Partially coherent optical diffraction tomography toward practical cell study

Optical diffraction tomography (ODT) is a computational imaging technique based on refractive index (RI) contrast. Its application for microscopic imaging of weakly absorbing and scattering samples has been demonstrated by using a specially designed holographic microscope with angular scanning of th...

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Detalles Bibliográficos
Autores: Soto Rueda, Juan Manuel, Rodrigo Martín-Romo, José Augusto, Alieva Krasheninnikova, Tatiana
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/8339
Acceso en línea:https://hdl.handle.net/20.500.14352/8339
Access Level:acceso abierto
Palabra clave:535
Illumination
Dynamics
Rotation
Óptica (Física)
2209.19 Óptica Física
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oai_identifier_str oai:docta.ucm.es:20.500.14352/8339
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repository_id_str
spelling Partially coherent optical diffraction tomography toward practical cell studySoto Rueda, Juan ManuelRodrigo Martín-Romo, José AugustoAlieva Krasheninnikova, Tatiana535IlluminationDynamicsRotationÓptica (Física)2209.19 Óptica FísicaOptical diffraction tomography (ODT) is a computational imaging technique based on refractive index (RI) contrast. Its application for microscopic imaging of weakly absorbing and scattering samples has been demonstrated by using a specially designed holographic microscope with angular scanning of the coherent sample illumination direction. Recently, an alternative low cost technique based on partially coherent sample illumination (PC-ODT), which is compatible with the conventional wide-field transmission microscope, has been established. In this case, the 3D refractive index distribution of the sample is obtained by deconvolution from a single stack of through-focus intensity images. The performance of PC-ODT has been successfully tested on various fixed specimens (diatom frustule and biological cells) and moving bacteria. Here, we demonstrate that the PC-ODT is an efficient tool for the analysis of living eukaryotic cell dynamics at short- and long-term periods. The COS-7 cells, which hail from the African green monkey kidney, have been chosen for this study. A fast data acquisition setup comprising an optical scanning module can be easily attached to the microscope, and it allows observing cell 3D organelle movements and RI variations, with the required temporal resolution. In particular, a more rapid nucleoli rotation than previously reported has been found. The long-term cell monitoring during necrosis reveals significant changes in cell dry mass concentration obtained from recovered RI contrast.Frontiers Media S.AUniversidad Complutense de Madrid20212021-06-2120212021-06-21journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/8339reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/83392026-06-02T12:44:21Z
dc.title.none.fl_str_mv Partially coherent optical diffraction tomography toward practical cell study
title Partially coherent optical diffraction tomography toward practical cell study
spellingShingle Partially coherent optical diffraction tomography toward practical cell study
Soto Rueda, Juan Manuel
535
Illumination
Dynamics
Rotation
Óptica (Física)
2209.19 Óptica Física
title_short Partially coherent optical diffraction tomography toward practical cell study
title_full Partially coherent optical diffraction tomography toward practical cell study
title_fullStr Partially coherent optical diffraction tomography toward practical cell study
title_full_unstemmed Partially coherent optical diffraction tomography toward practical cell study
title_sort Partially coherent optical diffraction tomography toward practical cell study
dc.creator.none.fl_str_mv Soto Rueda, Juan Manuel
Rodrigo Martín-Romo, José Augusto
Alieva Krasheninnikova, Tatiana
author Soto Rueda, Juan Manuel
author_facet Soto Rueda, Juan Manuel
Rodrigo Martín-Romo, José Augusto
Alieva Krasheninnikova, Tatiana
author_role author
author2 Rodrigo Martín-Romo, José Augusto
Alieva Krasheninnikova, Tatiana
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 535
Illumination
Dynamics
Rotation
Óptica (Física)
2209.19 Óptica Física
topic 535
Illumination
Dynamics
Rotation
Óptica (Física)
2209.19 Óptica Física
description Optical diffraction tomography (ODT) is a computational imaging technique based on refractive index (RI) contrast. Its application for microscopic imaging of weakly absorbing and scattering samples has been demonstrated by using a specially designed holographic microscope with angular scanning of the coherent sample illumination direction. Recently, an alternative low cost technique based on partially coherent sample illumination (PC-ODT), which is compatible with the conventional wide-field transmission microscope, has been established. In this case, the 3D refractive index distribution of the sample is obtained by deconvolution from a single stack of through-focus intensity images. The performance of PC-ODT has been successfully tested on various fixed specimens (diatom frustule and biological cells) and moving bacteria. Here, we demonstrate that the PC-ODT is an efficient tool for the analysis of living eukaryotic cell dynamics at short- and long-term periods. The COS-7 cells, which hail from the African green monkey kidney, have been chosen for this study. A fast data acquisition setup comprising an optical scanning module can be easily attached to the microscope, and it allows observing cell 3D organelle movements and RI variations, with the required temporal resolution. In particular, a more rapid nucleoli rotation than previously reported has been found. The long-term cell monitoring during necrosis reveals significant changes in cell dry mass concentration obtained from recovered RI contrast.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-06-21
2021
2021-06-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/8339
url https://hdl.handle.net/20.500.14352/8339
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A
publisher.none.fl_str_mv Frontiers Media S.A
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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