Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)

The apolipoprotein A-I (ApoA-I) is an essential component of the high density lipoproteins (HDL). In this study, the cDNA and genomic sequences of this apolipoprotein were characterized for first time in Solea senegalensis. The predicted polypeptide revealed conserved structural features including t...

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Autores: Román-Padilla, Javier, Rodríguez-Rúa, Ana, Manchado, Manuel, Hachero-Cruzado, Ismael
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313355
Acceso en línea:http://hdl.handle.net/10261/313355
Access Level:acceso abierto
Palabra clave:Acuicultura
Apolipoprotein A-I
Sede Central IEO
Gene expression
Synteny
Senegalese sole
Brain
HDL
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spelling Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)Román-Padilla, JavierRodríguez-Rúa, AnaManchado, ManuelHachero-Cruzado, IsmaelAcuiculturaApolipoprotein A-ISede Central IEOGene expressionSyntenySenegalese soleBrainHDLThe apolipoprotein A-I (ApoA-I) is an essential component of the high density lipoproteins (HDL). In this study, the cDNA and genomic sequences of this apolipoprotein were characterized for first time in Solea senegalensis. The predicted polypeptide revealed conserved structural features including ten repeats in the lipid-binding domain and some residues involved in cholesterol interaction and binding. The gene structure analysis identified four exons and three introns. Moreover, the synteny analysis revealed that apoA-I did not localize with other apolipoproteins indicating a divergent evolution with respect to the apoA-IV and apoE cluster. The phylogenetic analyses identified two distinct apoA-I paralogs in Ostariophysi (referred to as Ia and Ib) and only one (Ib) in Acanthopterygii. Whole-mount in situ hybridization located the apoA-I signal mainly in the yolk syncytial layer in lecitotrophic larval stages. Later at mouth opening, the mRNA signals were detected mainly in liver and intestine compatible with its role in the HDL formation. Moreover, a clear signal was detected in some regions of the brain, retina and neural cord suggesting a role in local regulation of cholesterol homeostasis. After metamorphosis, apoA-I was also detected in other tissues such as gills, head kidney and spleen suggesting a putative role in immunity. Expression analyses in larvae fed two diets with different triacylglycerol levels indicated that apoA-I mRNA levels were more associated to larval size and development than dietary lipid levels. Finally, qPCR analyses of immature and mature transcripts revealed distinct expression profiles suggesting a posttranscriptional regulatory mechanism.202320232016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/313355reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSede Central IEOinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3133552026-05-22T06:33:51Z
dc.title.none.fl_str_mv Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
title Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
spellingShingle Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
Román-Padilla, Javier
Acuicultura
Apolipoprotein A-I
Sede Central IEO
Gene expression
Synteny
Senegalese sole
Brain
HDL
title_short Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
title_full Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
title_fullStr Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
title_full_unstemmed Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
title_sort Molecular characterization and developmental expression patterns of apolipoprotein a-i in senegalese sole (solea senegalensis kaup)
dc.creator.none.fl_str_mv Román-Padilla, Javier
Rodríguez-Rúa, Ana
Manchado, Manuel
Hachero-Cruzado, Ismael
author Román-Padilla, Javier
author_facet Román-Padilla, Javier
Rodríguez-Rúa, Ana
Manchado, Manuel
Hachero-Cruzado, Ismael
author_role author
author2 Rodríguez-Rúa, Ana
Manchado, Manuel
Hachero-Cruzado, Ismael
author2_role author
author
author
dc.subject.none.fl_str_mv Acuicultura
Apolipoprotein A-I
Sede Central IEO
Gene expression
Synteny
Senegalese sole
Brain
HDL
topic Acuicultura
Apolipoprotein A-I
Sede Central IEO
Gene expression
Synteny
Senegalese sole
Brain
HDL
description The apolipoprotein A-I (ApoA-I) is an essential component of the high density lipoproteins (HDL). In this study, the cDNA and genomic sequences of this apolipoprotein were characterized for first time in Solea senegalensis. The predicted polypeptide revealed conserved structural features including ten repeats in the lipid-binding domain and some residues involved in cholesterol interaction and binding. The gene structure analysis identified four exons and three introns. Moreover, the synteny analysis revealed that apoA-I did not localize with other apolipoproteins indicating a divergent evolution with respect to the apoA-IV and apoE cluster. The phylogenetic analyses identified two distinct apoA-I paralogs in Ostariophysi (referred to as Ia and Ib) and only one (Ib) in Acanthopterygii. Whole-mount in situ hybridization located the apoA-I signal mainly in the yolk syncytial layer in lecitotrophic larval stages. Later at mouth opening, the mRNA signals were detected mainly in liver and intestine compatible with its role in the HDL formation. Moreover, a clear signal was detected in some regions of the brain, retina and neural cord suggesting a role in local regulation of cholesterol homeostasis. After metamorphosis, apoA-I was also detected in other tissues such as gills, head kidney and spleen suggesting a putative role in immunity. Expression analyses in larvae fed two diets with different triacylglycerol levels indicated that apoA-I mRNA levels were more associated to larval size and development than dietary lipid levels. Finally, qPCR analyses of immature and mature transcripts revealed distinct expression profiles suggesting a posttranscriptional regulatory mechanism.
publishDate 2016
dc.date.none.fl_str_mv 2016
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313355
url http://hdl.handle.net/10261/313355
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sede Central IEO
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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