Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2

Hypothesis: Dental bleaching with H2O2 is a common daily practice in dentistry to correct discoloration of anterior teeth. The aim of this study has been to determine whether this treatment of human teeth affects growth, differentiation and activity of osteoclast-like cells, as well as the putative...

Descripción completa

Detalles Bibliográficos
Autores: Torres-Rodriguez, Carolina, Portolés Pérez, María Teresa, Matesanz Sancho, María Concepción, Linares, Javier, Feito Castellano, María José, Izquierdo Barba, Isabel, Esbrit, Pedro, Vallet Regí, María Dulce Nombre
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/23097
Acceso en línea:https://hdl.handle.net/20.500.14352/23097
Access Level:acceso abierto
Palabra clave:546
615.46
Dental bleaching
External cervical resorption
Osteoclast
Fibroblast growth factor 2
Osteostatin
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
id ES_2807695f19dbb8925b3c19ee3076cc6d
oai_identifier_str oai:docta.ucm.es:20.500.14352/23097
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2Torres-Rodriguez, CarolinaPortolés Pérez, María TeresaMatesanz Sancho, María ConcepciónLinares, JavierFeito Castellano, María JoséIzquierdo Barba, IsabelEsbrit, PedroVallet Regí, María Dulce Nombre546615.46Dental bleachingExternal cervical resorptionOsteoclastFibroblast growth factor 2OsteostatinMaterialesQuímica inorgánica (Química)3312 Tecnología de Materiales2303 Química InorgánicaHypothesis: Dental bleaching with H2O2 is a common daily practice in dentistry to correct discoloration of anterior teeth. The aim of this study has been to determine whether this treatment of human teeth affects growth, differentiation and activity of osteoclast-like cells, as well as the putative modulatory action of osteostatin and fibroblast growth factor 2 (FGF-2). Experiments: Previously to the in vitro assays, structural, physical-chemical and morphological features of teeth after bleaching were studied. Osteoclast-like cells were cultured on human dentin disks, pre-treated or not with 38% H2O2 bleaching gel, in the presence or absence of osteostatin (100 nM) or FGF-2 (1 ng/ml). Cell proliferation and viability, intracellular content of reactive oxygen species (ROS), pro-inflammatory cytokine (IL-6 and TNF alpha) secretion and resorption activity were evaluated. Findings: Bleaching treatment failed to affect either the structural or the chemical features of both enamel and dentin, except for slight morphological changes, increased porosity in the most superficial parts (enamel), and a moderate increase in the wettability degree. In this scenario, bleaching produced an increased osteoclast-like cell proliferation but decreased cell viability and cytokine secretion, while it augmented resorption activity on dentin. The presence of either osteostatin or FGF-2 reduced the osteoclast-like cell proliferation induced by bleaching. FGF-2 enhanced ROS content, whereas osteostatin decreased ROS but increased TNF alpha secretion. The bleaching effect on resorption activity was increased by osteostatin, but this effect was less evident with FGF-2. Conclusions: These findings further confirm the deleterious effects of tooth bleaching by affecting osteoclast growth and function as well as different modulatory actions of osteostatin and FGF-2. (C) 2015 Elsevier Inc. All rights reserved.ElsevierUniversidad Complutense de Madrid20162016-01-0120162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/23097reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/230972026-06-02T12:44:21Z
dc.title.none.fl_str_mv Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
title Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
spellingShingle Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
Torres-Rodriguez, Carolina
546
615.46
Dental bleaching
External cervical resorption
Osteoclast
Fibroblast growth factor 2
Osteostatin
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
title_short Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
title_full Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
title_fullStr Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
title_full_unstemmed Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
title_sort Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
dc.creator.none.fl_str_mv Torres-Rodriguez, Carolina
Portolés Pérez, María Teresa
Matesanz Sancho, María Concepción
Linares, Javier
Feito Castellano, María José
Izquierdo Barba, Isabel
Esbrit, Pedro
Vallet Regí, María Dulce Nombre
author Torres-Rodriguez, Carolina
author_facet Torres-Rodriguez, Carolina
Portolés Pérez, María Teresa
Matesanz Sancho, María Concepción
Linares, Javier
Feito Castellano, María José
Izquierdo Barba, Isabel
Esbrit, Pedro
Vallet Regí, María Dulce Nombre
author_role author
author2 Portolés Pérez, María Teresa
Matesanz Sancho, María Concepción
Linares, Javier
Feito Castellano, María José
Izquierdo Barba, Isabel
Esbrit, Pedro
Vallet Regí, María Dulce Nombre
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 546
615.46
Dental bleaching
External cervical resorption
Osteoclast
Fibroblast growth factor 2
Osteostatin
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
topic 546
615.46
Dental bleaching
External cervical resorption
Osteoclast
Fibroblast growth factor 2
Osteostatin
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
description Hypothesis: Dental bleaching with H2O2 is a common daily practice in dentistry to correct discoloration of anterior teeth. The aim of this study has been to determine whether this treatment of human teeth affects growth, differentiation and activity of osteoclast-like cells, as well as the putative modulatory action of osteostatin and fibroblast growth factor 2 (FGF-2). Experiments: Previously to the in vitro assays, structural, physical-chemical and morphological features of teeth after bleaching were studied. Osteoclast-like cells were cultured on human dentin disks, pre-treated or not with 38% H2O2 bleaching gel, in the presence or absence of osteostatin (100 nM) or FGF-2 (1 ng/ml). Cell proliferation and viability, intracellular content of reactive oxygen species (ROS), pro-inflammatory cytokine (IL-6 and TNF alpha) secretion and resorption activity were evaluated. Findings: Bleaching treatment failed to affect either the structural or the chemical features of both enamel and dentin, except for slight morphological changes, increased porosity in the most superficial parts (enamel), and a moderate increase in the wettability degree. In this scenario, bleaching produced an increased osteoclast-like cell proliferation but decreased cell viability and cytokine secretion, while it augmented resorption activity on dentin. The presence of either osteostatin or FGF-2 reduced the osteoclast-like cell proliferation induced by bleaching. FGF-2 enhanced ROS content, whereas osteostatin decreased ROS but increased TNF alpha secretion. The bleaching effect on resorption activity was increased by osteostatin, but this effect was less evident with FGF-2. Conclusions: These findings further confirm the deleterious effects of tooth bleaching by affecting osteoclast growth and function as well as different modulatory actions of osteostatin and FGF-2. (C) 2015 Elsevier Inc. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/23097
url https://hdl.handle.net/20.500.14352/23097
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404921631080448
score 15.300719

Similares en LA Referencia