Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum

Elucidation of the cell cycle of dinoflagellates is essential to understand the processes leading to their massive proliferations, known as harmful algal blooms. In this study, we used imaging flow cytometry (IFC) to monitor the changes in DNA content and nuclear and cell morphology that occur durin...

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Detalles Bibliográficos
Autores: Dapena, Carlos, Bravo, Isabel, Cuadrado, Ángeles, Figueroa, Rosa Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/321605
Acceso en línea:http://hdl.handle.net/10261/321605
Access Level:acceso abierto
Palabra clave:Cell-cycle
Medio Marino
Centro Oceanográfico de Vigo
Cytoplasmic-to-nuclear ratio (CNR)
Dinoflagellates
Growth
Nuclear size
ecology
growth
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repository_id_str
spelling Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutumDapena, CarlosBravo, IsabelCuadrado, ÁngelesFigueroa, Rosa IsabelCell-cycleMedio MarinoCentro Oceanográfico de VigoCytoplasmic-to-nuclear ratio (CNR)DinoflagellatesGrowthNuclear sizeecologygrowthElucidation of the cell cycle of dinoflagellates is essential to understand the processes leading to their massive proliferations, known as harmful algal blooms. In this study, we used imaging flow cytometry (IFC) to monitor the changes in DNA content and nuclear and cell morphology that occur during clonal growth of the toxic species Alexandrium minutum Halim. Our results indicate that the population was in S phase (C→2C DNA content) during the light period, whereas haploid cells with a C DNA content peaked only during a short interval of the dark period. The timing of the phases, identified based on the nuclear morphology and cytoplasmic-to-nuclear (CNR) ratio of the cells, suggests that the length of the G2/M phase is regulated by nutrient levels whereas the beginning of S phase is clock controlled. In addition we found that up to 7% of individual cells achieved a DNA content higher than 2C, indicative of either zygote formation and replication (homothallism), or of double-haploid cells able to divide (polyploid forms). Cells belonging to different cell cycle phases (G1-S-G2/M) could be readily discriminated based on nuclear size. Our study provides evidence of cell-cycle plasticity during clonal growth and unambiguously characterizes the cell-cycle phases of this dinoflagellate species.SI202320232015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/321605reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Vigohttp://www.sciencedirect.com/science/article/pii/S1434461015000024info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3216052026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
title Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
spellingShingle Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
Dapena, Carlos
Cell-cycle
Medio Marino
Centro Oceanográfico de Vigo
Cytoplasmic-to-nuclear ratio (CNR)
Dinoflagellates
Growth
Nuclear size
ecology
growth
title_short Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
title_full Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
title_fullStr Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
title_full_unstemmed Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
title_sort Nuclear and cell morphological changes during the cell cycle and growth of the toxic dinoflagellate Alexandrium minutum
dc.creator.none.fl_str_mv Dapena, Carlos
Bravo, Isabel
Cuadrado, Ángeles
Figueroa, Rosa Isabel
author Dapena, Carlos
author_facet Dapena, Carlos
Bravo, Isabel
Cuadrado, Ángeles
Figueroa, Rosa Isabel
author_role author
author2 Bravo, Isabel
Cuadrado, Ángeles
Figueroa, Rosa Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv Cell-cycle
Medio Marino
Centro Oceanográfico de Vigo
Cytoplasmic-to-nuclear ratio (CNR)
Dinoflagellates
Growth
Nuclear size
ecology
growth
topic Cell-cycle
Medio Marino
Centro Oceanográfico de Vigo
Cytoplasmic-to-nuclear ratio (CNR)
Dinoflagellates
Growth
Nuclear size
ecology
growth
description Elucidation of the cell cycle of dinoflagellates is essential to understand the processes leading to their massive proliferations, known as harmful algal blooms. In this study, we used imaging flow cytometry (IFC) to monitor the changes in DNA content and nuclear and cell morphology that occur during clonal growth of the toxic species Alexandrium minutum Halim. Our results indicate that the population was in S phase (C→2C DNA content) during the light period, whereas haploid cells with a C DNA content peaked only during a short interval of the dark period. The timing of the phases, identified based on the nuclear morphology and cytoplasmic-to-nuclear (CNR) ratio of the cells, suggests that the length of the G2/M phase is regulated by nutrient levels whereas the beginning of S phase is clock controlled. In addition we found that up to 7% of individual cells achieved a DNA content higher than 2C, indicative of either zygote formation and replication (homothallism), or of double-haploid cells able to divide (polyploid forms). Cells belonging to different cell cycle phases (G1-S-G2/M) could be readily discriminated based on nuclear size. Our study provides evidence of cell-cycle plasticity during clonal growth and unambiguously characterizes the cell-cycle phases of this dinoflagellate species.
publishDate 2015
dc.date.none.fl_str_mv 2015
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/321605
url http://hdl.handle.net/10261/321605
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Vigo
http://www.sciencedirect.com/science/article/pii/S1434461015000024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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