Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry

8 páginas, 5 figuras.-- et al.

Detalles Bibliográficos
Autores: Azanza, María J., Pérez-Bruzón, R. N., Martínez-Ciriano, C., Pérez-Castejón, C., Vera-Gil, Alejandro, Moral, A. del
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/19485
Acceso en línea:http://hdl.handle.net/10261/19485
Access Level:acceso abierto
Palabra clave:Connexins
Immunocytochemistry
Helix aspersa
Synchronization
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spelling Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistryAzanza, María J.Pérez-Bruzón, R. N.Martínez-Ciriano, C.Pérez-Castejón, C.Vera-Gil, AlejandroMoral, A. delConnexinsImmunocytochemistryHelix aspersaSynchronization8 páginas, 5 figuras.-- et al.The aim of the present study was to examine the distribution of cells expressing connexin 26 (Cx26) in the suboesophageal visceral, left and right parietal and left and right pleural ganglia of the snail Helix aspersa by immunocytochemistry. Altogether we have found approximately 452 immunoreactive neurons which represent the 4.7% of the total neurons counted. The stained large neurons (measured diameter 55-140 µm) occurred mostly on the peripheral surface of the ganglia while the small immunostained cells (5-25 µm diameter) were observed in groups near the neuropil. The number of large neurons giving positive Cx26-like immunostaining was small in comparison with that for medium (30-50 µm diameter) and small sized cells. The expression of Cx26 was also observed in the processes of glia cells localized among neurons somata and in the neuropil showing that the antiserum recognized epitopes in both protoplasmic and fibrous glia cells of Helix aspersa. The neuropils of all ganglia showed fibers densely immunostained. While we have observed a good specificity for Cx26-antiserum in neurons, a lack of reaction for Cx43 antiserum was observed in neurons and glia cells. The reaction for enolase antiserum in neurons was light and non-specific and a lack of reaction in glia cells and processes for GFAP antiserum was observed. Although the percentage of positive neurons for Cx26 antiserum was low is suggested that in normal physiological conditions or under stimulation the expression of connexin could be increased. The observed results can be considered of interest in the interpretation of Helix aspersa elemental two neuron networks synchronizing activity, observed under applied extremely low frequency magnetic fields.This research has received financial support from “Fundación Humanismo y Ciencia” (Madrid) and from “Diputación General de Aragón” (Spain) under Project B43.Peer reviewedUniversidad de Murcia200920092007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501259768 bytesapplication/pdfhttp://hdl.handle.net/10261/19485reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.hh.um.es/Abstracts/Vol_22/22_5/22_5_497.htminfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/194852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
title Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
spellingShingle Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
Azanza, María J.
Connexins
Immunocytochemistry
Helix aspersa
Synchronization
title_short Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
title_full Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
title_fullStr Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
title_full_unstemmed Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
title_sort Localization of connexins in neurons and glia cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
dc.creator.none.fl_str_mv Azanza, María J.
Pérez-Bruzón, R. N.
Martínez-Ciriano, C.
Pérez-Castejón, C.
Vera-Gil, Alejandro
Moral, A. del
author Azanza, María J.
author_facet Azanza, María J.
Pérez-Bruzón, R. N.
Martínez-Ciriano, C.
Pérez-Castejón, C.
Vera-Gil, Alejandro
Moral, A. del
author_role author
author2 Pérez-Bruzón, R. N.
Martínez-Ciriano, C.
Pérez-Castejón, C.
Vera-Gil, Alejandro
Moral, A. del
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Connexins
Immunocytochemistry
Helix aspersa
Synchronization
topic Connexins
Immunocytochemistry
Helix aspersa
Synchronization
description 8 páginas, 5 figuras.-- et al.
publishDate 2007
dc.date.none.fl_str_mv 2007
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/19485
url http://hdl.handle.net/10261/19485
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.hh.um.es/Abstracts/Vol_22/22_5/22_5_497.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 259768 bytes
application/pdf
dc.publisher.none.fl_str_mv Universidad de Murcia
publisher.none.fl_str_mv Universidad de Murcia
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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