Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly

Cranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. To investigate the cellular behaviours involved in the 3D or...

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Detalles Bibliográficos
Autores: Bañón González, Aitor, Alsina i Español, Berta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/58521
Acceso en línea:http://hdl.handle.net/10230/58521
http://dx.doi.org/10.1242/dev.201824
Access Level:acceso abierto
Palabra clave:CRISPR
Delamination
Inner ear
Migration
Neuroblasts
RhoGTPases
Statoacoustic ganglion
Zebrafish
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oai_identifier_str oai:repositori.upf.edu:10230/58521
network_acronym_str ES
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repository_id_str
spelling Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assemblyBañón González, AitorAlsina i Español, BertaCRISPRDelaminationInner earMigrationNeuroblastsRhoGTPasesStatoacoustic ganglionZebrafishCranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation, and genetic perturbations. We show that otic NBs delaminate out of the otic epithelium in an epithelial-mesenchymal transition-like manner, rearranging apical polarity and primary cilia proteins. We also show that, once delaminated, NBs require RhoGTPases in order to perform active migration. Furthermore, tracking of recently delaminated NBs revealed their directed migration and coalescence around a small population of pioneer SAG neurons. These pioneer SAG neurons, not from otic placode origin, populate the coalescence region before otic neurogenesis begins and their ablation disrupts delaminated NB migratory pathways, consequentially affecting SAG shape. Altogether, this work shows for the first time the role of pioneer SAG neurons in orchestrating SAG development.Company Of Biologists Limited202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/58521http://dx.doi.org/10.1242/dev.201824reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésDevelopment. 2023 Nov 1;150(21):dev201824© 2023. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/585212026-06-12T07:21:37Z
dc.title.none.fl_str_mv Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
title Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
spellingShingle Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
Bañón González, Aitor
CRISPR
Delamination
Inner ear
Migration
Neuroblasts
RhoGTPases
Statoacoustic ganglion
Zebrafish
title_short Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
title_full Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
title_fullStr Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
title_full_unstemmed Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
title_sort Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
dc.creator.none.fl_str_mv Bañón González, Aitor
Alsina i Español, Berta
author Bañón González, Aitor
author_facet Bañón González, Aitor
Alsina i Español, Berta
author_role author
author2 Alsina i Español, Berta
author2_role author
dc.subject.none.fl_str_mv CRISPR
Delamination
Inner ear
Migration
Neuroblasts
RhoGTPases
Statoacoustic ganglion
Zebrafish
topic CRISPR
Delamination
Inner ear
Migration
Neuroblasts
RhoGTPases
Statoacoustic ganglion
Zebrafish
description Cranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation, and genetic perturbations. We show that otic NBs delaminate out of the otic epithelium in an epithelial-mesenchymal transition-like manner, rearranging apical polarity and primary cilia proteins. We also show that, once delaminated, NBs require RhoGTPases in order to perform active migration. Furthermore, tracking of recently delaminated NBs revealed their directed migration and coalescence around a small population of pioneer SAG neurons. These pioneer SAG neurons, not from otic placode origin, populate the coalescence region before otic neurogenesis begins and their ablation disrupts delaminated NB migratory pathways, consequentially affecting SAG shape. Altogether, this work shows for the first time the role of pioneer SAG neurons in orchestrating SAG development.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/58521
http://dx.doi.org/10.1242/dev.201824
url http://hdl.handle.net/10230/58521
http://dx.doi.org/10.1242/dev.201824
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Development. 2023 Nov 1;150(21):dev201824
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company Of Biologists Limited
publisher.none.fl_str_mv Company Of Biologists Limited
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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