Microbiomes associated with infective stages of root-knot and lesion nematodes in soil

Endoparasitic root-knot (Meloidogyne spp.) and lesion (Pratylenchus spp.) nematodes cause considerable damage in agriculture. Before they invade roots to complete their life cycle, soil microbes can attach to their cuticle or surface coat and antagonize the nematode directly or by induction of host...

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Autores: Elhady, A., Giné Blasco, Ariadna|||0000-0002-3598-4818, Topalovic, Olivera, Sorensen, E.R., Sorribas Royo, Francisco Javier|||0000-0001-7465-7353, Heuer, Holger
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/347856
Acesso em linha:https://hdl.handle.net/2117/347856
https://dx.doi.org/10.1371/journal.pone.0177145
Access Level:acceso abierto
Palavra-chave:Root-knot nematodes
Microbiomes
Agrotech
Nematodes -- Control
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
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spelling Microbiomes associated with infective stages of root-knot and lesion nematodes in soilElhady, A.Giné Blasco, Ariadna|||0000-0002-3598-4818Topalovic, OliveraSorensen, E.R.Sorribas Royo, Francisco Javier|||0000-0001-7465-7353Heuer, HolgerRoot-knot nematodesMicrobiomesAgrotechNematodes -- ControlÀrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::FitopatologiaEndoparasitic root-knot (Meloidogyne spp.) and lesion (Pratylenchus spp.) nematodes cause considerable damage in agriculture. Before they invade roots to complete their life cycle, soil microbes can attach to their cuticle or surface coat and antagonize the nematode directly or by induction of host plant defenses. We investigated whether the nematode-Associated microbiome in soil differs between infective stages of Meloidogyne incognita and Pratylenchus penetrans, and whether it is affected by variation in the composition of microbial communities among soils. Nematodes were incubated in suspensions of five organically and two integrated horticultural production soils, recovered by sieving and analyzed for attached bacteria and fungi after washing off loosely adhering microbes. Significant effects of the soil type and nematode species on nematode-Associated fungi and bacteria were revealed as analyzed by community profiling using denaturing gradient gel electrophoresis. Attached microbes represented a small specific subset of the soil microbiome. Two organic soils had very similar bacterial and fungal community profiles, but one of them was strongly suppressive towards root-knot nematodes. They were selected for deep amplicon sequencing of bacterial 16S rRNA genes and fungal ITS. Significant differences among the microbiomes associated with the two species in both soils suggested specific surface epitopes. Among the 28 detected bacterial classes, Betaproteobacteria, Bacilli and Actinobacteria were the most abundant. The most frequently detected fungal genera were Malassezia, Aspergillus and Cladosporium. Attached microbiomes did not statistically differ between these two soils. However, Malassezia globosa and four fungal species of the family Plectosphaerellaceae, and the bacterium Neorhizobium galegae were strongly enriched on M. incognita in the suppressive soil. In conclusion, the highly specific attachment of microbes to infective stages of phytonematodes in soil suggested an ecological role of this association and might be involved in soil suppressiveness towards them.Public Library of Science (PLOS)20172017-05-0120212021-06-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/347856https://dx.doi.org/10.1371/journal.pone.0177145reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3478562026-05-27T15:37:01Z
dc.title.none.fl_str_mv Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
title Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
spellingShingle Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
Elhady, A.
Root-knot nematodes
Microbiomes
Agrotech
Nematodes -- Control
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
title_short Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
title_full Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
title_fullStr Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
title_full_unstemmed Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
title_sort Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
dc.creator.none.fl_str_mv Elhady, A.
Giné Blasco, Ariadna|||0000-0002-3598-4818
Topalovic, Olivera
Sorensen, E.R.
Sorribas Royo, Francisco Javier|||0000-0001-7465-7353
Heuer, Holger
author Elhady, A.
author_facet Elhady, A.
Giné Blasco, Ariadna|||0000-0002-3598-4818
Topalovic, Olivera
Sorensen, E.R.
Sorribas Royo, Francisco Javier|||0000-0001-7465-7353
Heuer, Holger
author_role author
author2 Giné Blasco, Ariadna|||0000-0002-3598-4818
Topalovic, Olivera
Sorensen, E.R.
Sorribas Royo, Francisco Javier|||0000-0001-7465-7353
Heuer, Holger
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Root-knot nematodes
Microbiomes
Agrotech
Nematodes -- Control
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
topic Root-knot nematodes
Microbiomes
Agrotech
Nematodes -- Control
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
description Endoparasitic root-knot (Meloidogyne spp.) and lesion (Pratylenchus spp.) nematodes cause considerable damage in agriculture. Before they invade roots to complete their life cycle, soil microbes can attach to their cuticle or surface coat and antagonize the nematode directly or by induction of host plant defenses. We investigated whether the nematode-Associated microbiome in soil differs between infective stages of Meloidogyne incognita and Pratylenchus penetrans, and whether it is affected by variation in the composition of microbial communities among soils. Nematodes were incubated in suspensions of five organically and two integrated horticultural production soils, recovered by sieving and analyzed for attached bacteria and fungi after washing off loosely adhering microbes. Significant effects of the soil type and nematode species on nematode-Associated fungi and bacteria were revealed as analyzed by community profiling using denaturing gradient gel electrophoresis. Attached microbes represented a small specific subset of the soil microbiome. Two organic soils had very similar bacterial and fungal community profiles, but one of them was strongly suppressive towards root-knot nematodes. They were selected for deep amplicon sequencing of bacterial 16S rRNA genes and fungal ITS. Significant differences among the microbiomes associated with the two species in both soils suggested specific surface epitopes. Among the 28 detected bacterial classes, Betaproteobacteria, Bacilli and Actinobacteria were the most abundant. The most frequently detected fungal genera were Malassezia, Aspergillus and Cladosporium. Attached microbiomes did not statistically differ between these two soils. However, Malassezia globosa and four fungal species of the family Plectosphaerellaceae, and the bacterium Neorhizobium galegae were strongly enriched on M. incognita in the suppressive soil. In conclusion, the highly specific attachment of microbes to infective stages of phytonematodes in soil suggested an ecological role of this association and might be involved in soil suppressiveness towards them.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-05-01
2021
2021-06-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/347856
https://dx.doi.org/10.1371/journal.pone.0177145
url https://hdl.handle.net/2117/347856
https://dx.doi.org/10.1371/journal.pone.0177145
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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