Humoral immune response to different routes of myxomatosis vaccine application

[EN] The aim of our study was to monitor the dynamics of the serological response to different application routes of live attenuated myxomatosis vaccine. The study included 42 Californian breed rabbits, aged 3 mo, of both sexes. They were separated into 7 groups: 6 experimental and 1 control. All ex...

Descripción completa

Detalles Bibliográficos
Autores: Manev, I., Genova, K., Lavazza, A., Capucci, L.
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/105082
Acceso en línea:https://riunet.upv.es/handle/10251/105082
Access Level:acceso abierto
Palabra clave:Myxoma virus
Myxomatosis vaccination
ELISA
Antibody response
id ES_283faf30bee468a41e6aaff36667bd36
oai_identifier_str oai:riunet.upv.es:10251/105082
network_acronym_str ES
network_name_str España
repository_id_str
spelling Humoral immune response to different routes of myxomatosis vaccine applicationManev, I.Genova, K.Lavazza, A.Capucci, L.Myxoma virusMyxomatosis vaccinationELISAAntibody response[EN] The aim of our study was to monitor the dynamics of the serological response to different application routes of live attenuated myxomatosis vaccine. The study included 42 Californian breed rabbits, aged 3 mo, of both sexes. They were separated into 7 groups: 6 experimental and 1 control. All experimental groups were vaccinated on day 0 with a single dose of myxomatosis vaccine (min 103.3 tissue culture infective dose 50 [TCID50], max 105.8 TCID50). Three of the groups were injected with monovalent attenuated myxomatosis vaccine using different types of application: intradermal (i.d.), intramuscular (i.m.) and subcutaneous (s.c.). The other 3 groups were injected with bivalent attenuated vaccine against myxomatosis and rabbit haemorrhagic disease; again the routes of administration were i.d., i.m. and s.c.. There were no clinical signs or serious side effects after vaccination. The serological response was evaluated on days 7, 15 and 30 with a monoclonal antibody based-competition enzyme-linked immunosorbent assay (cELISA). More rapid and potent humoral response was detected in groups with i.d. inoculation in comparison to i.m. and s.c. routes. Vaccination with monovalent vaccine against myxomatosis induced higher antibody titre in comparison to bivalent vaccine. Our study showed that the vaccine application route and the type of vaccine used influence the speed and intensity of antibody response.Universitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-06-28journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/105082reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1050822026-06-13T07:49:27Z
dc.title.none.fl_str_mv Humoral immune response to different routes of myxomatosis vaccine application
title Humoral immune response to different routes of myxomatosis vaccine application
spellingShingle Humoral immune response to different routes of myxomatosis vaccine application
Manev, I.
Myxoma virus
Myxomatosis vaccination
ELISA
Antibody response
title_short Humoral immune response to different routes of myxomatosis vaccine application
title_full Humoral immune response to different routes of myxomatosis vaccine application
title_fullStr Humoral immune response to different routes of myxomatosis vaccine application
title_full_unstemmed Humoral immune response to different routes of myxomatosis vaccine application
title_sort Humoral immune response to different routes of myxomatosis vaccine application
dc.creator.none.fl_str_mv Manev, I.
Genova, K.
Lavazza, A.
Capucci, L.
author Manev, I.
author_facet Manev, I.
Genova, K.
Lavazza, A.
Capucci, L.
author_role author
author2 Genova, K.
Lavazza, A.
Capucci, L.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Myxoma virus
Myxomatosis vaccination
ELISA
Antibody response
topic Myxoma virus
Myxomatosis vaccination
ELISA
Antibody response
description [EN] The aim of our study was to monitor the dynamics of the serological response to different application routes of live attenuated myxomatosis vaccine. The study included 42 Californian breed rabbits, aged 3 mo, of both sexes. They were separated into 7 groups: 6 experimental and 1 control. All experimental groups were vaccinated on day 0 with a single dose of myxomatosis vaccine (min 103.3 tissue culture infective dose 50 [TCID50], max 105.8 TCID50). Three of the groups were injected with monovalent attenuated myxomatosis vaccine using different types of application: intradermal (i.d.), intramuscular (i.m.) and subcutaneous (s.c.). The other 3 groups were injected with bivalent attenuated vaccine against myxomatosis and rabbit haemorrhagic disease; again the routes of administration were i.d., i.m. and s.c.. There were no clinical signs or serious side effects after vaccination. The serological response was evaluated on days 7, 15 and 30 with a monoclonal antibody based-competition enzyme-linked immunosorbent assay (cELISA). More rapid and potent humoral response was detected in groups with i.d. inoculation in comparison to i.m. and s.c. routes. Vaccination with monovalent vaccine against myxomatosis induced higher antibody titre in comparison to bivalent vaccine. Our study showed that the vaccine application route and the type of vaccine used influence the speed and intensity of antibody response.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-06-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/105082
url https://riunet.upv.es/handle/10251/105082
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de València
publisher.none.fl_str_mv Universitat Politècnica de València
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404934335627264
score 15,301629

Similares en LA Referencia