Extending calibration-free force measurements to optically-trapped rod-shaped samples

Optical trapping has become an optimal choice for biological research at the microscale due to its non-invasive performance and accessibility for quantitative studies, especially on the forces involved in biological processes. However, reliable force measurements depend on the calibration of the opt...

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Detalles Bibliográficos
Autores: Català, Frederic, Marsà, Ferran, Montes Usategui, Mario, Farré Flaquer, Arnau, Martín Badosa, Estela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/120437
Acceso en línea:https://hdl.handle.net/2445/120437
Access Level:acceso abierto
Palabra clave:Fotònica
Òptica física
Photonics
Physical optics
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spelling Extending calibration-free force measurements to optically-trapped rod-shaped samplesCatalà, FredericMarsà, FerranMontes Usategui, MarioFarré Flaquer, ArnauMartín Badosa, EstelaFotònicaÒptica físicaPhotonicsPhysical opticsOptical trapping has become an optimal choice for biological research at the microscale due to its non-invasive performance and accessibility for quantitative studies, especially on the forces involved in biological processes. However, reliable force measurements depend on the calibration of the optical traps, which is different for each experiment and hence requires high control of the local variables, especially of the trapped object geometry. Many biological samples have an elongated, rod-like shape, such as chromosomes, intracellular organelles (e.g., peroxisomes), membrane tubules, certain microalgae, and a wide variety of bacteria and parasites. This type of samples often requires several optical traps to stabilize and orient them in the correct spatial direction, making it more difficult to determine the total force applied. Here, we manipulate glass microcylinders with holographic optical tweezers and show the accurate measurement of drag forces by calibration-free direct detection of beam momentum. The agreement between our results and slender-body hydrodynamic theoretical calculations indicates potential for this force-sensing method in studying protracted, rod-shaped specimens.Nature Publishing Group2018201820172018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/120437Articles publicats en revistes (Física Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/srep42960Scientific Reports, 2017, vol. 7, num. 42960https://doi.org/10.1038/srep42960cc-by (c) Català, Frederic et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1204372026-05-29T05:05:01Z
dc.title.none.fl_str_mv Extending calibration-free force measurements to optically-trapped rod-shaped samples
title Extending calibration-free force measurements to optically-trapped rod-shaped samples
spellingShingle Extending calibration-free force measurements to optically-trapped rod-shaped samples
Català, Frederic
Fotònica
Òptica física
Photonics
Physical optics
title_short Extending calibration-free force measurements to optically-trapped rod-shaped samples
title_full Extending calibration-free force measurements to optically-trapped rod-shaped samples
title_fullStr Extending calibration-free force measurements to optically-trapped rod-shaped samples
title_full_unstemmed Extending calibration-free force measurements to optically-trapped rod-shaped samples
title_sort Extending calibration-free force measurements to optically-trapped rod-shaped samples
dc.creator.none.fl_str_mv Català, Frederic
Marsà, Ferran
Montes Usategui, Mario
Farré Flaquer, Arnau
Martín Badosa, Estela
author Català, Frederic
author_facet Català, Frederic
Marsà, Ferran
Montes Usategui, Mario
Farré Flaquer, Arnau
Martín Badosa, Estela
author_role author
author2 Marsà, Ferran
Montes Usategui, Mario
Farré Flaquer, Arnau
Martín Badosa, Estela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fotònica
Òptica física
Photonics
Physical optics
topic Fotònica
Òptica física
Photonics
Physical optics
description Optical trapping has become an optimal choice for biological research at the microscale due to its non-invasive performance and accessibility for quantitative studies, especially on the forces involved in biological processes. However, reliable force measurements depend on the calibration of the optical traps, which is different for each experiment and hence requires high control of the local variables, especially of the trapped object geometry. Many biological samples have an elongated, rod-like shape, such as chromosomes, intracellular organelles (e.g., peroxisomes), membrane tubules, certain microalgae, and a wide variety of bacteria and parasites. This type of samples often requires several optical traps to stabilize and orient them in the correct spatial direction, making it more difficult to determine the total force applied. Here, we manipulate glass microcylinders with holographic optical tweezers and show the accurate measurement of drag forces by calibration-free direct detection of beam momentum. The agreement between our results and slender-body hydrodynamic theoretical calculations indicates potential for this force-sensing method in studying protracted, rod-shaped specimens.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/120437
url https://hdl.handle.net/2445/120437
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/srep42960
Scientific Reports, 2017, vol. 7, num. 42960
https://doi.org/10.1038/srep42960
dc.rights.none.fl_str_mv cc-by (c) Català, Frederic et al., 2017
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Català, Frederic et al., 2017
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Física Aplicada)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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