Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1

Background: The present work aims to investigate the antioxidant and antimicrobial activities as well as the potential of DCS1 lipopeptides produced by Bacillus methylotrophicus DCS1 strain at inhibition and disruption of biofilm formation. Results: The produced biosurfactants were characterized as...

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Detalles Bibliográficos
Autores: Jemil, Nawel, Manresa Presas, Ma. Ángeles (María Ángeles), Nasri, Moncef, Hmide, Noomen, Ben Ayed, Hanen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/121633
Acceso en línea:https://hdl.handle.net/2445/121633
Access Level:acceso abierto
Palabra clave:Antioxidants
Bacils
Bacillus (Bacteria)
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spelling Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1Jemil, NawelManresa Presas, Ma. Ángeles (María Ángeles)Nasri, MoncefHmide, NoomenBen Ayed, HanenAntioxidantsBacilsAntioxidantsBacillus (Bacteria)Background: The present work aims to investigate the antioxidant and antimicrobial activities as well as the potential of DCS1 lipopeptides produced by Bacillus methylotrophicus DCS1 strain at inhibition and disruption of biofilm formation. Results: The produced biosurfactants were characterized as lipopeptides molecules by using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR). The DCS1 lipopeptides were assayed for their antioxidant activity through five different tests. The scavenging effect on DPPH radicals at a concentration of 1 mg mL−1 was 80.6%. The reducing power reached a maximum value of 3.0 (OD700 nm) at 2 mg mL−1 . Moreover, the DCS1 lipopeptides exhibited a strong inhibition of β-carotene bleaching by linoleic acid assay with 80.8% at 1 mg mL−1 and showed good chelating ability and lipid peroxidation inhibition. The in vitro antimicrobial activity of DCS1 lipopeptides showed that they display significant antibacterial and antifungal activities. The anti-adhesive activity of DCS1 lipopeptides was evaluated against several pathogenic microorganisms. The lipopeptides showed excellent anti-adhesive activity, even at low concentrations, in a polystyrene surface pre-treatment against all the microorganisms tested. Further, they can disrupt performed biofilms. Conclusion: This study shows the potentiality of DCS1 lipopeptides as natural antioxidants, antimicrobial and/or anti-adhesive agent for several biomedical and industrial applications.BioMed Central2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/121633Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1186/s12866-017-1050-2BMC Microbiology, 2017, vol. 17, num. 144https://doi.org/10.1186/s12866-017-1050-2cc-by (c) Jemil, Nawel et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1216332026-05-27T06:46:51Z
dc.title.none.fl_str_mv Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
title Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
spellingShingle Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
Jemil, Nawel
Antioxidants
Bacils
Antioxidants
Bacillus (Bacteria)
title_short Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
title_full Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
title_fullStr Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
title_full_unstemmed Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
title_sort Antioxidant properties, antimicrobial and anti-adhesive acivities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
dc.creator.none.fl_str_mv Jemil, Nawel
Manresa Presas, Ma. Ángeles (María Ángeles)
Nasri, Moncef
Hmide, Noomen
Ben Ayed, Hanen
author Jemil, Nawel
author_facet Jemil, Nawel
Manresa Presas, Ma. Ángeles (María Ángeles)
Nasri, Moncef
Hmide, Noomen
Ben Ayed, Hanen
author_role author
author2 Manresa Presas, Ma. Ángeles (María Ángeles)
Nasri, Moncef
Hmide, Noomen
Ben Ayed, Hanen
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antioxidants
Bacils
Antioxidants
Bacillus (Bacteria)
topic Antioxidants
Bacils
Antioxidants
Bacillus (Bacteria)
description Background: The present work aims to investigate the antioxidant and antimicrobial activities as well as the potential of DCS1 lipopeptides produced by Bacillus methylotrophicus DCS1 strain at inhibition and disruption of biofilm formation. Results: The produced biosurfactants were characterized as lipopeptides molecules by using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR). The DCS1 lipopeptides were assayed for their antioxidant activity through five different tests. The scavenging effect on DPPH radicals at a concentration of 1 mg mL−1 was 80.6%. The reducing power reached a maximum value of 3.0 (OD700 nm) at 2 mg mL−1 . Moreover, the DCS1 lipopeptides exhibited a strong inhibition of β-carotene bleaching by linoleic acid assay with 80.8% at 1 mg mL−1 and showed good chelating ability and lipid peroxidation inhibition. The in vitro antimicrobial activity of DCS1 lipopeptides showed that they display significant antibacterial and antifungal activities. The anti-adhesive activity of DCS1 lipopeptides was evaluated against several pathogenic microorganisms. The lipopeptides showed excellent anti-adhesive activity, even at low concentrations, in a polystyrene surface pre-treatment against all the microorganisms tested. Further, they can disrupt performed biofilms. Conclusion: This study shows the potentiality of DCS1 lipopeptides as natural antioxidants, antimicrobial and/or anti-adhesive agent for several biomedical and industrial applications.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/121633
url https://hdl.handle.net/2445/121633
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/s12866-017-1050-2
BMC Microbiology, 2017, vol. 17, num. 144
https://doi.org/10.1186/s12866-017-1050-2
dc.rights.none.fl_str_mv cc-by (c) Jemil, Nawel et al., 2017
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Jemil, Nawel et al., 2017
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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