Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect

[EN] Plasma membrane and membranous organelles contribute to the physiology of the Eukaryotic cell by participating in vesicle trafficking and the maintenance of ion homeostasis. Exomer is a protein complex that facilitates vesicle transport from the trans-Golgi network to the plasma membrane, and i...

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Autores: Moro, Sandra, Moscoso-Romero, Esteban, Poddar, Abhishek, Pérez, Pilar, Chen, Qian, Valdivieso, M.-Henar, Mulet, José Miguel|||0000-0002-9087-3838
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositório:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglês
OAI Identifier:oai:riunet.upv.es:10251/183406
Acesso em linha:https://riunet.upv.es/handle/10251/183406
Access Level:Acceso aberto
Palavra-chave:Endosome
Exomer
Ionic stress
Ion homeostasis
Potassium
Secretion
Vesicle traffic
Yeast
BIOQUIMICA Y BIOLOGIA MOLECULAR
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
07.- Asegurar el acceso a energías asequibles, fiables, sostenibles y modernas para todos
09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación
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oai_identifier_str oai:riunet.upv.es:10251/183406
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
title Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
spellingShingle Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
Moro, Sandra
Endosome
Exomer
Ionic stress
Ion homeostasis
Potassium
Secretion
Vesicle traffic
Yeast
BIOQUIMICA Y BIOLOGIA MOLECULAR
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
07.- Asegurar el acceso a energías asequibles, fiables, sostenibles y modernas para todos
09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación
title_short Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
title_full Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
title_fullStr Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
title_full_unstemmed Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
title_sort Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect
dc.creator.none.fl_str_mv Moro, Sandra
Moscoso-Romero, Esteban
Poddar, Abhishek
Pérez, Pilar
Chen, Qian
Valdivieso, M.-Henar
Mulet, José Miguel|||0000-0002-9087-3838
author Moro, Sandra
author_facet Moro, Sandra
Moscoso-Romero, Esteban
Poddar, Abhishek
Pérez, Pilar
Chen, Qian
Valdivieso, M.-Henar
Mulet, José Miguel|||0000-0002-9087-3838
author_role author
author2 Moscoso-Romero, Esteban
Poddar, Abhishek
Pérez, Pilar
Chen, Qian
Valdivieso, M.-Henar
Mulet, José Miguel|||0000-0002-9087-3838
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Educación
Universidad de Salamanca
Junta de Castilla y León
AGENCIA ESTATAL DE INVESTIGACION
Agencia Estatal de Investigación
European Regional Development Fund
National Institutes of Health, EEUU
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Endosome
Exomer
Ionic stress
Ion homeostasis
Potassium
Secretion
Vesicle traffic
Yeast
BIOQUIMICA Y BIOLOGIA MOLECULAR
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
07.- Asegurar el acceso a energías asequibles, fiables, sostenibles y modernas para todos
09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación
topic Endosome
Exomer
Ionic stress
Ion homeostasis
Potassium
Secretion
Vesicle traffic
Yeast
BIOQUIMICA Y BIOLOGIA MOLECULAR
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
07.- Asegurar el acceso a energías asequibles, fiables, sostenibles y modernas para todos
09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación
description [EN] Plasma membrane and membranous organelles contribute to the physiology of the Eukaryotic cell by participating in vesicle trafficking and the maintenance of ion homeostasis. Exomer is a protein complex that facilitates vesicle transport from the trans-Golgi network to the plasma membrane, and its absence leads to the retention of a set of selected cargoes in this organelle. However, this retention does not explain all phenotypes observed in exomer mutants. The Schizosaccharomyces pombe exomer is composed of Cfr1 and Bch1, and cfr1¿ and bch1¿ were sensitive to high concentrations of potassium salts but not sorbitol, which showed sensitivity to ionic but not osmotic stress. Additionally, the activity of the plasma membrane ATPase was higher in exomer mutants than in the wild-type, pointing to membrane hyperpolarization, which caused an increase in intracellular K+ content and mild sensitivity to Na+, Ca2+, and the aminoglycoside antibiotic hygromycin B. Moreover, in response to K+ shock, the intracellular Ca2+ level of cfr1¿ cells increased significantly more than in the wild-type, likely due to the larger Ca2+ spikes in the mutant. Microscopy analyses showed a defective endosomal morphology in the mutants. This was accompanied by an increase in the intracellular pools of the K+ exporting P-type ATPase Cta3 and the plasma membrane Transient Receptor Potential (TRP)-like Ca2+ channel Pkd2, which were partially diverted from the trans-Golgi network to the prevacuolar endosome. Despite this, most Cta3 and Pkd2 were delivered to the plasma membrane at the cell growing sites, showing that their transport from the trans-Golgi network to the cell surface occurred in the absence of exomer. Nevertheless, shortly after gene expression in the presence of KCl, the polarized distribution of Cta3 and Pkd2 in the plasma membrane was disturbed in the mutants. Finally, the use of fluorescent probes suggested that the distribution and dynamics of association of some lipids to the plasma membrane in the presence of KCl were altered in the mutants. Thus, exomer participation in the response to K+ stress was multifaceted. These results supported the notion that exomer plays a general role in protein sorting at the trans-Golgi network and in polarized secretion, which is not always related to a function as a selective cargo adaptor.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-19
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/183406
url https://riunet.upv.es/handle/10251/183406
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-84508-P LAS LEVADURAS COMO MODELO PARA EL ESTUDIO DE LA CLASIFICACION PROTEICA EN EL TGN: FUNCION DEL EXOMERO
Junta de Castilla y León https://doi.org/10.13039/501100014180 CSI150P20
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-098924-B-I00 CITOCINESIS E INTEGRIDAD CELULAR EN LA CELULA FUNGICA Y SU INTERES EN TERAPIAS ANTIFUNGICAS
Junta de Castilla y León https://doi.org/10.13039/501100014180 CLU-2017-03%2F14-20
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-104054GB-I00 ESTUDIO DE REGULADORES DE CANALES DE POTASIO EN EL CONTROL DE LA APERTURA ESTOMATICA Y DE RESISTENCIA A SEQUIA
National Institutes of Health, EEUU https://doi.org/10.13039/100000002 R15GM134496
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 RTC-2017-6468-2-AR APROXIMACIONES MOLECULARES PARA INCREMENTAR LA TOLERANCIA A SALINIDAD Y SEQUIA DEL BROCOLI
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress ConnectMoro, SandraMoscoso-Romero, EstebanPoddar, AbhishekPérez, PilarChen, QianValdivieso, M.-HenarMulet, José Miguel|||0000-0002-9087-3838EndosomeExomerIonic stressIon homeostasisPotassiumSecretionVesicle trafficYeastBIOQUIMICA Y BIOLOGIA MOLECULAR03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades07.- Asegurar el acceso a energías asequibles, fiables, sostenibles y modernas para todos09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación[EN] Plasma membrane and membranous organelles contribute to the physiology of the Eukaryotic cell by participating in vesicle trafficking and the maintenance of ion homeostasis. Exomer is a protein complex that facilitates vesicle transport from the trans-Golgi network to the plasma membrane, and its absence leads to the retention of a set of selected cargoes in this organelle. However, this retention does not explain all phenotypes observed in exomer mutants. The Schizosaccharomyces pombe exomer is composed of Cfr1 and Bch1, and cfr1¿ and bch1¿ were sensitive to high concentrations of potassium salts but not sorbitol, which showed sensitivity to ionic but not osmotic stress. Additionally, the activity of the plasma membrane ATPase was higher in exomer mutants than in the wild-type, pointing to membrane hyperpolarization, which caused an increase in intracellular K+ content and mild sensitivity to Na+, Ca2+, and the aminoglycoside antibiotic hygromycin B. Moreover, in response to K+ shock, the intracellular Ca2+ level of cfr1¿ cells increased significantly more than in the wild-type, likely due to the larger Ca2+ spikes in the mutant. Microscopy analyses showed a defective endosomal morphology in the mutants. This was accompanied by an increase in the intracellular pools of the K+ exporting P-type ATPase Cta3 and the plasma membrane Transient Receptor Potential (TRP)-like Ca2+ channel Pkd2, which were partially diverted from the trans-Golgi network to the prevacuolar endosome. Despite this, most Cta3 and Pkd2 were delivered to the plasma membrane at the cell growing sites, showing that their transport from the trans-Golgi network to the cell surface occurred in the absence of exomer. Nevertheless, shortly after gene expression in the presence of KCl, the polarized distribution of Cta3 and Pkd2 in the plasma membrane was disturbed in the mutants. Finally, the use of fluorescent probes suggested that the distribution and dynamics of association of some lipids to the plasma membrane in the presence of KCl were altered in the mutants. Thus, exomer participation in the response to K+ stress was multifaceted. These results supported the notion that exomer plays a general role in protein sorting at the trans-Golgi network and in polarized secretion, which is not always related to a function as a selective cargo adaptor.Financial support from the Spanish Ministerio de Economía, Industria y Competitividad/European Regional Development Fund¿FEDER¿program (BFU2017-84508-P to M-HV and PGC2018-098924-B-I00 to PP), and from the Junta de Castilla y Leon/FEDER program (CSI150P20 to PP, and ¿Escalera de Excelencia¿ CLU-2017-03/14-20 to the IBFG), made this work possible. SM was supported by fellowships from the Universidad de Salamanca and the Spanish Ministerio de Educación, and EMR by a fellowship from Junta de Castilla y León. The Agencia Española de Investigación (AEI) Grants PID2019-104054 GB-I00 and RTC-2017-6468-2-AR supported JM. QC and AP were supported by the University of Toledo start up fund and a grant from National Institutes of Health (R15GM134496).Frontiers Media SAInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de EducaciónUniversidad de SalamancaJunta de Castilla y LeónAGENCIA ESTATAL DE INVESTIGACIONAgencia Estatal de InvestigaciónEuropean Regional Development FundNational Institutes of Health, EEUURepositorio Institucional de la Universitat Politècnica de València Riunet20212021-07-19journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/183406reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-84508-P LAS LEVADURAS COMO MODELO PARA EL ESTUDIO DE LA CLASIFICACION PROTEICA EN EL TGN: FUNCION DEL EXOMEROJunta de Castilla y León https://doi.org/10.13039/501100014180 CSI150P20Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-098924-B-I00 CITOCINESIS E INTEGRIDAD CELULAR EN LA CELULA FUNGICA Y SU INTERES EN TERAPIAS ANTIFUNGICASJunta de Castilla y León https://doi.org/10.13039/501100014180 CLU-2017-03%2F14-20Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-104054GB-I00 ESTUDIO DE REGULADORES DE CANALES DE POTASIO EN EL CONTROL DE LA APERTURA ESTOMATICA Y DE RESISTENCIA A SEQUIANational Institutes of Health, EEUU https://doi.org/10.13039/100000002 R15GM134496Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 RTC-2017-6468-2-AR APROXIMACIONES MOLECULARES PARA INCREMENTAR LA TOLERANCIA A SALINIDAD Y SEQUIA DEL BROCOLIopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1834062026-06-13T07:49:27Z
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