MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION

Background:Ventilatory strategy and specifically positive end-expiratory pressure (PEEP) can modulate the inflammatory response and pulmonary-to-systemic translocation of lipopolysaccharide (LPS). Both inflammation and ventilatory pattern may modify brain activation, possibly worsening the patient&#...

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Autores: Elisa Quilez, Maria, Rodriguez-Gonzalez, Raquel, Turon, MARC, Fernandez-Gonzalo, Sol, Villar, Jesus, Kacmarek, Robert M., Nieves Gomez, Ma, Carles Oliva, Joan, Blanch, Lluis, Lopez-Aguilar, Josefina
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Institut d'Investigació i Innovació Parc Taulí (I3PT)
Repositorio:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
OAI Identifier:oai:i3pt.fundanetsuite.com:p4541
Acesso em linha:https://i3pt.portalinvestigacion.com/publicaciones/4541
Access Level:acceso abierto
Palavra-chave:Experimental
inflammation
mechanical ventilation
neuronal activation
PEEP
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spelling MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATIONElisa Quilez, MariaRodriguez-Gonzalez, RaquelTuron, MARCFernandez-Gonzalo, SolVillar, JesusKacmarek, Robert M.Nieves Gomez, MaCarles Oliva, JoanBlanch, LluisLopez-Aguilar, JosefinaExperimentalinflammationmechanical ventilationneuronal activationPEEPBackground:Ventilatory strategy and specifically positive end-expiratory pressure (PEEP) can modulate the inflammatory response and pulmonary-to-systemic translocation of lipopolysaccharide (LPS). Both inflammation and ventilatory pattern may modify brain activation, possibly worsening the patient's outcome and resulting in cognitive sequelae.Methods:We prospectively studied Sprague-Dawley rats randomly assigned to undergo 3h mechanical ventilation with 7mL/kg tidal ventilation and either 2cmH(2)O or 7cmH(2)O PEEP after intratracheal instillation of LPS or saline. Healthy nonventilated rats served as baseline. We analyzed lung mechanics, gas exchange, lung and plasma cytokine levels, lung apoptotic cells, and lung neutrophil infiltration. To evaluate brain neuronal activation, we counted c-Fos immunopositive cells in the retrosplenial cortex (RS), thalamus, supraoptic nucleus (SON), nucleus of the solitary tract (NTS), paraventricular nucleus (PVN), and central amygdala (CeA).Results:LPS increased lung neutrophilic infiltration, lung and systemic MCP-1 levels, and neuronal activation in the CeA and NTS. LPS-instilled rats receiving 7cmH(2)O PEEP had less lung and systemic inflammation and more c-Fos-immunopositive cells in the RS, SON, and thalamus than those receiving 2cmH(2)O PEEP. Applying 7cmH(2)O PEEP increased neuronal activation in the CeA and NTS in saline-instilled rats, but not in LPS-instilled rats.Conclusions:Moderate PEEP prevented lung and systemic inflammation secondary to intratracheal LPS instillation. PEEP also modified the neuronal activation pattern in the RS, SON, and thalamus. The relevance of these differential brain c-Fos expression patterns in neurocognitive outcomes should be explored.LIPPINCOTT WILLIAMS & WILKINS2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://i3pt.portalinvestigacion.com/publicaciones/4541SHOCKISSN: 10732322ISSNe: 15400514reponame:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulíinstname:Institut d'Investigació i Innovació Parc Taulí (I3PT)Inglésinfo:eu-repo/semantics/openAccessoai:i3pt.fundanetsuite.com:p45412026-06-21T15:30:37Z
dc.title.none.fl_str_mv MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
title MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
spellingShingle MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
Elisa Quilez, Maria
Experimental
inflammation
mechanical ventilation
neuronal activation
PEEP
title_short MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
title_full MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
title_fullStr MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
title_full_unstemmed MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
title_sort MODERATE PEEP AFTER TRACHEAL LIPOPOLYSACCHARIDE INSTILLATION PREVENTS INFLAMMATION AND MODIFIES THE PATTERN OF BRAIN NEURONAL ACTIVATION
dc.creator.none.fl_str_mv Elisa Quilez, Maria
Rodriguez-Gonzalez, Raquel
Turon, MARC
Fernandez-Gonzalo, Sol
Villar, Jesus
Kacmarek, Robert M.
Nieves Gomez, Ma
Carles Oliva, Joan
Blanch, Lluis
Lopez-Aguilar, Josefina
author Elisa Quilez, Maria
author_facet Elisa Quilez, Maria
Rodriguez-Gonzalez, Raquel
Turon, MARC
Fernandez-Gonzalo, Sol
Villar, Jesus
Kacmarek, Robert M.
Nieves Gomez, Ma
Carles Oliva, Joan
Blanch, Lluis
Lopez-Aguilar, Josefina
author_role author
author2 Rodriguez-Gonzalez, Raquel
Turon, MARC
Fernandez-Gonzalo, Sol
Villar, Jesus
Kacmarek, Robert M.
Nieves Gomez, Ma
Carles Oliva, Joan
Blanch, Lluis
Lopez-Aguilar, Josefina
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Experimental
inflammation
mechanical ventilation
neuronal activation
PEEP
topic Experimental
inflammation
mechanical ventilation
neuronal activation
PEEP
description Background:Ventilatory strategy and specifically positive end-expiratory pressure (PEEP) can modulate the inflammatory response and pulmonary-to-systemic translocation of lipopolysaccharide (LPS). Both inflammation and ventilatory pattern may modify brain activation, possibly worsening the patient's outcome and resulting in cognitive sequelae.Methods:We prospectively studied Sprague-Dawley rats randomly assigned to undergo 3h mechanical ventilation with 7mL/kg tidal ventilation and either 2cmH(2)O or 7cmH(2)O PEEP after intratracheal instillation of LPS or saline. Healthy nonventilated rats served as baseline. We analyzed lung mechanics, gas exchange, lung and plasma cytokine levels, lung apoptotic cells, and lung neutrophil infiltration. To evaluate brain neuronal activation, we counted c-Fos immunopositive cells in the retrosplenial cortex (RS), thalamus, supraoptic nucleus (SON), nucleus of the solitary tract (NTS), paraventricular nucleus (PVN), and central amygdala (CeA).Results:LPS increased lung neutrophilic infiltration, lung and systemic MCP-1 levels, and neuronal activation in the CeA and NTS. LPS-instilled rats receiving 7cmH(2)O PEEP had less lung and systemic inflammation and more c-Fos-immunopositive cells in the RS, SON, and thalamus than those receiving 2cmH(2)O PEEP. Applying 7cmH(2)O PEEP increased neuronal activation in the CeA and NTS in saline-instilled rats, but not in LPS-instilled rats.Conclusions:Moderate PEEP prevented lung and systemic inflammation secondary to intratracheal LPS instillation. PEEP also modified the neuronal activation pattern in the RS, SON, and thalamus. The relevance of these differential brain c-Fos expression patterns in neurocognitive outcomes should be explored.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://i3pt.portalinvestigacion.com/publicaciones/4541
url https://i3pt.portalinvestigacion.com/publicaciones/4541
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv LIPPINCOTT WILLIAMS & WILKINS
publisher.none.fl_str_mv LIPPINCOTT WILLIAMS & WILKINS
dc.source.none.fl_str_mv SHOCK
ISSN: 10732322
ISSNe: 15400514
reponame:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
instname:Institut d'Investigació i Innovació Parc Taulí (I3PT)
instname_str Institut d'Investigació i Innovació Parc Taulí (I3PT)
reponame_str r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
collection r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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