The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis
© 2021 The Authors..
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/260300 |
| Acceso en línea: | http://hdl.handle.net/10261/260300 |
| Access Level: | acceso abierto |
| Palabra clave: | Drosophila Centrosomes Polo kinase APC/C Germline Fertility |
| id |
ES_24a2e65feea70d61aeed3bfca7fcb6d7 |
|---|---|
| oai_identifier_str |
oai:digital.csic.es:10261/260300 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesisLeah Braun, AlexisMeghini, F.Villa-Fombuena, GemaGuermont, M.Fernandez-Martinez, ElisaQian, ZhangMartín-Bermudo, María D.González-Reyes, AcaimoMoore Glover, DavidKimata, Y.DrosophilaCentrosomesPolo kinaseAPC/CGermlineFertility© 2021 The Authors..A feature of metazoan reproduction is the elimination of maternal centrosomes from the oocyte. In animals that form syncytial cysts during oogenesis, including Drosophila and human, all centrosomes within the cyst migrate to the oocyte where they are subsequently degenerated. The importance and the underlying mechanism of this event remain unclear. Here, we show that, during early Drosophila oogenesis, control of the Anaphase Promoting Complex/Cyclosome (APC/C), the ubiquitin ligase complex essential for cell cycle control, ensures proper transport of centrosomes into the oocyte through the regulation of Polo/Plk1 kinase, a critical regulator of the integrity and activity of the centrosome. We show that novel mutations in the APC/C-specific E2, Vihar/Ube2c, that affect its inhibitory regulation on APC/C cause precocious Polo degradation and impedes centrosome transport, through destabilization of centrosomes. The failure of centrosome migration correlates with weakened microtubule polarization in the cyst and allows ectopic microtubule nucleation in nurse cells, leading to the loss of oocyte identity. These results suggest a role for centrosome migration in oocyte fate maintenance through the concentration and confinement of microtubule nucleation activity into the oocyte. Considering the conserved roles of APC/C and Polo throughout the animal kingdom, our findings may be translated into other animals.This work was supported by the Cancer Research UK Career Development Fellowship (CRUK-A12874) and the ShanghaiTech University start-up grant (2018F0202-000-06) to Y.K. and the Wellcome Trust Institutional Strategic Support Fund (ISSF) to A.L.B. and Cancer Research UK (CRUK-RG78567).Royal Society (Great Britain)Cancer Research UKShanghaiTech UniversityWellcome Sanger InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/260300reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1098/rsob.200371Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2603002026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| title |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| spellingShingle |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis Leah Braun, Alexis Drosophila Centrosomes Polo kinase APC/C Germline Fertility |
| title_short |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| title_full |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| title_fullStr |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| title_full_unstemmed |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| title_sort |
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis |
| dc.creator.none.fl_str_mv |
Leah Braun, Alexis Meghini, F. Villa-Fombuena, Gema Guermont, M. Fernandez-Martinez, Elisa Qian, Zhang Martín-Bermudo, María D. González-Reyes, Acaimo Moore Glover, David Kimata, Y. |
| author |
Leah Braun, Alexis |
| author_facet |
Leah Braun, Alexis Meghini, F. Villa-Fombuena, Gema Guermont, M. Fernandez-Martinez, Elisa Qian, Zhang Martín-Bermudo, María D. González-Reyes, Acaimo Moore Glover, David Kimata, Y. |
| author_role |
author |
| author2 |
Meghini, F. Villa-Fombuena, Gema Guermont, M. Fernandez-Martinez, Elisa Qian, Zhang Martín-Bermudo, María D. González-Reyes, Acaimo Moore Glover, David Kimata, Y. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Cancer Research UK ShanghaiTech University Wellcome Sanger Institute Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Drosophila Centrosomes Polo kinase APC/C Germline Fertility |
| topic |
Drosophila Centrosomes Polo kinase APC/C Germline Fertility |
| description |
© 2021 The Authors.. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/260300 |
| url |
http://hdl.handle.net/10261/260300 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://dx.doi.org/10.1098/rsob.200371 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Royal Society (Great Britain) |
| publisher.none.fl_str_mv |
Royal Society (Great Britain) |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869404710870450176 |
| score |
15.81155 |