Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development

Background: Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain transcription factors act as master regulators of developmental switches and organ specification. However, the mechanism...

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Autores: Pajoro, Alice, Madrigal, Pedro, Muiño, Jose M., Matus, José Tomás|||0000-0002-9196-1813, Jin, Jian, Mecchia, Martin A., Debernardi, Juan M., Palatnik, Javier F., Balazadeh, Salma, Arif, Muhammad, Ó'Maoiléidigh, Diarmuid S., Wellmer, Frank, Krajewski, Pawel, Riechmann, José Luis|||0000-0003-1108-1945, Angenent, Gerco C., Kaufmann, Kerstin
Formato: artículo
Fecha de publicación:2014
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:217719
Acesso em linha:https://ddd.uab.cat/record/217719
https://dx.doi.org/urn:doi:10.1186/gb-2014-15-3-r41
Access Level:acceso abierto
Palavra-chave:Arabidopsis
Arabidopsis Proteins
Chromatin
Chromatin Assembly and Disassembly
Flowers
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Homeodomain Proteins
MADS Domain Proteins
Protein Binding
Transcription Factors
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spelling Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower developmentPajoro, AliceMadrigal, PedroMuiño, Jose M.Matus, José Tomás|||0000-0002-9196-1813Jin, JianMecchia, Martin A.Debernardi, Juan M.Palatnik, Javier F.Balazadeh, SalmaArif, MuhammadÓ'Maoiléidigh, Diarmuid S.Wellmer, FrankKrajewski, PawelRiechmann, José Luis|||0000-0003-1108-1945Angenent, Gerco C.Kaufmann, KerstinArabidopsisArabidopsis ProteinsChromatinChromatin Assembly and DisassemblyFlowersGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantHomeodomain ProteinsMADS Domain ProteinsProtein BindingTranscription FactorsBackground: Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain transcription factors act as master regulators of developmental switches and organ specification. However, the mechanisms by which these factors dynamically regulate the expression of their target genes at different developmental stages are still poorly understood.Results: We characterized the relationship of chromatin accessibility, gene expression, and DNA binding of two MADS-domain proteins at different stages of Arabidopsis flower development. Dynamic changes in APETALA1 and SEPALLATA3 DNA binding correlated with changes in gene expression, and many of the target genes could be associated with the developmental stage in which they are transcriptionally controlled. We also observe dynamic changes in chromatin accessibility during flower development. Remarkably, DNA binding of APETALA1 and SEPALLATA3 is largely independent of the accessibility status of their binding regions and it can precede increases in DNA accessibility. These results suggest that APETALA1 and SEPALLATA3 may modulate chromatin accessibility, thereby facilitating access of other transcriptional regulators to their target genes.Conclusions: Our findings indicate that different homeotic factors regulate partly overlapping, yet also distinctive sets of target genes in a partly stage-specific fashion. By combining the information from DNA-binding and gene expression data, we are able to propose models of stage-specific regulatory interactions, thereby addressing dynamics of regulatory networks throughout flower development. Furthermore, MADS-domain TFs may regulate gene expression by alternative strategies, one of which is modulation of chromatin accessibility. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/217719https://dx.doi.org/urn:doi:10.1186/gb-2014-15-3-r41reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 237909Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BFU2011-22734open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2177192026-06-06T12:50:31Z
dc.title.none.fl_str_mv Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
title Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
spellingShingle Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
Pajoro, Alice
Arabidopsis
Arabidopsis Proteins
Chromatin
Chromatin Assembly and Disassembly
Flowers
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Homeodomain Proteins
MADS Domain Proteins
Protein Binding
Transcription Factors
title_short Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
title_full Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
title_fullStr Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
title_full_unstemmed Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
title_sort Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
dc.creator.none.fl_str_mv Pajoro, Alice
Madrigal, Pedro
Muiño, Jose M.
Matus, José Tomás|||0000-0002-9196-1813
Jin, Jian
Mecchia, Martin A.
Debernardi, Juan M.
Palatnik, Javier F.
Balazadeh, Salma
Arif, Muhammad
Ó'Maoiléidigh, Diarmuid S.
Wellmer, Frank
Krajewski, Pawel
Riechmann, José Luis|||0000-0003-1108-1945
Angenent, Gerco C.
Kaufmann, Kerstin
author Pajoro, Alice
author_facet Pajoro, Alice
Madrigal, Pedro
Muiño, Jose M.
Matus, José Tomás|||0000-0002-9196-1813
Jin, Jian
Mecchia, Martin A.
Debernardi, Juan M.
Palatnik, Javier F.
Balazadeh, Salma
Arif, Muhammad
Ó'Maoiléidigh, Diarmuid S.
Wellmer, Frank
Krajewski, Pawel
Riechmann, José Luis|||0000-0003-1108-1945
Angenent, Gerco C.
Kaufmann, Kerstin
author_role author
author2 Madrigal, Pedro
Muiño, Jose M.
Matus, José Tomás|||0000-0002-9196-1813
Jin, Jian
Mecchia, Martin A.
Debernardi, Juan M.
Palatnik, Javier F.
Balazadeh, Salma
Arif, Muhammad
Ó'Maoiléidigh, Diarmuid S.
Wellmer, Frank
Krajewski, Pawel
Riechmann, José Luis|||0000-0003-1108-1945
Angenent, Gerco C.
Kaufmann, Kerstin
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
Arabidopsis Proteins
Chromatin
Chromatin Assembly and Disassembly
Flowers
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Homeodomain Proteins
MADS Domain Proteins
Protein Binding
Transcription Factors
topic Arabidopsis
Arabidopsis Proteins
Chromatin
Chromatin Assembly and Disassembly
Flowers
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Homeodomain Proteins
MADS Domain Proteins
Protein Binding
Transcription Factors
description Background: Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain transcription factors act as master regulators of developmental switches and organ specification. However, the mechanisms by which these factors dynamically regulate the expression of their target genes at different developmental stages are still poorly understood.Results: We characterized the relationship of chromatin accessibility, gene expression, and DNA binding of two MADS-domain proteins at different stages of Arabidopsis flower development. Dynamic changes in APETALA1 and SEPALLATA3 DNA binding correlated with changes in gene expression, and many of the target genes could be associated with the developmental stage in which they are transcriptionally controlled. We also observe dynamic changes in chromatin accessibility during flower development. Remarkably, DNA binding of APETALA1 and SEPALLATA3 is largely independent of the accessibility status of their binding regions and it can precede increases in DNA accessibility. These results suggest that APETALA1 and SEPALLATA3 may modulate chromatin accessibility, thereby facilitating access of other transcriptional regulators to their target genes.Conclusions: Our findings indicate that different homeotic factors regulate partly overlapping, yet also distinctive sets of target genes in a partly stage-specific fashion. By combining the information from DNA-binding and gene expression data, we are able to propose models of stage-specific regulatory interactions, thereby addressing dynamics of regulatory networks throughout flower development. Furthermore, MADS-domain TFs may regulate gene expression by alternative strategies, one of which is modulation of chromatin accessibility.
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/217719
https://dx.doi.org/urn:doi:10.1186/gb-2014-15-3-r41
url https://ddd.uab.cat/record/217719
https://dx.doi.org/urn:doi:10.1186/gb-2014-15-3-r41
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 237909
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 BFU2011-22734
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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