Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie

The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were...

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Detalhes bibliográficos
Autores: Betancor, Marina, Marín, Belén, Otero, Alicia, Hedman, Carlos, Romero, Antonio, Barrio, Tomás, Sevilla, Eloisa, Douet, Jean-Yves, Huor, Alvina, Badiola, Juan José, Andréoletti, Olivier, Bolea, Rosa
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Universidad de Zaragoza
Repositório:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:129915
Acesso em linha:http://zaguan.unizar.es/record/129915
Access Level:Acceso aberto
Descrição
Resumo:The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were intracerebrally challenged with atypical scrapie. Animals were euthanized without clinical signs of prion disease and tested negative for PrPSc accumulation by immunohistochemistry and western blotting. However, an emergence of BSE-like prion seeding activity was detected during in vitro propagation of brain samples from the inoculated animals. These findings suggest that atypical scrapie may represent a potential source of BSE infection in cattle.