miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2

Age related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of...

Descripción completa

Detalles Bibliográficos
Autores: Oltra Sanchis, María, Vidal Gil, Lorena, Maisto, Rosa, Oltra, Sara S., Romero, Francisco Javier, Sancho Pelluz, Francisco Javier, Barcia González, Jorge Miguel
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/3665
Acceso en línea:http://hdl.handle.net/20.500.12466/3665
Access Level:acceso abierto
Palabra clave:miR302a
2409 Genética
3201.09 Oftalmología
id ES_2683b71df063e04578aee0841b3dd939
oai_identifier_str oai:riucv.ucv.es:20.500.12466/3665
network_acronym_str ES
network_name_str España
repository_id_str
spelling miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2Oltra Sanchis, MaríaVidal Gil, LorenaMaisto, RosaOltra, Sara S.Romero, Francisco JavierSancho Pelluz, Francisco JavierBarcia González, Jorge MiguelmiR302a2409 Genética3201.09 OftalmologíaAge related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of circulating miRNAs is opening new possibilities in terms of diagnosis and therapeutics. miRNAs can travel associated to lipoproteins or inside small Extracellular Vesicles (sEVs). A number of reports indicate a signifcant deregulation of circulating miRNAs in AMD and experimental approaches, but it is unclear whether sEVs present a signifcant miRNA cargo. The present work studies miRNA expression changes in sEVs released from ARPE-19 cells under oxidative conditions (i.e. hydrogen peroxide, H2O2). H2O2 increased sEVs release from ARPE-19 cells. Moreover, 218 miRNAs could be detected in control and H2O2 induced-sEVs. Interestingly, only two of them (hsa-miR-302a and hsa-miR-122) were signifcantly under-expressed in H2O2-induced sEVs. Results herein suggest that the down regulation of miRNAs 302a and 122 might be related with previous studies showing sEVs-induced neovascularization after oxidative challenge in ARPE-19 cells.20242024-01-1720192019-11-2920192019-11-29journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/3665reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/36652026-06-19T08:32:07Z
dc.title.none.fl_str_mv miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
title miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
spellingShingle miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
Oltra Sanchis, María
miR302a
2409 Genética
3201.09 Oftalmología
title_short miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
title_full miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
title_fullStr miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
title_full_unstemmed miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
title_sort miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2
dc.creator.none.fl_str_mv Oltra Sanchis, María
Vidal Gil, Lorena
Maisto, Rosa
Oltra, Sara S.
Romero, Francisco Javier
Sancho Pelluz, Francisco Javier
Barcia González, Jorge Miguel
author Oltra Sanchis, María
author_facet Oltra Sanchis, María
Vidal Gil, Lorena
Maisto, Rosa
Oltra, Sara S.
Romero, Francisco Javier
Sancho Pelluz, Francisco Javier
Barcia González, Jorge Miguel
author_role author
author2 Vidal Gil, Lorena
Maisto, Rosa
Oltra, Sara S.
Romero, Francisco Javier
Sancho Pelluz, Francisco Javier
Barcia González, Jorge Miguel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv miR302a
2409 Genética
3201.09 Oftalmología
topic miR302a
2409 Genética
3201.09 Oftalmología
description Age related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of circulating miRNAs is opening new possibilities in terms of diagnosis and therapeutics. miRNAs can travel associated to lipoproteins or inside small Extracellular Vesicles (sEVs). A number of reports indicate a signifcant deregulation of circulating miRNAs in AMD and experimental approaches, but it is unclear whether sEVs present a signifcant miRNA cargo. The present work studies miRNA expression changes in sEVs released from ARPE-19 cells under oxidative conditions (i.e. hydrogen peroxide, H2O2). H2O2 increased sEVs release from ARPE-19 cells. Moreover, 218 miRNAs could be detected in control and H2O2 induced-sEVs. Interestingly, only two of them (hsa-miR-302a and hsa-miR-122) were signifcantly under-expressed in H2O2-induced sEVs. Results herein suggest that the down regulation of miRNAs 302a and 122 might be related with previous studies showing sEVs-induced neovascularization after oxidative challenge in ARPE-19 cells.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-11-29
2019
2019-11-29
2024
2024-01-17
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/3665
url http://hdl.handle.net/20.500.12466/3665
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404826522091520
score 15.301603

Similares en LA Referencia