High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation

Optimization of protocols is required for each specific type of biomass processed by electroporation of the cell membrane with high voltage pulsed electric fields (PEF). Such optimization requires convenient and adaptable laboratory systems, which will enable determination of both electrical and mec...

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Detalhes bibliográficos
Autores: Levkov, Klimentiy, Linzon, Yoav, Mercadal, Borja, Ivorra Cano, Antoni, 1974-, González, César Antonio, Golberg, Alexander
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/43887
Acesso em linha:http://hdl.handle.net/10230/43887
http://dx.doi.org/10.1016/j.ifset.2020.102288
Access Level:acceso abierto
Palavra-chave:Pulsed electric field generator
Electroporation
Biomass processing
Macroalgae
Bioimpedance
Bioeconomy
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spelling High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporationLevkov, KlimentiyLinzon, YoavMercadal, BorjaIvorra Cano, Antoni, 1974-González, César AntonioGolberg, AlexanderPulsed electric field generatorElectroporationBiomass processingMacroalgaeBioimpedanceBioeconomyOptimization of protocols is required for each specific type of biomass processed by electroporation of the cell membrane with high voltage pulsed electric fields (PEF). Such optimization requires convenient and adaptable laboratory systems, which will enable determination of both electrical and mechanical parameters for successful electroporation and fractionation. In this work, we report on a laboratory PEF system consisting of a high voltage generator with a novel asymmetric voltage multiplying architecture and a treatment chamber with sliding electrodes. The system allows applying pulses of up to 4 kV and 1 kA with a pulse duration between 1 μs and 100 μs. The allowable energy dissipated per pulse on electroporated biomass is determined by the conditions for cooling the biomass in the electroporation cell. The device was tested on highly conductive green macroalgae from Ulva sp., a promising but challenging feedstock for the biorefinery. Successful electroporation was confirmed with bioimpedance measurements.Elsevier20202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/43887http://dx.doi.org/10.1016/j.ifset.2020.102288reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInnovative Food Science & Emerging Technologies. 2020 Mar;60:102288© Elsevier http://dx.doi.org/10.1016/j.ifset.2020.102288info:eu-repo/semantics/openAccessoai:recercat.cat:10230/438872026-05-29T05:05:01Z
dc.title.none.fl_str_mv High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
title High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
spellingShingle High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
Levkov, Klimentiy
Pulsed electric field generator
Electroporation
Biomass processing
Macroalgae
Bioimpedance
Bioeconomy
title_short High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
title_full High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
title_fullStr High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
title_full_unstemmed High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
title_sort High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
dc.creator.none.fl_str_mv Levkov, Klimentiy
Linzon, Yoav
Mercadal, Borja
Ivorra Cano, Antoni, 1974-
González, César Antonio
Golberg, Alexander
author Levkov, Klimentiy
author_facet Levkov, Klimentiy
Linzon, Yoav
Mercadal, Borja
Ivorra Cano, Antoni, 1974-
González, César Antonio
Golberg, Alexander
author_role author
author2 Linzon, Yoav
Mercadal, Borja
Ivorra Cano, Antoni, 1974-
González, César Antonio
Golberg, Alexander
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pulsed electric field generator
Electroporation
Biomass processing
Macroalgae
Bioimpedance
Bioeconomy
topic Pulsed electric field generator
Electroporation
Biomass processing
Macroalgae
Bioimpedance
Bioeconomy
description Optimization of protocols is required for each specific type of biomass processed by electroporation of the cell membrane with high voltage pulsed electric fields (PEF). Such optimization requires convenient and adaptable laboratory systems, which will enable determination of both electrical and mechanical parameters for successful electroporation and fractionation. In this work, we report on a laboratory PEF system consisting of a high voltage generator with a novel asymmetric voltage multiplying architecture and a treatment chamber with sliding electrodes. The system allows applying pulses of up to 4 kV and 1 kA with a pulse duration between 1 μs and 100 μs. The allowable energy dissipated per pulse on electroporated biomass is determined by the conditions for cooling the biomass in the electroporation cell. The device was tested on highly conductive green macroalgae from Ulva sp., a promising but challenging feedstock for the biorefinery. Successful electroporation was confirmed with bioimpedance measurements.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/43887
http://dx.doi.org/10.1016/j.ifset.2020.102288
url http://hdl.handle.net/10230/43887
http://dx.doi.org/10.1016/j.ifset.2020.102288
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Innovative Food Science & Emerging Technologies. 2020 Mar;60:102288
dc.rights.none.fl_str_mv © Elsevier http://dx.doi.org/10.1016/j.ifset.2020.102288
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © Elsevier http://dx.doi.org/10.1016/j.ifset.2020.102288
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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