Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure

We investigated the effects of bottle enclosure on autotrophic and heterotrophic picoplankton in North and South subtropical Atlantic oligotrophic waters, where the biomass and metabolism of the microbial community are dominated by the picoplankton size class. We measured changes in both autotrophic...

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Authors: Calvo-Díaz, Alejandra, Díaz-Pérez, Laura, Suárez, L.A., Morán, Xosé Anxelu G., Teira, Eva, Marañón, E.
Format: article
Publication Date:2011
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/319527
Online Access:http://hdl.handle.net/10261/319527
Access Level:Open access
Keyword:Centro Oceanográfico de Gijón
Medio Marino
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repository_id_str
spelling Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosureCalvo-Díaz, AlejandraDíaz-Pérez, LauraSuárez, L.A.Morán, Xosé Anxelu G.Teira, EvaMarañón, E.Centro Oceanográfico de GijónMedio MarinoWe investigated the effects of bottle enclosure on autotrophic and heterotrophic picoplankton in North and South subtropical Atlantic oligotrophic waters, where the biomass and metabolism of the microbial community are dominated by the picoplankton size class. We measured changes in both autotrophic (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic picoplankton biomass during three time series experiments and in 16 endpoint experiments over 24 h in light and dark treatments. Our results showed a divergent effect of bottle incubation on the autotrophic and heterotrophic components of the picoplankton community. The biomass of picophytoplankton showed, on average, a >50% decrease, mostly affecting the picoeukaryotes and, to a lesser extent, Prochlorococcus. In contrast, the biomass of heterotrophic bacteria remained constant or increased during the incubations. We also sampled 10 stations during a Lagrangian study in the North Atlantic subtropical gyre, which enabled us to compare the observed changes in the auto- to heterotrophic picoplankton biomass ratio (AB:HB ratio) inside the incubation bottles with those taking place in situ. While the AB:HB ratio in situ remained fairly constant during the Lagrangian study, it decreased significantly during the 24 h of incubation experiments. Thus, the rapid biomass changes observed in the incubations are artifacts resulting from bottle confinement and do not take place in natural conditions. Our results suggest that short (<1 day) bottle incubations in oligotrophic waters may lead to biased estimates of the microbial metabolic balance by underestimating primary production and/or overestimating bacterial respiration.Sí202320232011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/319527reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Gijóninfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3195272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
title Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
spellingShingle Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
Calvo-Díaz, Alejandra
Centro Oceanográfico de Gijón
Medio Marino
title_short Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
title_full Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
title_fullStr Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
title_full_unstemmed Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
title_sort Decrease in the autotrophic-heterotrophic biomass ratio of picoplankton in oligotrophic marine waters due to bottle enclosure
dc.creator.none.fl_str_mv Calvo-Díaz, Alejandra
Díaz-Pérez, Laura
Suárez, L.A.
Morán, Xosé Anxelu G.
Teira, Eva
Marañón, E.
author Calvo-Díaz, Alejandra
author_facet Calvo-Díaz, Alejandra
Díaz-Pérez, Laura
Suárez, L.A.
Morán, Xosé Anxelu G.
Teira, Eva
Marañón, E.
author_role author
author2 Díaz-Pérez, Laura
Suárez, L.A.
Morán, Xosé Anxelu G.
Teira, Eva
Marañón, E.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Centro Oceanográfico de Gijón
Medio Marino
topic Centro Oceanográfico de Gijón
Medio Marino
description We investigated the effects of bottle enclosure on autotrophic and heterotrophic picoplankton in North and South subtropical Atlantic oligotrophic waters, where the biomass and metabolism of the microbial community are dominated by the picoplankton size class. We measured changes in both autotrophic (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic picoplankton biomass during three time series experiments and in 16 endpoint experiments over 24 h in light and dark treatments. Our results showed a divergent effect of bottle incubation on the autotrophic and heterotrophic components of the picoplankton community. The biomass of picophytoplankton showed, on average, a >50% decrease, mostly affecting the picoeukaryotes and, to a lesser extent, Prochlorococcus. In contrast, the biomass of heterotrophic bacteria remained constant or increased during the incubations. We also sampled 10 stations during a Lagrangian study in the North Atlantic subtropical gyre, which enabled us to compare the observed changes in the auto- to heterotrophic picoplankton biomass ratio (AB:HB ratio) inside the incubation bottles with those taking place in situ. While the AB:HB ratio in situ remained fairly constant during the Lagrangian study, it decreased significantly during the 24 h of incubation experiments. Thus, the rapid biomass changes observed in the incubations are artifacts resulting from bottle confinement and do not take place in natural conditions. Our results suggest that short (<1 day) bottle incubations in oligotrophic waters may lead to biased estimates of the microbial metabolic balance by underestimating primary production and/or overestimating bacterial respiration.
publishDate 2011
dc.date.none.fl_str_mv 2011
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/319527
url http://hdl.handle.net/10261/319527
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Gijón
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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