Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus

21 Pág.

Detalles Bibliográficos
Autores: Perdiguero, Pedro, Jiménez-Barrios, Pablo, Morel, Esther, Abós, Beatriz, Tafalla, Carolina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/369250
Acceso en línea:http://hdl.handle.net/10261/369250
https://api.elsevier.com/content/abstract/scopus_id/85198944591
Access Level:acceso abierto
Palabra clave:B cells
Infectious pancreatic necrosis virus (IPNV)
Peripheral blood leukocytes (PBLs)
Single cell transcriptomics
Teleost
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dc.title.none.fl_str_mv Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
title Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
spellingShingle Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
Perdiguero, Pedro
B cells
Infectious pancreatic necrosis virus (IPNV)
Peripheral blood leukocytes (PBLs)
Single cell transcriptomics
Teleost
title_short Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
title_full Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
title_fullStr Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
title_full_unstemmed Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
title_sort Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virus
dc.creator.none.fl_str_mv Perdiguero, Pedro
Jiménez-Barrios, Pablo
Morel, Esther
Abós, Beatriz
Tafalla, Carolina
author Perdiguero, Pedro
author_facet Perdiguero, Pedro
Jiménez-Barrios, Pablo
Morel, Esther
Abós, Beatriz
Tafalla, Carolina
author_role author
author2 Jiménez-Barrios, Pablo
Morel, Esther
Abós, Beatriz
Tafalla, Carolina
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Comunidad de Madrid
Agencia Estatal de Investigación (España)
Perdiguero, Pedro [0000-0002-5471-9550]
Jiménez-Barrios, Pablo [0000-0001-8288-5292]
Morel, Esther [0000-0002-4768-082X]
Abós, Beatriz [0000-0002-3402-7397]
Tafalla, Carolina [0000-0002-0860-2976]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv B cells
Infectious pancreatic necrosis virus (IPNV)
Peripheral blood leukocytes (PBLs)
Single cell transcriptomics
Teleost
topic B cells
Infectious pancreatic necrosis virus (IPNV)
Peripheral blood leukocytes (PBLs)
Single cell transcriptomics
Teleost
description 21 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/369250
https://api.elsevier.com/content/abstract/scopus_id/85198944591
url http://hdl.handle.net/10261/369250
https://api.elsevier.com/content/abstract/scopus_id/85198944591
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/EC/725061/
S2016-T1/BIO-1672
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113268RB-I00
Centro de Investigación en Sanidad Animal (CISA)
https://doi.org/10.3389/fimmu.2024.1404209
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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spelling Single-cell atlas of rainbow trout peripheral blood leukocytes and profiling of their early response to infectious pancreatic necrosis virusPerdiguero, PedroJiménez-Barrios, PabloMorel, EstherAbós, BeatrizTafalla, CarolinaB cellsInfectious pancreatic necrosis virus (IPNV)Peripheral blood leukocytes (PBLs)Single cell transcriptomicsTeleost21 Pág.The recent development of single cell sequencing technologies has revolutionized the state-of-art of cell biology, allowing the simultaneous measurement of thousands of genes in single cells. This technology has been applied to study the transcriptome of single cells in homeostasis and also in response to pathogenic exposure, greatly increasing our knowledge of the immune response to infectious agents. Yet the number of these studies performed in aquacultured fish species is still very limited. Thus, in the current study, we have used the 10x Genomics single cell RNA sequencing technology to study the response of rainbow trout (Oncorhynchus mykiss) peripheral blood leukocytes (PBLs) to infectious pancreatic necrosis virus (IPNV), an important trout pathogen. The study allowed us to obtain a transcriptomic profile of 12 transcriptionally distinct leukocyte cell subpopulations that included four different subsets of B cells, T cells, monocytes, two populations of dendritic-like cells (DCs), hematopoietic progenitor cells, non-specific cytotoxic cells (NCC), neutrophils and thrombocytes. The transcriptional pattern of these leukocyte subpopulations was compared in PBL cultures that had been exposed in vitro to IPNV for 24 h and mock-infected cultures. Our results revealed that monocytes and neutrophils showed the highest number of upregulated protein-coding genes in response to IPNV. Interestingly, IgM+IgD+ and IgT+ B cells also upregulated an important number of genes to the virus, but a much fainter response was observed in ccl4 + or plasma-like cells (irf4 + cells). A substantial number of protein-coding genes and genes coding for ribosomal proteins were also transcriptionally upregulated in response to IPNV in T cells and thrombocytes. Interestingly, although genes coding for ribosomal proteins were regulated in all affected PBL subpopulations, the number of such genes transcriptionally regulated was higher in IgM+IgD+ and IgT+ B cells. A further analysis dissected which of the regulated genes were common and which were specific to the different cell clusters, identifying eight genes that were transcriptionally upregulated in all the affected groups. The data provided constitutes a comprehensive transcriptional perspective of how the different leukocyte populations present in blood respond to an early viral encounter in fish.The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM) and by the Comunidad de Madrid (grant 2016-T1/BIO-1672). This work was also part of the project PID2020-113268RB-I00 funded by MCNI/AEI/10.13039/501100011033.Peer reviewedFrontiers MediaEuropean CommissionComunidad de MadridAgencia Estatal de Investigación (España)Perdiguero, Pedro [0000-0002-5471-9550]Jiménez-Barrios, Pablo [0000-0001-8288-5292]Morel, Esther [0000-0002-4768-082X]Abós, Beatriz [0000-0002-3402-7397]Tafalla, Carolina [0000-0002-0860-2976]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/369250https://api.elsevier.com/content/abstract/scopus_id/85198944591reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/725061/S2016-T1/BIO-1672info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113268RB-I00Centro de Investigación en Sanidad Animal (CISA)https://doi.org/10.3389/fimmu.2024.1404209Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3692502026-05-22T06:33:51Z
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