Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)

Peroxidases (EC 1.11.1.7) are a large group of enzymes widely distributed in the plant kingdom. The present work describes a study on the isolation, purification and some features of a novel peroxidase from white Spanish broom (Cytisus multiflorus), a tree legume very abundant in the northern half o...

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Detalles Bibliográficos
Autores: Pérez Galende, Patricia, García de María, Cándido, Arellano, Juan B., Roig, Manuel G., Shnyrov, Valery L.
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/144106
Acceso en línea:http://hdl.handle.net/10261/144106
Access Level:acceso abierto
Palabra clave:peroxidase
protein purification
Cytisus multiflorus
Chromatography
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spelling Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)Pérez Galende, PatriciaGarcía de María, CándidoArellano, Juan B.Roig, Manuel G.Shnyrov, Valery L.peroxidaseprotein purificationCytisus multiflorusChromatographyPeroxidases (EC 1.11.1.7) are a large group of enzymes widely distributed in the plant kingdom. The present work describes a study on the isolation, purification and some features of a novel peroxidase from white Spanish broom (Cytisus multiflorus), a tree legume very abundant in the northern half of Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from Cytisus multiflorus (CMP) was found to have a molecular weight of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of 3.3. Substrate specificity and the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.This work was partially supported by project SA052A10-2, funded by Consejería de Educación de la Junta de Castilla y León (Spain).Peer reviewedJSciMed CentralConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/144106reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://www.jscimedcentral.com/PlantBiology/plantbiology-4-1052.pdfSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1441062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
title Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
spellingShingle Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
Pérez Galende, Patricia
peroxidase
protein purification
Cytisus multiflorus
Chromatography
title_short Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
title_full Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
title_fullStr Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
title_full_unstemmed Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
title_sort Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)
dc.creator.none.fl_str_mv Pérez Galende, Patricia
García de María, Cándido
Arellano, Juan B.
Roig, Manuel G.
Shnyrov, Valery L.
author Pérez Galende, Patricia
author_facet Pérez Galende, Patricia
García de María, Cándido
Arellano, Juan B.
Roig, Manuel G.
Shnyrov, Valery L.
author_role author
author2 García de María, Cándido
Arellano, Juan B.
Roig, Manuel G.
Shnyrov, Valery L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv peroxidase
protein purification
Cytisus multiflorus
Chromatography
topic peroxidase
protein purification
Cytisus multiflorus
Chromatography
description Peroxidases (EC 1.11.1.7) are a large group of enzymes widely distributed in the plant kingdom. The present work describes a study on the isolation, purification and some features of a novel peroxidase from white Spanish broom (Cytisus multiflorus), a tree legume very abundant in the northern half of Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from Cytisus multiflorus (CMP) was found to have a molecular weight of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of 3.3. Substrate specificity and the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/144106
url http://hdl.handle.net/10261/144106
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.jscimedcentral.com/PlantBiology/plantbiology-4-1052.pdf
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv JSciMed Central
publisher.none.fl_str_mv JSciMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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