Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity

Background: The vasoactive intestinal peptide (VIP) receptors VPAC1 and VPAC2 mediate anti-inflammatory and immunoregulatory responses in rheumatoid arthritis (RA). Data on the expression of these receptors could complement clinical assessment in the management of RA.Our goal was to investigate the...

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Autores: Valiño Seoane, Iria, Ortiz, Ana M., Piris, Lorena, Lamana Domínguez, Amalia, Juarranz Moratilla, Yasmina, García Vicuña, Rosario, González Álvaro, Isidoro, Pérez Gomariz, Rosa María, Martínez Mora, María Del Carmen
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/23121
Acceso en línea:https://hdl.handle.net/20.500.14352/23121
Access Level:acceso abierto
Palabra clave:577.112
616.72-002
VPAC1 Recepto
Arthritis
Immune receptors
Gene expression
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/23121
network_acronym_str ES
network_name_str España
repository_id_str
spelling Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease ActivityValiño Seoane, IriaOrtiz, Ana M.Piris, LorenaLamana Domínguez, AmaliaJuarranz Moratilla, YasminaGarcía Vicuña, RosarioGonzález Álvaro, IsidoroPérez Gomariz, Rosa MaríaMartínez Mora, María Del Carmen577.112616.72-002VPAC1 ReceptoArthritisImmune receptorsGene expressionReumatologíaBioquímica (Biología)3205.09 Reumatología2302 BioquímicaBackground: The vasoactive intestinal peptide (VIP) receptors VPAC1 and VPAC2 mediate anti-inflammatory and immunoregulatory responses in rheumatoid arthritis (RA). Data on the expression of these receptors could complement clinical assessment in the management of RA.Our goal was to investigate the correlation between expression of both receptors and the 28-Joint Disease Activity Score (DAS28) in peripheral blood mononuclear cells (PBMCs)from patients with early arthritis (EA). We also measured expression of IL-6 to evaluate theassociation between VIP receptors and systemic inflammation. Methods: We analyzed 250 blood samples collected at any of the 5 scheduled follow-up visits from 125 patients enrolled in the Princesa Early Arthritis Register Longitudinal study. Samples from 22 healthy donors were also analyzed. Sociodemographic, clinical, and therapeutic data were systematically recorded. mRNA expression levels were determined using realtime PCR. Then, longitudinal multivariate analyses were performed. Results: PBMCs from EA patients showed significantly higher expression of VPAC2 receptors at baseline compared to healthy donors (p<0.001). With time, however, VPAC2 expression tended to be significantly lower while VPAC1 receptor expression increased in correlation with a reduction in DAS28 index. Our results reveal that more severe inflammation, based on high levels of IL-6, is associated with lower expression of VPAC1 (p<0.001) and conversely with increased expression of VPAC2 (p<0.001). A major finding of this study is thatexpression of VPAC1 is lower in patients with increased disease activity (p = 0.001), thus making it possible to differentiate between patients with various degrees of clinical disease activity.Public Library of Sciences (PLOS)Universidad Complutense de Madrid20162016-02-0120162016-02-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/23121reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/231212026-06-02T12:44:21Z
dc.title.none.fl_str_mv Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
title Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
spellingShingle Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
Valiño Seoane, Iria
577.112
616.72-002
VPAC1 Recepto
Arthritis
Immune receptors
Gene expression
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
title_short Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
title_full Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
title_fullStr Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
title_full_unstemmed Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
title_sort Clinical Relevance of VPAC1 Receptor Expression in Early Arthritis: Association with IL-6 and Disease Activity
dc.creator.none.fl_str_mv Valiño Seoane, Iria
Ortiz, Ana M.
Piris, Lorena
Lamana Domínguez, Amalia
Juarranz Moratilla, Yasmina
García Vicuña, Rosario
González Álvaro, Isidoro
Pérez Gomariz, Rosa María
Martínez Mora, María Del Carmen
author Valiño Seoane, Iria
author_facet Valiño Seoane, Iria
Ortiz, Ana M.
Piris, Lorena
Lamana Domínguez, Amalia
Juarranz Moratilla, Yasmina
García Vicuña, Rosario
González Álvaro, Isidoro
Pérez Gomariz, Rosa María
Martínez Mora, María Del Carmen
author_role author
author2 Ortiz, Ana M.
Piris, Lorena
Lamana Domínguez, Amalia
Juarranz Moratilla, Yasmina
García Vicuña, Rosario
González Álvaro, Isidoro
Pérez Gomariz, Rosa María
Martínez Mora, María Del Carmen
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.112
616.72-002
VPAC1 Recepto
Arthritis
Immune receptors
Gene expression
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
topic 577.112
616.72-002
VPAC1 Recepto
Arthritis
Immune receptors
Gene expression
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
description Background: The vasoactive intestinal peptide (VIP) receptors VPAC1 and VPAC2 mediate anti-inflammatory and immunoregulatory responses in rheumatoid arthritis (RA). Data on the expression of these receptors could complement clinical assessment in the management of RA.Our goal was to investigate the correlation between expression of both receptors and the 28-Joint Disease Activity Score (DAS28) in peripheral blood mononuclear cells (PBMCs)from patients with early arthritis (EA). We also measured expression of IL-6 to evaluate theassociation between VIP receptors and systemic inflammation. Methods: We analyzed 250 blood samples collected at any of the 5 scheduled follow-up visits from 125 patients enrolled in the Princesa Early Arthritis Register Longitudinal study. Samples from 22 healthy donors were also analyzed. Sociodemographic, clinical, and therapeutic data were systematically recorded. mRNA expression levels were determined using realtime PCR. Then, longitudinal multivariate analyses were performed. Results: PBMCs from EA patients showed significantly higher expression of VPAC2 receptors at baseline compared to healthy donors (p<0.001). With time, however, VPAC2 expression tended to be significantly lower while VPAC1 receptor expression increased in correlation with a reduction in DAS28 index. Our results reveal that more severe inflammation, based on high levels of IL-6, is associated with lower expression of VPAC1 (p<0.001) and conversely with increased expression of VPAC2 (p<0.001). A major finding of this study is thatexpression of VPAC1 is lower in patients with increased disease activity (p = 0.001), thus making it possible to differentiate between patients with various degrees of clinical disease activity.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-02-01
2016
2016-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/23121
url https://hdl.handle.net/20.500.14352/23121
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Sciences (PLOS)
publisher.none.fl_str_mv Public Library of Sciences (PLOS)
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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