Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot

Self-incompatibility (SI) is one of the most efficient mechanisms to promote out-crossing in plants. However, SI could be a problem for fruit production. An example is apricot (Prunus armeniaca), in which, as in other species of the Rosaceae, SI is determined by an S-RNase-based-Gametophytic Self-In...

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Autores: Herrera, S., Rodrigo, J., Hormaza, J.I., Lora, J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:77026
Acceso en línea:http://zaguan.unizar.es/record/77026
Access Level:acceso abierto
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spelling Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricotHerrera, S.Rodrigo, J.Hormaza, J.I.Lora, J.Self-incompatibility (SI) is one of the most efficient mechanisms to promote out-crossing in plants. However, SI could be a problem for fruit production. An example is apricot (Prunus armeniaca), in which, as in other species of the Rosaceae, SI is determined by an S-RNase-based-Gametophytic Self-Incompatibility (GSI) system. Incompatibility relationships between cultivars can be established by an S-allele genotyping PCR strategy. Until recently, most of the traditional European apricot cultivars were self-compatible but several breeding programs have introduced an increasing number of new cultivars whose pollination requirements are unknown. To fill this gap, we have identified the S-allele of 44 apricot genotypes, of which 43 are reported here for the first time. The identification of S-c in 15 genotypes suggests that those cultivars are self-compatible. In five genotypes, self-(in)compatibility was established by the observation of pollen tube growth in self-pollinated flowers, since PCR analysis could not allowed distinguishing between the S-c and S-8 alleles. Self-incompatible genotypes were assigned to their corresponding self-incompatibility groups. The knowledge of incompatibility relationships between apricot cultivars can be a highly valuable tool for the development of future breeding programs by selecting the appropriate parents and for efficient orchard design by planting self-compatible and inter-compatible cultivars.2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/77026reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/DGA/A12-17Rinfo:eu-repo/grantAgreement/ES/INIA/RFP2015-00015-00info:eu-repo/grantAgreement/ES/INIA/RTA2014-00085-00info:eu-repo/grantAgreement/ES/INIA/RTA2017-00003-00info:eu-repo/grantAgreement/ES/MICINN/AGL2015-74071-JINinfo:eu-repo/grantAgreement/ES/MICINN/AGL2016-77267-Rinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:770262026-05-29T13:59:51Z
dc.title.none.fl_str_mv Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
title Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
spellingShingle Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
Herrera, S.
title_short Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
title_full Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
title_fullStr Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
title_full_unstemmed Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
title_sort Identification of self-incompatibility alleles by specific PCR analysis and S-RNase sequencing in apricot
dc.creator.none.fl_str_mv Herrera, S.
Rodrigo, J.
Hormaza, J.I.
Lora, J.
author Herrera, S.
author_facet Herrera, S.
Rodrigo, J.
Hormaza, J.I.
Lora, J.
author_role author
author2 Rodrigo, J.
Hormaza, J.I.
Lora, J.
author2_role author
author
author
description Self-incompatibility (SI) is one of the most efficient mechanisms to promote out-crossing in plants. However, SI could be a problem for fruit production. An example is apricot (Prunus armeniaca), in which, as in other species of the Rosaceae, SI is determined by an S-RNase-based-Gametophytic Self-Incompatibility (GSI) system. Incompatibility relationships between cultivars can be established by an S-allele genotyping PCR strategy. Until recently, most of the traditional European apricot cultivars were self-compatible but several breeding programs have introduced an increasing number of new cultivars whose pollination requirements are unknown. To fill this gap, we have identified the S-allele of 44 apricot genotypes, of which 43 are reported here for the first time. The identification of S-c in 15 genotypes suggests that those cultivars are self-compatible. In five genotypes, self-(in)compatibility was established by the observation of pollen tube growth in self-pollinated flowers, since PCR analysis could not allowed distinguishing between the S-c and S-8 alleles. Self-incompatible genotypes were assigned to their corresponding self-incompatibility groups. The knowledge of incompatibility relationships between apricot cultivars can be a highly valuable tool for the development of future breeding programs by selecting the appropriate parents and for efficient orchard design by planting self-compatible and inter-compatible cultivars.
publishDate 2018
dc.date.none.fl_str_mv 2018
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dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/77026
url http://zaguan.unizar.es/record/77026
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/ES/INIA/RTA2014-00085-00
info:eu-repo/grantAgreement/ES/INIA/RTA2017-00003-00
info:eu-repo/grantAgreement/ES/MICINN/AGL2015-74071-JIN
info:eu-repo/grantAgreement/ES/MICINN/AGL2016-77267-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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