Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry

The increasing frequency of episodes of harmful algal blooms of cyanobacterial origin is a risk for ecosystems and human health. The main human hazard may arise from drinking water supply and recreational water use. For this reason, efficient multiclass analytical methods are needed to assess the le...

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Detalles Bibliográficos
Autores: Filatova, Daria, Núñez Burcio, Oscar, Farré, Marinella
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/158200
Acceso en línea:https://hdl.handle.net/2445/158200
Access Level:acceso abierto
Palabra clave:Cromatografia de líquids
Cianobacteris
Toxines bacterianes
Liquid chromatography
Cyanobacteria
Bacterial toxins
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spelling Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometryFilatova, DariaNúñez Burcio, OscarFarré, MarinellaCromatografia de líquidsCianobacterisToxines bacterianesLiquid chromatographyCyanobacteriaBacterial toxinsThe increasing frequency of episodes of harmful algal blooms of cyanobacterial origin is a risk for ecosystems and human health. The main human hazard may arise from drinking water supply and recreational water use. For this reason, efficient multiclass analytical methods are needed to assess the level of cyanotoxins in water reservoirs and tackle these problems. This work describes the development of a fast, sensitive and robust analytical method for multiclass cyanotoxins determination based on dual solid-phase extraction (SPE) procedure using a polymeric cartridge first Oasis HLB (Waters Corporation, USA), and second, a graphitized non-porous carbon cartridge, SupelcleanTM ENVI-CarbTM (Sigma-Aldrich, USA), followed by ultra-high performance liquid chromatography high-resolution mass spectrometry (SPE-UHPLC-HRMS). This method enabled the analysis of cylindrospermopsin, anatoxin-a, nodularin and seven microcystins (MC-LR, MC-RR, MC-YR, MC-LA, MC-LY, MC-LW, MC-LF). The method limits of detection (MLOD) of the validated approach were between 4 and 150 pg/L. The analytical method was applied to assess the presence of the selected toxins in 21 samples collected in 3 natural water reservoirs in the Ter River in Catalonia (NE of Spain) used to produce drinking water for Barcelona city (Spain).MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/158200Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/toxins12040247Toxins, 2020, vol. 12, num. 4, p. 247https://doi.org/10.3390/toxins12040247info:eu-repo/grantAgreement/EC/H2020/722493cc-by (c) Filatova, Daria et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1582002026-05-27T06:46:51Z
dc.title.none.fl_str_mv Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
title Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
spellingShingle Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
Filatova, Daria
Cromatografia de líquids
Cianobacteris
Toxines bacterianes
Liquid chromatography
Cyanobacteria
Bacterial toxins
title_short Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
title_full Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
title_fullStr Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
title_full_unstemmed Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
title_sort Ultra-trace analysis of cyanotoxins by liquid chromatography coupled to high-resolution mass spectrometry
dc.creator.none.fl_str_mv Filatova, Daria
Núñez Burcio, Oscar
Farré, Marinella
author Filatova, Daria
author_facet Filatova, Daria
Núñez Burcio, Oscar
Farré, Marinella
author_role author
author2 Núñez Burcio, Oscar
Farré, Marinella
author2_role author
author
dc.subject.none.fl_str_mv Cromatografia de líquids
Cianobacteris
Toxines bacterianes
Liquid chromatography
Cyanobacteria
Bacterial toxins
topic Cromatografia de líquids
Cianobacteris
Toxines bacterianes
Liquid chromatography
Cyanobacteria
Bacterial toxins
description The increasing frequency of episodes of harmful algal blooms of cyanobacterial origin is a risk for ecosystems and human health. The main human hazard may arise from drinking water supply and recreational water use. For this reason, efficient multiclass analytical methods are needed to assess the level of cyanotoxins in water reservoirs and tackle these problems. This work describes the development of a fast, sensitive and robust analytical method for multiclass cyanotoxins determination based on dual solid-phase extraction (SPE) procedure using a polymeric cartridge first Oasis HLB (Waters Corporation, USA), and second, a graphitized non-porous carbon cartridge, SupelcleanTM ENVI-CarbTM (Sigma-Aldrich, USA), followed by ultra-high performance liquid chromatography high-resolution mass spectrometry (SPE-UHPLC-HRMS). This method enabled the analysis of cylindrospermopsin, anatoxin-a, nodularin and seven microcystins (MC-LR, MC-RR, MC-YR, MC-LA, MC-LY, MC-LW, MC-LF). The method limits of detection (MLOD) of the validated approach were between 4 and 150 pg/L. The analytical method was applied to assess the presence of the selected toxins in 21 samples collected in 3 natural water reservoirs in the Ter River in Catalonia (NE of Spain) used to produce drinking water for Barcelona city (Spain).
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/158200
url https://hdl.handle.net/2445/158200
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/toxins12040247
Toxins, 2020, vol. 12, num. 4, p. 247
https://doi.org/10.3390/toxins12040247
info:eu-repo/grantAgreement/EC/H2020/722493
dc.rights.none.fl_str_mv cc-by (c) Filatova, Daria et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Filatova, Daria et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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