RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro

9 pages, 6 figures.-- PMID: 17005683 [PubMed].-- PMCID: PMC1617295.-- Supporting information available at: http://jvi.asm.org/cgi/content/full/80/20/10055/DC1

Detalles Bibliográficos
Autores: Valli, Adrián, Martín-Hernández, Ana Montserrat, López Moya, Juan José, García, Juan Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/28896
Acceso en línea:http://hdl.handle.net/10261/28896
Access Level:acceso abierto
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spelling RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCProValli, AdriánMartín-Hernández, Ana MontserratLópez Moya, Juan JoséGarcía, Juan Antonio9 pages, 6 figures.-- PMID: 17005683 [PubMed].-- PMCID: PMC1617295.-- Supporting information available at: http://jvi.asm.org/cgi/content/full/80/20/10055/DC1The P1 protein of viruses of the family Potyviridae is a serine proteinase, which is highly variable in length and sequence, and its role in the virus infection cycle is not clear. One of the proposed activities of P1 is to assist HCPro, the product that viruses of the genus Potyvirus use to counteract antiviral defense mediated by RNA silencing. Indeed, an HCPro-coding region is present in all the genomes of members of the genera Potyvirus, Rymovirus, and Tritimovirus that have been sequenced. However, it was recently reported that a sequence coding for HCPro is lacking in the genome of Cucumber vein yellowing virus (CVYV), a member of the genus Ipomovirus, the fourth monopartite genus of the family. In this study, we provide further evidence that P1 enhances the activity of HCPro in members of the genus Potyvirus and show that it is duplicated in the ipomovirus CVYV. The two CVYV P1 copies are arranged in tandem, and the second copy (P1b) has RNA silencing suppression activity. CVYV P1b suppressed RNA silencing induced either by sense green fluorescent protein (GFP) mRNA or by a GFP inverted repeat RNA, indicating that CVYV P1b acts downstream of the formation of double-stranded RNA. CVYV P1b also suppressed local silencing in agroinfiltrated patches of transgenic Nicotiana benthamiana line 16c and delayed its propagation to the neighboring cells. However, neither the short-distance nor long-distance systemic spread of silencing of the GFP transgene was completely blocked by CVYV P1b. CVYV P1b and P1-HCPro from the potyvirus Plum pox virus showed very similar behaviors in all the assays carried out, suggesting that evolution has found a way to counteract RNA silencing by similar mechanisms using very different proteins in viruses of the same family.This work was supported by grant BIO2004-02687 from the Spanish MEC and grants QLG2-CT-2002-01673 and SP22-CT-2004 from the European Union to J.A.G. Work in CSIC-IRTA laboratories was performed within the framework of the Centre de Referència en Biotecnologia (CeRBa) of the Generalitat de Catalunya, and it was supported by grant AGL2004-00704 from the Spanish MEC. A.V. was a recipient of an I3P fellowship from CSIC-Fondo Social Europeo, and A.M.M.-H. received a Ramón y Cajal contract from the Spanish MEC.Peer reviewedAmerican Society for MicrobiologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201020102006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/28896reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/JVI.00985-06Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/288962026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
title RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
spellingShingle RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
Valli, Adrián
title_short RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
title_full RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
title_fullStr RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
title_full_unstemmed RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
title_sort RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro
dc.creator.none.fl_str_mv Valli, Adrián
Martín-Hernández, Ana Montserrat
López Moya, Juan José
García, Juan Antonio
author Valli, Adrián
author_facet Valli, Adrián
Martín-Hernández, Ana Montserrat
López Moya, Juan José
García, Juan Antonio
author_role author
author2 Martín-Hernández, Ana Montserrat
López Moya, Juan José
García, Juan Antonio
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 9 pages, 6 figures.-- PMID: 17005683 [PubMed].-- PMCID: PMC1617295.-- Supporting information available at: http://jvi.asm.org/cgi/content/full/80/20/10055/DC1
publishDate 2006
dc.date.none.fl_str_mv 2006
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/28896
url http://hdl.handle.net/10261/28896
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/JVI.00985-06
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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