Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)

Balbuena-Pecino, Sara; Riera-Heredia, Natàlia; Sánchez-Moya, Albert; Perelló-Amorós, Miquel; Gutiérrez, Joaquim; Capilla, Encarnación; Navarro, Isabel

Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)

Aquafeed formulation has progressively reduced its dependence on fish-derived ingredients over the past decades. Plant-based substitutes have been a major focus, with soybean meal and its derivatives leading the way. However, many plants contain phytoestrogens, which may affect fish physiology. This...

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Autores:	Balbuena-Pecino, Sara, Riera-Heredia, Natàlia, Sánchez-Moya, Albert, Perelló-Amorós, Miquel, Gutiérrez, Joaquim, Capilla, Encarnación, Navarro, Isabel
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2025
País:	España
Institución:	Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:	DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:	oai:digital.csic.es:10261/418526
Acceso en línea:	http://hdl.handle.net/10261/418526 https://api.elsevier.com/content/abstract/scopus_id/105000867572
Access Level:	acceso abierto
Palabra clave:	Phytocompounds Aquaculture Coumestrol Estradiol Fish nutrition Genistein http://metadata.un.org/sdg/17 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 http://metadata.un.org/sdg/11 http://metadata.un.org/sdg/12 http://metadata.un.org/sdg/14 Ensure healthy lives and promote well-being for all at all ages Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Make cities and human settlements inclusive, safe, resilient and sustainable Ensure sustainable consumption and production patterns Conserve and sustainably use the oceans, seas and marine resources for sustainable development Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development

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dc.title.none.fl_str_mv	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
title	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
spellingShingle	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) Balbuena-Pecino, Sara Phytocompounds Aquaculture Coumestrol Estradiol Fish nutrition Genistein http://metadata.un.org/sdg/17 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 http://metadata.un.org/sdg/11 http://metadata.un.org/sdg/12 http://metadata.un.org/sdg/14 Ensure healthy lives and promote well-being for all at all ages Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Make cities and human settlements inclusive, safe, resilient and sustainable Ensure sustainable consumption and production patterns Conserve and sustainably use the oceans, seas and marine resources for sustainable development Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development
title_short	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
title_full	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
title_fullStr	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
title_full_unstemmed	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
title_sort	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)
dc.creator.none.fl_str_mv	Balbuena-Pecino, Sara Riera-Heredia, Natàlia Sánchez-Moya, Albert Perelló-Amorós, Miquel Gutiérrez, Joaquim Capilla, Encarnación Navarro, Isabel
author	Balbuena-Pecino, Sara
author_facet	Balbuena-Pecino, Sara Riera-Heredia, Natàlia Sánchez-Moya, Albert Perelló-Amorós, Miquel Gutiérrez, Joaquim Capilla, Encarnación Navarro, Isabel
author_role	author
author2	Riera-Heredia, Natàlia Sánchez-Moya, Albert Perelló-Amorós, Miquel Gutiérrez, Joaquim Capilla, Encarnación Navarro, Isabel
author2_role	author author author author author author
dc.contributor.none.fl_str_mv	Ministerio de Ciencia, Innovación y Universidades (España) 0000-0001-5101-4814 0000-0002-6695-2325 0000-0002-5749-5978 0000-0002-6776-8765 0000-0002-3126-2236 0000-0002-0863-9728 0000-0002-2641-0361 Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv	Phytocompounds Aquaculture Coumestrol Estradiol Fish nutrition Genistein http://metadata.un.org/sdg/17 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 http://metadata.un.org/sdg/11 http://metadata.un.org/sdg/12 http://metadata.un.org/sdg/14 Ensure healthy lives and promote well-being for all at all ages Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Make cities and human settlements inclusive, safe, resilient and sustainable Ensure sustainable consumption and production patterns Conserve and sustainably use the oceans, seas and marine resources for sustainable development Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development
topic	Phytocompounds Aquaculture Coumestrol Estradiol Fish nutrition Genistein http://metadata.un.org/sdg/17 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 http://metadata.un.org/sdg/11 http://metadata.un.org/sdg/12 http://metadata.un.org/sdg/14 Ensure healthy lives and promote well-being for all at all ages Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Make cities and human settlements inclusive, safe, resilient and sustainable Ensure sustainable consumption and production patterns Conserve and sustainably use the oceans, seas and marine resources for sustainable development Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development
description	Aquafeed formulation has progressively reduced its dependence on fish-derived ingredients over the past decades. Plant-based substitutes have been a major focus, with soybean meal and its derivatives leading the way. However, many plants contain phytoestrogens, which may affect fish physiology. This study aimed to assess in vitro the effects of genistein (GE), daidzein (DZN), glycitein (GLY), and coumestrol (COU) on the lipid metabolism of rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata). Primary cultured adipocytes were incubated with these phytoestrogens, along with 17β-estradiol, at two doses each (1, 10, or 100 μM). The 100 μM dose of GE and DZN decreased adipocyte viability, and mainly enhanced lipid accumulation in both species, suggesting a hypertrophic condition. However, the reduction in adipocyte number and lipid content with 100 μM DZN in rainbow trout indicated a limiting effect on adipose tissue growth in this species. Interestingly, COU significantly increased cell viability in gilthead sea bream, potentially leading to hyperplastic growth, a more favorable metabolic state. In that species, which proved to be more phytoestrogens-sensitive, lipoprotein lipase was generally downregulated upon treatments. Moreover, 10 µM GE significantly decreased the mRNA levels of fatty acid transport protein 1 and fatty acid synthase, and increased those of fatty acid binding protein 1, suggesting an acceleration of the differentiation process compared to the control cells. This work provides new insights into how dietary phytoestrogens modulate fish lipid metabolism and supports that their presence in plant protein feedstuffs can potentially affect fish health and production performance.
publishDate	2025
dc.date.none.fl_str_mv	2025 2026 2026
dc.type.none.fl_str_mv	info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10261/418526 https://api.elsevier.com/content/abstract/scopus_id/105000867572
url	http://hdl.handle.net/10261/418526 https://api.elsevier.com/content/abstract/scopus_id/105000867572
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICIU/AEI/10.13039 Fish physiology and biochemistry https://doi.org/10.1007/s10695-025-01483-1 Sí
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Springer Nature
publisher.none.fl_str_mv	Springer Nature
dc.source.none.fl_str_mv	reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str	Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str	DIGITAL.CSIC. Repositorio Institucional del CSIC
collection	DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869404855196450816
spelling	Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)Balbuena-Pecino, SaraRiera-Heredia, NatàliaSánchez-Moya, AlbertPerelló-Amorós, MiquelGutiérrez, JoaquimCapilla, EncarnaciónNavarro, IsabelPhytocompoundsAquacultureCoumestrolEstradiolFish nutritionGenisteinhttp://metadata.un.org/sdg/17http://metadata.un.org/sdg/9http://metadata.un.org/sdg/3http://metadata.un.org/sdg/11http://metadata.un.org/sdg/12http://metadata.un.org/sdg/14Ensure healthy lives and promote well-being for all at all agesBuild resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationMake cities and human settlements inclusive, safe, resilient and sustainableEnsure sustainable consumption and production patternsConserve and sustainably use the oceans, seas and marine resources for sustainable developmentStrengthen the means of implementation and revitalize the Global Partnership for Sustainable DevelopmentAquafeed formulation has progressively reduced its dependence on fish-derived ingredients over the past decades. Plant-based substitutes have been a major focus, with soybean meal and its derivatives leading the way. However, many plants contain phytoestrogens, which may affect fish physiology. This study aimed to assess in vitro the effects of genistein (GE), daidzein (DZN), glycitein (GLY), and coumestrol (COU) on the lipid metabolism of rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata). Primary cultured adipocytes were incubated with these phytoestrogens, along with 17β-estradiol, at two doses each (1, 10, or 100 μM). The 100 μM dose of GE and DZN decreased adipocyte viability, and mainly enhanced lipid accumulation in both species, suggesting a hypertrophic condition. However, the reduction in adipocyte number and lipid content with 100 μM DZN in rainbow trout indicated a limiting effect on adipose tissue growth in this species. Interestingly, COU significantly increased cell viability in gilthead sea bream, potentially leading to hyperplastic growth, a more favorable metabolic state. In that species, which proved to be more phytoestrogens-sensitive, lipoprotein lipase was generally downregulated upon treatments. Moreover, 10 µM GE significantly decreased the mRNA levels of fatty acid transport protein 1 and fatty acid synthase, and increased those of fatty acid binding protein 1, suggesting an acceleration of the differentiation process compared to the control cells. This work provides new insights into how dietary phytoestrogens modulate fish lipid metabolism and supports that their presence in plant protein feedstuffs can potentially affect fish health and production performance.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study is part of the R + D + i projects AGL2017-89436-R and PID2020-116172RBI00 to E.C. and I.N., funded by MICIU/AEI/10.13039/501100011033. It was also supported by the grants 2017SGR-1574 and 2021SGR-00713, from the “Generalitat de Catalunya”. S.B.-P., N.R.-H., and M.P.-A. received support through predoctoral fellowships from the MICIU (PRE2018-085580, BES-2015–074654 and BES-2016–078697, respectively).Peer reviewedSpringer NatureMinisterio de Ciencia, Innovación y Universidades (España)0000-0001-5101-48140000-0002-6695-23250000-0002-5749-59780000-0002-6776-87650000-0002-3126-22360000-0002-0863-97280000-0002-2641-0361Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/418526https://api.elsevier.com/content/abstract/scopus_id/105000867572reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICIU/AEI/10.13039Fish physiology and biochemistryhttps://doi.org/10.1007/s10695-025-01483-1Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4185262026-05-22T06:33:51Z
score	15,811543

Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)

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