Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract

A TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 c...

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Autores: García-Villalba, Rocío, Vissenaekens, Hanne, Pitart, Judit, Romo Vaquero, María, Espín de Gea, Juan Carlos, Grootaert, Charlotte, Selma, María Victoria, Raes, Katleen, Smagghe, Guy, Possemiers, Sam, Van Camp, John, Tomás Barberán, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/166564
Acceso en línea:http://hdl.handle.net/10261/166564
Access Level:acceso abierto
Palabra clave:Phenotypes
Urolithin
Intestinal cells
Gut microbiota
Ellagic acid
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spelling Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tractGarcía-Villalba, RocíoVissenaekens, HannePitart, JuditRomo Vaquero, MaríaEspín de Gea, Juan CarlosGrootaert, CharlotteSelma, María VictoriaRaes, KatleenSmagghe, GuyPossemiers, SamVan Camp, JohnTomás Barberán, FranciscoPhenotypesUrolithinIntestinal cellsGut microbiotaEllagic acidA TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.This work was funded by the Projects BACCHUS (FP7-KBBE2012-6-single stage, European Commission Grant Agreement 312090), CICYT AGL2011-22447, AGL201564124-R (MINECO, Spain), 201370E068 (CSIC, Spain), and Fundacion Seneca (19900/GERM/15). H.V. is a Ph.D. Fellowship Strategic Basic Researcher of the Research Foundation-Flanders (FWO). We thank the Special Research Fund of Ghent University (BOF 01B04212) for the funding of the TEER equipment.We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).Peer ReviewedAmerican Chemical SocietyMinisterio de Economía y Competitividad (España)Fundación SénecaResearch Foundation - FlandersConsejo Superior de Investigaciones Científicas (España)European CommissionComisión Interministerial de Ciencia y Tecnología, CICYT (España)Ghent UniversityCSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2018201820172018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/166564reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64124-Rinfo:eu-repo/grantAgreement/EC/FP7/312090https://doi.org/10.1021/acs.jafc.7b02049Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1665642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
title Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
spellingShingle Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
García-Villalba, Rocío
Phenotypes
Urolithin
Intestinal cells
Gut microbiota
Ellagic acid
title_short Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
title_full Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
title_fullStr Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
title_full_unstemmed Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
title_sort Gastrointestinal simulation model TWIN-SHIME shows differences between human urolithin-metabotypes in gut microbiota composition, pomegranate polyphenol metabolism, and transport along the intestinal tract
dc.creator.none.fl_str_mv García-Villalba, Rocío
Vissenaekens, Hanne
Pitart, Judit
Romo Vaquero, María
Espín de Gea, Juan Carlos
Grootaert, Charlotte
Selma, María Victoria
Raes, Katleen
Smagghe, Guy
Possemiers, Sam
Van Camp, John
Tomás Barberán, Francisco
author García-Villalba, Rocío
author_facet García-Villalba, Rocío
Vissenaekens, Hanne
Pitart, Judit
Romo Vaquero, María
Espín de Gea, Juan Carlos
Grootaert, Charlotte
Selma, María Victoria
Raes, Katleen
Smagghe, Guy
Possemiers, Sam
Van Camp, John
Tomás Barberán, Francisco
author_role author
author2 Vissenaekens, Hanne
Pitart, Judit
Romo Vaquero, María
Espín de Gea, Juan Carlos
Grootaert, Charlotte
Selma, María Victoria
Raes, Katleen
Smagghe, Guy
Possemiers, Sam
Van Camp, John
Tomás Barberán, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Séneca
Research Foundation - Flanders
Consejo Superior de Investigaciones Científicas (España)
European Commission
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Ghent University
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Phenotypes
Urolithin
Intestinal cells
Gut microbiota
Ellagic acid
topic Phenotypes
Urolithin
Intestinal cells
Gut microbiota
Ellagic acid
description A TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/166564
url http://hdl.handle.net/10261/166564
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64124-R
info:eu-repo/grantAgreement/EC/FP7/312090
https://doi.org/10.1021/acs.jafc.7b02049
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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