Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites

© The Author(s).

Detalles Bibliográficos
Autores: Arévalo-Pinzón, Gabriela, González-González, María, Suárez, Carlos Fernando, Curtidor, Hernando, Carabias-Sánchez, Javier, Muro, Antonio, LaBaer, Joshua, Patarroyo, M. A., Fuentes, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/245363
Acceso en línea:http://hdl.handle.net/10261/245363
Access Level:acceso abierto
Palabra clave:Plasmodium vivax
Malaria
NAPPA array
IVTT protein expression
Protein–protein interaction
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spelling Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasitesArévalo-Pinzón, GabrielaGonzález-González, MaríaSuárez, Carlos FernandoCurtidor, HernandoCarabias-Sánchez, JavierMuro, AntonioLaBaer, JoshuaPatarroyo, M. A.Fuentes, ManuelPlasmodium vivaxMalariaNAPPA arrayIVTT protein expressionProtein–protein interaction© The Author(s).[Background]: Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein–protein and host–cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein–protein and host–protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification.[Results]: Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1.[Conclusions]: NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein–protein and ligand–receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax).This research was financed by the Colombian Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) through contract RC# 0309-2013 and the Spanish Institute of Health Carlos III (ISCIII) through Grant FIS PI 14/01538 & FIS PI 17/01930. We would also like to acknowledge contributions from the European Regional Development Fund (ERDF), the Junta Castilla-León (BIO/SA07/15) and Fundación Solórzano (FS-23-2015). The Proteomics Unit forms part of ProteoRed, PRB3-ISCIII, supported by Grant PT17/0019/0023, PE I+D+I 2017-2012, funded by ISCIII and ERDF. The Proteomics Unit belongs to ProteoRed, PRB3-ISCIII, supported by Grant PT17/0019/0023. The work was carried out during Arévalo-Pinzón’s PhD internship financed by COLCIENCIAS within the framework of the “Convocatoria Nacional para Estudios de Doctorado en Colombia (call for candidates no. 567)”.BioMed CentralColciencias (Colombia)Instituto de Salud Carlos IIIJunta de Castilla y LeónFundación Memoria de D. Samuel Solorzano BarrusoConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120182021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/245363reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/s12936-018-2414-2Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2453632026-05-22T06:33:51Z
dc.title.none.fl_str_mv Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
spellingShingle Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
Arévalo-Pinzón, Gabriela
Plasmodium vivax
Malaria
NAPPA array
IVTT protein expression
Protein–protein interaction
title_short Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_full Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_fullStr Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_full_unstemmed Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
title_sort Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites
dc.creator.none.fl_str_mv Arévalo-Pinzón, Gabriela
González-González, María
Suárez, Carlos Fernando
Curtidor, Hernando
Carabias-Sánchez, Javier
Muro, Antonio
LaBaer, Joshua
Patarroyo, M. A.
Fuentes, Manuel
author Arévalo-Pinzón, Gabriela
author_facet Arévalo-Pinzón, Gabriela
González-González, María
Suárez, Carlos Fernando
Curtidor, Hernando
Carabias-Sánchez, Javier
Muro, Antonio
LaBaer, Joshua
Patarroyo, M. A.
Fuentes, Manuel
author_role author
author2 González-González, María
Suárez, Carlos Fernando
Curtidor, Hernando
Carabias-Sánchez, Javier
Muro, Antonio
LaBaer, Joshua
Patarroyo, M. A.
Fuentes, Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Colciencias (Colombia)
Instituto de Salud Carlos III
Junta de Castilla y León
Fundación Memoria de D. Samuel Solorzano Barruso
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Plasmodium vivax
Malaria
NAPPA array
IVTT protein expression
Protein–protein interaction
topic Plasmodium vivax
Malaria
NAPPA array
IVTT protein expression
Protein–protein interaction
description © The Author(s).
publishDate 2018
dc.date.none.fl_str_mv 2018
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/245363
url http://hdl.handle.net/10261/245363
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/s12936-018-2414-2
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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