The Use of Genomic Screening for the Detection of Chromosomal Abnormalities in the Domestic Horse: Five New Cases of 65,XXY Syndrome in the Pura Raza Español Breed

Sex chromosomal abnormalities are a major cause of reproductive failure in horses. While some horses with abnormal karyotypes exhibit reproductive abnormalities, others appear normal, remaining misdiagnosed until late age. The Pura Raza Español breeding program screens all horses for these abnormali...

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Detalles Bibliográficos
Autores: Valera Córdoba, María Mercedes, Karlau, Ayelén, Anaya, Gabriel, Bugno-Poniewierska, Monika, Molina, Antonio, Encina, Ana, Azor, Pedro, Demyda-Peyrás, Sebastián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:dnet:idus________::d92ec2b834c7ec90b83e466aeeeb0dee
Acceso en línea:https://hdl.handle.net/11441/186125
https://doi.org/10.3390/ani14172560
Access Level:acceso abierto
Palabra clave:CNA
cytogenetics
Equine
Genomic chromosomal screening
Sex chromosomal abnormalities
65XXY
Descripción
Sumario:Sex chromosomal abnormalities are a major cause of reproductive failure in horses. While some horses with abnormal karyotypes exhibit reproductive abnormalities, others appear normal, remaining misdiagnosed until late age. The Pura Raza Español breeding program screens all horses for these abnormalities using genomics before adding them to the studbook. This process includes an initial assessment using the results of Short Tandem Repeat (STR) parentage testing, followed by confirmation using Single-Nucleotide Polymorphism (SNP) analysis for copy number aberrations. Hereby, we identified five new cases of 65,XXY syndrome among 27,330 foals over two breeding seasons. These horses were flagged as possibly affected by a chromosomal number aberration (CNA) due to abnormal STR and confirmed as 65,XXY by genomic analysis. All of them showed a male phenotype. One horse showed abnormal gonad development, whereas the others did not have visible abnormalities. This study represents the largest group of horses diagnosed with 65,XXY and emphasizes the importance of genomic screening for chromosomal testing.