguna, Hèctor|||0000-0001-5249-8304, Lascorz Lozano, Jara A.|||0000-0002-5823-0728, Argilaguet, Jordi|||0000-0001-5924-2774, Villaverde, Antonio|||0000-0002-2615-4521, Rodriguez, Fernando|||0000-0001-7361-8408, Vázquez, Esther|||0000-0003-1052-0424
Format: article
Publication Date:2024
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:290350
Online Access:https://ddd.uab.cat/record/290350
https://dx.doi.org/urn:doi:10.3390/nano14050435
Access Level:Open access
Keyword:Recombinant proteins
Biomaterials
Protein nanoparticles
Secretory granules
Immunization
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spelling Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen ReleaseBosch Camós, Laia|||0000-0001-6245-4284Martinez Torro, Carlos|||0000-0002-3529-3355López-Laguna, Hèctor|||0000-0001-5249-8304Lascorz Lozano, Jara A.|||0000-0002-5823-0728Argilaguet, Jordi|||0000-0001-5924-2774Villaverde, Antonio|||0000-0002-2615-4521Rodriguez, Fernando|||0000-0001-7361-8408Vázquez, Esther|||0000-0003-1052-0424Recombinant proteinsBiomaterialsProtein nanoparticlesSecretory granulesImmunizationDeveloping prolonged antigen delivery systems that mimic long-term exposure to pathogens appears as a promising but still poorly explored approach to reach durable immunities. In this study, we have used a simple technology by which His-tagged proteins can be assembled, assisted by divalent cations, as supramolecular complexes with progressive complexity, namely protein-only nanoparticles and microparticles. Microparticles produced out of nanoparticles are biomimetics of secretory granules from the mammalian hormonal system. Upon subcutaneous administration, they slowly disintegrate, acting as an endocrine-like secretory system and rendering the building block nanoparticles progressively bioavailable. The performance of such materials, previously validated for drug delivery in oncology, has been tested here regarding the potential for time-prolonged antigen release. This has been completed by taking, as a building block, a nanostructured version of p30, a main structural immunogen from the African swine fever virus (ASFV). By challenging the system in both mice and pigs, we have observed unusually potent pro-inflammatory activity in porcine macrophages, and long-lasting humoral and cellular responses in vivo, which might overcome the need for an adjuvant. The robustness of both innate and adaptive responses tag, for the first time, these dynamic depot materials as a novel and valuable instrument with transversal applicability in immune stimulation and vaccinology. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/290350https://dx.doi.org/urn:doi:10.3390/nano14050435reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-105416RB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PDC2022-133858-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116174RB-I00Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00092Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2022-136312OB-I00Ministerio de Sanidad y Consumo CB06/01/0014open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2903502026-06-06T12:50:31Z
dc.title.none.fl_str_mv Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
title Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
spellingShingle Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
Bosch Camós, Laia|||0000-0001-6245-4284
Recombinant proteins
Biomaterials
Protein nanoparticles
Secretory granules
Immunization
title_short Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
title_full Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
title_fullStr Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
title_full_unstemmed Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
title_sort Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
dc.creator.none.fl_str_mv Bosch Camós, Laia|||0000-0001-6245-4284
Martinez Torro, Carlos|||0000-0002-3529-3355
López-Laguna, Hèctor|||0000-0001-5249-8304
Lascorz Lozano, Jara A.|||0000-0002-5823-0728
Argilaguet, Jordi|||0000-0001-5924-2774
Villaverde, Antonio|||0000-0002-2615-4521
Rodriguez, Fernando|||0000-0001-7361-8408
Vázquez, Esther|||0000-0003-1052-0424
author Bosch Camós, Laia|||0000-0001-6245-4284
author_facet Bosch Camós, Laia|||0000-0001-6245-4284
Martinez Torro, Carlos|||0000-0002-3529-3355
López-Laguna, Hèctor|||0000-0001-5249-8304
Lascorz Lozano, Jara A.|||0000-0002-5823-0728
Argilaguet, Jordi|||0000-0001-5924-2774
Villaverde, Antonio|||0000-0002-2615-4521
Rodriguez, Fernando|||0000-0001-7361-8408
Vázquez, Esther|||0000-0003-1052-0424
author_role author
author2 Martinez Torro, Carlos|||0000-0002-3529-3355
López-Laguna, Hèctor|||0000-0001-5249-8304
Lascorz Lozano, Jara A.|||0000-0002-5823-0728
Argilaguet, Jordi|||0000-0001-5924-2774
Villaverde, Antonio|||0000-0002-2615-4521
Rodriguez, Fernando|||0000-0001-7361-8408
Vázquez, Esther|||0000-0003-1052-0424
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Recombinant proteins
Biomaterials
Protein nanoparticles
Secretory granules
Immunization
topic Recombinant proteins
Biomaterials
Protein nanoparticles
Secretory granules
Immunization
description Developing prolonged antigen delivery systems that mimic long-term exposure to pathogens appears as a promising but still poorly explored approach to reach durable immunities. In this study, we have used a simple technology by which His-tagged proteins can be assembled, assisted by divalent cations, as supramolecular complexes with progressive complexity, namely protein-only nanoparticles and microparticles. Microparticles produced out of nanoparticles are biomimetics of secretory granules from the mammalian hormonal system. Upon subcutaneous administration, they slowly disintegrate, acting as an endocrine-like secretory system and rendering the building block nanoparticles progressively bioavailable. The performance of such materials, previously validated for drug delivery in oncology, has been tested here regarding the potential for time-prolonged antigen release. This has been completed by taking, as a building block, a nanostructured version of p30, a main structural immunogen from the African swine fever virus (ASFV). By challenging the system in both mice and pigs, we have observed unusually potent pro-inflammatory activity in porcine macrophages, and long-lasting humoral and cellular responses in vivo, which might overcome the need for an adjuvant. The robustness of both innate and adaptive responses tag, for the first time, these dynamic depot materials as a novel and valuable instrument with transversal applicability in immune stimulation and vaccinology.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/290350
https://dx.doi.org/urn:doi:10.3390/nano14050435
url https://ddd.uab.cat/record/290350
https://dx.doi.org/urn:doi:10.3390/nano14050435
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-105416RB-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PDC2022-133858-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116174RB-I00
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00092
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2022-136312OB-I00
Ministerio de Sanidad y Consumo CB06/01/0014
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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