Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry

Producción Científica

Detalhes bibliográficos
Autores: Bernal del Nozal, José, Nozal Nalda, María Jesús del, Martín Gómez, María Teresa, Bernal Yagüe, José Luis, Ares Sacristán, Ana María
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/63946
Acesso em linha:https://doi.org/10.1016/j.microc.2019.05.037
https://uvadoc.uva.es/handle/10324/63946
Access Level:acceso abierto
Palavra-chave:Bee pollen
Electrospray ionization mass spectrometry
EMR-lipid sorbent
Flubendiamide
Insecticides
Liquid chromatography
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spelling Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometryBernal del Nozal, JoséNozal Nalda, María Jesús delMartín Gómez, María TeresaBernal Yagüe, José LuisAres Sacristán, Ana MaríaBee pollenElectrospray ionization mass spectrometryEMR-lipid sorbentFlubendiamideInsecticidesLiquid chromatographyProducción CientíficaIn this work, a new method has been proposed with the aim of determining flubendiamide, a recently commercialized insecticide, in bee pollen by using liquid chromatography coupled to electrospray ionization mass spectrometry. For this purpose, a novel sample treatment has been proposed that has proven efficient in terms of recovery (average analyte recoveries were between 90% and 102%) and absence of matrix effect, and one which is effective, fast and selective. This involved a solvent extraction using an acetonitrile and water mixture, and a clean-up stage where, in addition to freezing, an enhanced matrix removal-lipid sorbent was successfully used for the first time with this matrix and analyte. The chromatographic conditions were also optimized, by selecting a C18 based column (Gemini® C18) and acetic acid (1 mM) in water and methanol as mobile phase components, allowing elution of flubendiamide in<4 min, with a total analysis time of 14 min. Validation was carried out, with the result that all the parameters studied complied with existing European legislation. It should be noted that the sensitivity of the method was excellent, with a quantification limit (4 μg/kg) well below the maximum residue level established for this insecticide in bee products (50 μg/kg). Finally, several bee pollen samples were analyzed, and flubendiamide residues were not found in any of the cases.Este trabajo forma parte de los proyectos de investigación financiados por el Ministerio de Economía y Competitividad e INIA-FEDER (RTA2013-00042-C10-03 y 06).Elsevier2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.microc.2019.05.037https://uvadoc.uva.es/handle/10324/63946reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/abs/pii/S0026265X19308422?via%3Dihubinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/639462026-06-13T12:44:47Z
dc.title.none.fl_str_mv Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
title Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
spellingShingle Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
Bernal del Nozal, José
Bee pollen
Electrospray ionization mass spectrometry
EMR-lipid sorbent
Flubendiamide
Insecticides
Liquid chromatography
title_short Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
title_full Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
title_fullStr Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
title_full_unstemmed Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
title_sort Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry
dc.creator.none.fl_str_mv Bernal del Nozal, José
Nozal Nalda, María Jesús del
Martín Gómez, María Teresa
Bernal Yagüe, José Luis
Ares Sacristán, Ana María
author Bernal del Nozal, José
author_facet Bernal del Nozal, José
Nozal Nalda, María Jesús del
Martín Gómez, María Teresa
Bernal Yagüe, José Luis
Ares Sacristán, Ana María
author_role author
author2 Nozal Nalda, María Jesús del
Martín Gómez, María Teresa
Bernal Yagüe, José Luis
Ares Sacristán, Ana María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bee pollen
Electrospray ionization mass spectrometry
EMR-lipid sorbent
Flubendiamide
Insecticides
Liquid chromatography
topic Bee pollen
Electrospray ionization mass spectrometry
EMR-lipid sorbent
Flubendiamide
Insecticides
Liquid chromatography
description Producción Científica
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.microc.2019.05.037
https://uvadoc.uva.es/handle/10324/63946
url https://doi.org/10.1016/j.microc.2019.05.037
https://uvadoc.uva.es/handle/10324/63946
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S0026265X19308422?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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