Use of adenoviral vectors with a minimal cytomegalovirus promoter
E1-deleted recombinant adenoviruses 5 are widely used as gene vectors for basic and clinical research. Most adenoviral constructions bear an expression cassette inserted into the E1 region of their genome close to the 5′ end and include a heterologous promoter. The major immediate early human cytome...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2018 |
| País: | España |
| Recursos: | Universidad de Barcelona |
| Repositório: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/223531 |
| Acesso em linha: | https://hdl.handle.net/2445/223531 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Animals Cèl·lules Citomegalovirus Adenovirus Cells Cytomegaloviruses Adenoviruses |
| Resumo: | E1-deleted recombinant adenoviruses 5 are widely used as gene vectors for basic and clinical research. Most adenoviral constructions bear an expression cassette inserted into the E1 region of their genome close to the 5′ end and include a heterologous promoter. The major immediate early human cytomegalovirus (hCMV) and the Rous sarcoma virus promoters, which have fairly high and consistent activity, are among the most extensively used. Nevertheless, expression of delivered protein from these promoters is often excessive, and weaker transcriptional activity would be desirable. |
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