Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism

10 p., 8 figures and bibliography

Detalhes bibliográficos
Autores: Sánchez Martínez, Elisa, Rubio, V. C., Cerdá-Reverter, José Miguel
Formato: artículo
Fecha de publicación:2009
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/47803
Acesso em linha:http://hdl.handle.net/10261/47803
Access Level:acceso abierto
Palavra-chave:Melanocyte-stimulating hormone (MSH)
Proopiomelanocortin
MC5R
Phosphodiesterase inhibitor
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spelling Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolismSánchez Martínez, ElisaRubio, V. C.Cerdá-Reverter, José MiguelMelanocyte-stimulating hormone (MSH)ProopiomelanocortinMC5RPhosphodiesterase inhibitor10 p., 8 figures and bibliographyThe melanocortin 5 receptor (MC5R) plays a key role in the regulation of exocrine secretion in mammalian species. This receptor has also been characterized in some fish species but its function is unknown. We report the molecular and pharmacological characterization, as well as the tissue expression pattern, of sea bass MC5R. Cloning of five active alleles showing different levels of sensitivity to endogenous melanocortin and one non-functional allele demonstrate the allelic complexity of the MC5R locus. The sea bass receptor was activated by all the melanocortins tested, with ACTH and desacetyl-MSH and -MSH showing the lowest efficiency. The acetylation of the MSH isoforms seems to be critical for the effectiveness of the agonist. Agouti-related protein had no effect on basal or agonist-stimulated activation of the receptor. SbMC5R was mainly expressed in the brain but lower expression levels were found in several peripheral tissues, including liver. Progressive fasting did not induce up- or downregulation of hypothalamic MC5R expression, suggesting that central MC5R is not involved in the regulation of food intake in the sea bass. MTII, a sbMC5R agonist, stimulated hepatic lipolysis in vitro, measured as free fatty acid release into the culture medium after melanocortin agonist exposure of liver fragments, suggesting that MC5R is involved in the regulation of hepatic lipid metabolism. Taken together, the data suggest that different allelic combinations may confer differential sensitivity to endogenous melanocortin in tissues where MC5R is expressed and, by extension, in hepatic lipid metabolism.Funded by AGL2004-08137-C04-04; AGL2007-65744-C03-02; CSD 2007-00002 from the Spanish Science and Innovation Ministry to J. M. C.-R. V. C. R. was recipient of a "Juan de la Cierva" research contract (2007) from the Spanish Science and Innovation Ministry.Peer reviewedCompany of Biologists201220122009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/47803reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/jeb.035121info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/478032026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
title Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
spellingShingle Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
Sánchez Martínez, Elisa
Melanocyte-stimulating hormone (MSH)
Proopiomelanocortin
MC5R
Phosphodiesterase inhibitor
title_short Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
title_full Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
title_fullStr Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
title_full_unstemmed Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
title_sort Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism
dc.creator.none.fl_str_mv Sánchez Martínez, Elisa
Rubio, V. C.
Cerdá-Reverter, José Miguel
author Sánchez Martínez, Elisa
author_facet Sánchez Martínez, Elisa
Rubio, V. C.
Cerdá-Reverter, José Miguel
author_role author
author2 Rubio, V. C.
Cerdá-Reverter, José Miguel
author2_role author
author
dc.subject.none.fl_str_mv Melanocyte-stimulating hormone (MSH)
Proopiomelanocortin
MC5R
Phosphodiesterase inhibitor
topic Melanocyte-stimulating hormone (MSH)
Proopiomelanocortin
MC5R
Phosphodiesterase inhibitor
description 10 p., 8 figures and bibliography
publishDate 2009
dc.date.none.fl_str_mv 2009
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/47803
url http://hdl.handle.net/10261/47803
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/jeb.035121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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