A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model
The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological process...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/221383 |
| Acceso en línea: | http://hdl.handle.net/10261/221383 |
| Access Level: | acceso abierto |
| Palabra clave: | Akirin Subolesin Art Yeast two-hybrid Evolution Music Vaccine Quantum vaccinomics |
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A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin modelArtigas-Jerónimo, SaraPastor Comín, Juan J.Villar, MargaritaContreras, MarinelaAlberdi, PilarLeón Viera, IsraelSoto, LeandroCordero, RaúlValdés, James J.Cabezas-Cruz, AlejandroEstrada-Peña, AgustínFuente, José de laAkirinSubolesinArtYeast two-hybridEvolutionMusicVaccineQuantum vaccinomicsThe main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-κB pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR–protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-κB. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2–protein interactions in the regulation of the NF-κB pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB.This work was financially supported by the Ministerio de Economía, Industria y Competitividad (Spain) grant BFU2016-79892-P. M.V. was funded by the University of Castilla La Mancha, Spain.Peer reviewedMultidisciplinary Digital Publishing InstituteUniversidad de Castilla La ManchaMinisterio de Economía y Competitividad (España)Artigas-Jerónimo, Sara [0000-0002-0068-5738]Villar, Margarita [0000-0003-4172-9079]Contreras, Marinela [0000-0002-1505-5624]Alberdi, Pilar [0000-0002-6999-9735]Cabezas-Cruz, Alejandro [0000-0002-8660-730X]Fuente, José de la [0000-0001-7383-9649]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/221383reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-79892-Phttps://doi.org/10.3390/vaccines8010077Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2213832026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| title |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| spellingShingle |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model Artigas-Jerónimo, Sara Akirin Subolesin Art Yeast two-hybrid Evolution Music Vaccine Quantum vaccinomics |
| title_short |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| title_full |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| title_fullStr |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| title_full_unstemmed |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| title_sort |
A novel combined scientific and artistic approach for the advanced characterization of interactomes: The Akirin/Subolesin model |
| dc.creator.none.fl_str_mv |
Artigas-Jerónimo, Sara Pastor Comín, Juan J. Villar, Margarita Contreras, Marinela Alberdi, Pilar León Viera, Israel Soto, Leandro Cordero, Raúl Valdés, James J. Cabezas-Cruz, Alejandro Estrada-Peña, Agustín Fuente, José de la |
| author |
Artigas-Jerónimo, Sara |
| author_facet |
Artigas-Jerónimo, Sara Pastor Comín, Juan J. Villar, Margarita Contreras, Marinela Alberdi, Pilar León Viera, Israel Soto, Leandro Cordero, Raúl Valdés, James J. Cabezas-Cruz, Alejandro Estrada-Peña, Agustín Fuente, José de la |
| author_role |
author |
| author2 |
Pastor Comín, Juan J. Villar, Margarita Contreras, Marinela Alberdi, Pilar León Viera, Israel Soto, Leandro Cordero, Raúl Valdés, James J. Cabezas-Cruz, Alejandro Estrada-Peña, Agustín Fuente, José de la |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad de Castilla La Mancha Ministerio de Economía y Competitividad (España) Artigas-Jerónimo, Sara [0000-0002-0068-5738] Villar, Margarita [0000-0003-4172-9079] Contreras, Marinela [0000-0002-1505-5624] Alberdi, Pilar [0000-0002-6999-9735] Cabezas-Cruz, Alejandro [0000-0002-8660-730X] Fuente, José de la [0000-0001-7383-9649] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Akirin Subolesin Art Yeast two-hybrid Evolution Music Vaccine Quantum vaccinomics |
| topic |
Akirin Subolesin Art Yeast two-hybrid Evolution Music Vaccine Quantum vaccinomics |
| description |
The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-κB pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR–protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-κB. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2–protein interactions in the regulation of the NF-κB pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020 2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/221383 |
| url |
http://hdl.handle.net/10261/221383 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-79892-P https://doi.org/10.3390/vaccines8010077 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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15,811543 |