Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry

© 2014 American Institute of Chemical Engineers. Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze-dried dairy cattle feces were used (external archaea) as co-inoculum (Co-i) and structural carbohydrates (CHO:...

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Autores: Morazán, H., Seradj, A.R., Alvarez-Rodriguez, J., Abecia, Leticia, Babot, D., Yáñez Ruiz, David R., Balcells, Joaquín
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::c2f32b7cb4b40974f5125e017e1e92b0
Acesso em linha:http://hdl.handle.net/10261/177662
Access Level:acceso abierto
Palavra-chave:DGGE
Slurry
Methane
Archaea
Biodiversity
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spelling Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurryMorazán, H.Seradj, A.R.Alvarez-Rodriguez, J.Abecia, LeticiaBabot, D.Yáñez Ruiz, David R.Balcells, JoaquínDGGESlurryMethaneArchaeaBiodiversity© 2014 American Institute of Chemical Engineers. Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze-dried dairy cattle feces were used (external archaea) as co-inoculum (Co-i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as substrates. Bottles were incubated (39 ± 1°C for 56 days), and the gas production was measured (mbar) and converted to the volume. A sample of produced gas was taken and analyzed for methane concentration using a gas chromatography. Bottles were opened at days 0, 25, and 56 to determine total bacteria, total, and hydrogenotrophic methanogens archaea (HMA) concentrations using quantitative polymerase chain reaction and population biodiversity using denaturing gradient gel electrophoresis (DGGE). Incubation time reduced the titers of total bacteria and archaea (P < 0.01) but did not modify HMA population. Doses of Co-i showed a positive correlation with HMA titers, although interacted with an incubation period (P < 0.001); at 5% Coinoculation, total bacteria decreased significantly (0-25 days) but remained steady until day 56 (P > 0.05), whereas at 10% Co inoculation, titers decreased constantly. Most of the archaeal DGGE bands were observed in all samples, suggesting a common microbial population origin but Co-i supply altered the DGGE structure of archaea populations.This work was supported by the Ministry of Economy and Competitiveness of Spain and the European Union Regional Development Funds (AGL2010‐20820). Ahmad Reza Seradj is a recipient of grant from the Government of Catalunya (FI‐DGR 2011) and Henris Morazan was a recipient of grant from MAEC‐AECID 2009–2011.Peer ReviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)European CommissionMinisterio de Asuntos Exteriores y Cooperación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920152019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/177662reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::c2f32b7cb4b40974f5125e017e1e92b02026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
spellingShingle Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
Morazán, H.
DGGE
Slurry
Methane
Archaea
Biodiversity
title_short Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_full Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_fullStr Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_full_unstemmed Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_sort Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
dc.creator.none.fl_str_mv Morazán, H.
Seradj, A.R.
Alvarez-Rodriguez, J.
Abecia, Leticia
Babot, D.
Yáñez Ruiz, David R.
Balcells, Joaquín
author Morazán, H.
author_facet Morazán, H.
Seradj, A.R.
Alvarez-Rodriguez, J.
Abecia, Leticia
Babot, D.
Yáñez Ruiz, David R.
Balcells, Joaquín
author_role author
author2 Seradj, A.R.
Alvarez-Rodriguez, J.
Abecia, Leticia
Babot, D.
Yáñez Ruiz, David R.
Balcells, Joaquín
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Ministerio de Asuntos Exteriores y Cooperación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv DGGE
Slurry
Methane
Archaea
Biodiversity
topic DGGE
Slurry
Methane
Archaea
Biodiversity
description © 2014 American Institute of Chemical Engineers. Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze-dried dairy cattle feces were used (external archaea) as co-inoculum (Co-i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as substrates. Bottles were incubated (39 ± 1°C for 56 days), and the gas production was measured (mbar) and converted to the volume. A sample of produced gas was taken and analyzed for methane concentration using a gas chromatography. Bottles were opened at days 0, 25, and 56 to determine total bacteria, total, and hydrogenotrophic methanogens archaea (HMA) concentrations using quantitative polymerase chain reaction and population biodiversity using denaturing gradient gel electrophoresis (DGGE). Incubation time reduced the titers of total bacteria and archaea (P < 0.01) but did not modify HMA population. Doses of Co-i showed a positive correlation with HMA titers, although interacted with an incubation period (P < 0.001); at 5% Coinoculation, total bacteria decreased significantly (0-25 days) but remained steady until day 56 (P > 0.05), whereas at 10% Co inoculation, titers decreased constantly. Most of the archaeal DGGE bands were observed in all samples, suggesting a common microbial population origin but Co-i supply altered the DGGE structure of archaea populations.
publishDate 2015
dc.date.none.fl_str_mv 2015
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/177662
url http://hdl.handle.net/10261/177662
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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